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学科主题临床医学
Translocation of classical PKC and cortical granule exocytosis of human oocyte in germinal vesicle and metaphase II stage
Wu, Xue-qing1; Zhang, Xiao1; Li, Xiao-hong1; Cheng, Hui-hong1; Kuai, Yan-rong1; Wang, Song1; Guo, Ying-lu1
关键词Cortical Granule Human Oocyte Protein Kinase c
刊名ACTA PHARMACOLOGICA SINICA
2006-10-01
DOI10.1111/j.1745-7254.2006.00407.x
27期:10页:1353-1358
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]PROTEIN-KINASE-C ; MOUSE EGG ACTIVATION ; CHAETOPTERUS OOCYTES ; MEIOSIS RESUMPTION ; MEIOTIC RESUMPTION ; POLYSPERMY BLOCK ; CELL-CYCLE ; FERTILIZATION ; INVOLVEMENT ; MATURATION
英文摘要

Aim: Protein kinase C (PKC) is as a family of serine/threonine kinases that can be activated by Ca2+, phospholipid and diacylglycerol. PKC plays an important role in oocyte maturation and activation. This study was undertaken to investigate classical PKC (cPKC) in human oocyte maturation and activation. Methods: Germinal vesicle (GV) and metaphase II (MII) stage oocytes were collected from healthy women. The expression and distribution of cPKC were investigated by immunoflourescence. MII oocytes were treated with PKC activator or inhibitor and imaged using a laser confocal scanning microscope (LCSM). Results: In GV oocytes, PKC alpha, beta 1 and gamma were localized to the germinal vesicles, with a weak expression in ooplasm. In MII oocytes, PKC alpha, beta 1 and gamma were distributed evenly in ooplasm. After treatment with PKC activator, phorbol 12-myristate 13-acetate (PMA), cPKC translocated to the periphery of oocyte, and cortical granules (CG) exocytosis was found. When the oocytes were treated with PKC inhibitor, staurosporine, no translocation of cPKC and CG exocytosis were found. Conclusion: PKC alpha, beta 1 and gamma exist in human oocytes and activation of these sub-units could induce CG exocytosis in MII stage.

语种英语
WOS记录号WOS:000240685400010
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被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53226
专题北京大学第一临床医学院_泌尿外科
北京大学第一临床医学院_北京大学泌尿外科研究所
作者单位1.Peking Univ, Inst Urol, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 1, Reprod & Genet Ctr, Beijing 100034, Peoples R China
3.Shanxi Med Univ, Hosp 2, Dept Obstet & Gynecol, Taiyuan 030001, Peoples R China
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GB/T 7714
Wu, Xue-qing,Zhang, Xiao,Li, Xiao-hong,et al. Translocation of classical PKC and cortical granule exocytosis of human oocyte in germinal vesicle and metaphase II stage[J]. ACTA PHARMACOLOGICA SINICA,2006,27(10):1353-1358.
APA Wu, Xue-qing.,Zhang, Xiao.,Li, Xiao-hong.,Cheng, Hui-hong.,Kuai, Yan-rong.,...&Guo, Ying-lu.(2006).Translocation of classical PKC and cortical granule exocytosis of human oocyte in germinal vesicle and metaphase II stage.ACTA PHARMACOLOGICA SINICA,27(10),1353-1358.
MLA Wu, Xue-qing,et al."Translocation of classical PKC and cortical granule exocytosis of human oocyte in germinal vesicle and metaphase II stage".ACTA PHARMACOLOGICA SINICA 27.10(2006):1353-1358.
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