|Evaluation of the anti-myocardial ischemia effect of individual and combined extracts of Panax notoginseng and Carthamus tinctorius in rats|
|Han, Shu-Yan1,2; Li, Hai-Xia1; Ma, Xu1; Zhang, Ke1; Ma, Zhi-Zhong3; Jiang, Yong1; Tu, Peng-Fei1|
|关键词||Panax Notoginseng Carthamus Tinctorius Myocardial Infarction Heart Function Oxidative Stress Inflammatory Cytokines|
|刊名||JOURNAL OF ETHNOPHARMACOLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy|
|研究领域[WOS]||Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine|
|关键词[WOS]||OXIDATIVE STRESS ; HEART-FAILURE ; CARDIOVASCULAR-DISEASE ; REPERFUSION INJURY ; INFARCTION ; DYSFUNCTION ; COMPONENTS|
Ethnopharmacological relevance: The decoction of combined Panax notoginseng (Burk) F.H. Chen and Carthamus tinctorius L. has a history of use in traditional medicine for the prevention and treatment of cardiovascular diseases such as angina pectoris and myocardial infarction.
Aim of the study: In this study, we investigated the effects of individual herbal extracts and combined extracts on anti-myocardial ischemia injuries in vivo, and determined the proper dosage of Panax notoginseng (EPN) combined with Carthamus tinctorius (ECT) that could strengthen their cardioprotective effects. Meanwhile, their potential anti-oxidative stress and anti-inflammation effect were assessed.
Material and Methods: SD rats were orally given individual EPN 50, 100 mg/kg, ECT 100,200 mg/kg, and different combinations between them. Myocardial infarction was produced by occlusion of the left anterior descending coronary artery for 24 h. Infarct area was determined with 2,3,5-triphenyltetrazolium chloride (TTC) staining. The biomarkers related to myocardial ischemia injury were determined. Simultaneously, hemodynamic parameters were monitored as left ventricular systolic pressure (LVSP), LV end-diastolic pressure (LVEDP) and maximal rate of increase and decrease of left ventricular pressure (dP/dt(max)). The oxidative stress indicators and inflammatory factors were also evaluated.
Results: The results showed EPN or ECT significantly reduced infarct size, improved cardiac function, decreased levels of creatine kinase (CK) and lactate dehydrogenase (LDH) (all P < 0.05 vs. control). EPN or ECT alone also restrained the oxidative stress related to myocardial ischemia injury as evidenced by decreased malondialdehyde (MDA) and elevated superoxide dismutase (SOD) activity (all P < 0.05 vs. control). However, this cardio-protective effect was further strengthened by their combinations. Among all the combinations, EPN 50 mg/kg plus ECT 200 mg/kg showed predominant potential to reduce infarct size (22.21 +/- 1.72%, P < 0.05 vs. each single, respectively), preserve cardiac function (P < 0.05 vs. ECT 200 mg/kg for LVEDP and -dP/dt(max)) after myocardial ischemia injury in rats. This heart protection was confirmed with the lowered cardiac troponin I (cTnI) (P < 0.05 vs. ECT 200 mg/kg and EPN 50 mg/kg, respectively). Oxidative stress and inflammation are the two key factors in the pathogenesis of myocardial ischemia injury. In the present study, EPN 50 mg/kg plus ECT 200 mg/kg markedly increased SOD and GSH-Px activity (475.30 +/- 23.60 U/ml, P < 0.05 vs. each single, respectively), while elevated MDA level was significantly depressed. Meanwhile, the inflammatory cascade was inhibited as evidenced by decreased cytokines such as tumor necrosis factor-alpha (TNF-alpha), C-reactive protein (CRP) and interleukin-1 beta (IL-1 beta).
Conclusion: These results demonstrated EPN, Ea and their combinations exhibited significant cardioprotective effects. The findings suggest EPN combined with ECT may be therapeutically more useful for ameliorating anti-myocardial ischemia injuries than individual herbal extract, and EPN 50 mg/kg plus ECT 200 mg/kg is the appropriate combination in the present research. The cardio-protective effect of this combination was achieved partially by decreasing oxidative stress and repressing inflammatory cascade. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
|项目编号||30525043 ; 2012ZX09103201-036|
|资助机构||National Science Fund for Distinguished Young Scholars (China) ; National Key Technology R& ; D Program "New Drug Innovation" of China|
|作者单位||1.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China|
2.Peking Univ, Canc Hosp & Inst, Dept Integrat Chinese & Western Med, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Integrat Chinese & Western Med, Beijing 100191, Peoples R China
|Han, Shu-Yan,Li, Hai-Xia,Ma, Xu,et al. Evaluation of the anti-myocardial ischemia effect of individual and combined extracts of Panax notoginseng and Carthamus tinctorius in rats[J]. JOURNAL OF ETHNOPHARMACOLOGY,2013,145(3):722-727.|
|APA||Han, Shu-Yan.,Li, Hai-Xia.,Ma, Xu.,Zhang, Ke.,Ma, Zhi-Zhong.,...&Tu, Peng-Fei.(2013).Evaluation of the anti-myocardial ischemia effect of individual and combined extracts of Panax notoginseng and Carthamus tinctorius in rats.JOURNAL OF ETHNOPHARMACOLOGY,145(3),722-727.|
|MLA||Han, Shu-Yan,et al."Evaluation of the anti-myocardial ischemia effect of individual and combined extracts of Panax notoginseng and Carthamus tinctorius in rats".JOURNAL OF ETHNOPHARMACOLOGY 145.3(2013):722-727.|