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学科主题: 临床医学
题名:
Translocation of classical PKC and cortical granule exocytosis of human oocyte in germinal vesicle and metaphase II stage
作者: Wu, Xue-qing1; Zhang, Xiao1; Li, Xiao-hong1; Cheng, Hui-hong1; Kuai, Yan-rong1; Wang, Song1; Guo, Ying-lu1
关键词: cortical granule ; human ; oocyte ; protein kinase C
刊名: ACTA PHARMACOLOGICA SINICA
发表日期: 2006-10-01
DOI: 10.1111/j.1745-7254.2006.00407.x
卷: 27, 期:10, 页:1353-1358
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: PROTEIN-KINASE-C ; MOUSE EGG ACTIVATION ; CHAETOPTERUS OOCYTES ; MEIOSIS RESUMPTION ; MEIOTIC RESUMPTION ; POLYSPERMY BLOCK ; CELL-CYCLE ; FERTILIZATION ; INVOLVEMENT ; MATURATION
英文摘要:

Aim: Protein kinase C (PKC) is as a family of serine/threonine kinases that can be activated by Ca2+, phospholipid and diacylglycerol. PKC plays an important role in oocyte maturation and activation. This study was undertaken to investigate classical PKC (cPKC) in human oocyte maturation and activation. Methods: Germinal vesicle (GV) and metaphase II (MII) stage oocytes were collected from healthy women. The expression and distribution of cPKC were investigated by immunoflourescence. MII oocytes were treated with PKC activator or inhibitor and imaged using a laser confocal scanning microscope (LCSM). Results: In GV oocytes, PKC alpha, beta 1 and gamma were localized to the germinal vesicles, with a weak expression in ooplasm. In MII oocytes, PKC alpha, beta 1 and gamma were distributed evenly in ooplasm. After treatment with PKC activator, phorbol 12-myristate 13-acetate (PMA), cPKC translocated to the periphery of oocyte, and cortical granules (CG) exocytosis was found. When the oocytes were treated with PKC inhibitor, staurosporine, no translocation of cPKC and CG exocytosis were found. Conclusion: PKC alpha, beta 1 and gamma exist in human oocytes and activation of these sub-units could induce CG exocytosis in MII stage.

语种: 英语
WOS记录号: WOS:000240685400010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53226
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ, Inst Urol, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 1, Reprod & Genet Ctr, Beijing 100034, Peoples R China
3.Shanxi Med Univ, Hosp 2, Dept Obstet & Gynecol, Taiyuan 030001, Peoples R China

Recommended Citation:
Wu, Xue-qing,Zhang, Xiao,Li, Xiao-hong,et al. Translocation of classical PKC and cortical granule exocytosis of human oocyte in germinal vesicle and metaphase II stage[J]. ACTA PHARMACOLOGICA SINICA,2006,27(10):1353-1358.
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