IR@PKUHSC  > 北京大学第三临床医学院  > 血液内科
学科主题临床医学
A new classification of interphase nuclei based on spatial organizations of chromosome 8 and 21 for t(8;21) (q22;q22) acute myeloid leukemia by three-dimensional fluorescence in situ hybridization
Tian, Xueli1; Wang, Yanfang2; Zhao, Fengying1; Liu, Jinlin3; Yin, Jun1; Chen, Dieyan1; Ma, Wanyun1,4; Ke, Xiaoyan2
关键词3d-fish Acute Myeloid Leukemia Chromosome Pair Translocation Prognosis
刊名LEUKEMIA RESEARCH
2015-12-01
DOI10.1016/j.leukres.2015.09.013
39期:12页:1414-1420
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Hematology
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Oncology ; Hematology
关键词[WOS]ACUTE MYELOGENOUS LEUKEMIA ; MINIMAL RESIDUAL DISEASE ; PROXIMITY ; FUSION ; GENES ; CELLS ; ARCHITECTURE ; CYTOGENETICS ; 3D-FISH ; AML1
英文摘要

Interphase heterogenous chromosomes spatially close to each other are predominantly located near the center of nuclei and are prone to incur translocations. We screened a t(8;21) (q22;q22) acute myeloid leukemia-M2 patient during three phases (post-chemotherapy, remittent stage, and relapse) and a donor of normal karyotype as control by two-(2D) and three-dimensional (3D)-fluorescence in situ hybridization (FISH). Our classification of nuclei (normal, transitional, and malignant nuclei) by 3D-FISH analyses may provide a more precise prognosis than 2D-FISH results, especially for remittent stage sample in our study, in which 2D-FISH findings showed normal results, whereas 3D-FISH results showed extreme abnormalities (normal nuclei 27%, transitional nuclei 36%, malignant nuclei 37%). The relative radial positions (dIR) of chromosomes 8 were similar to d/R of chromosomes 21 for the relapse sample. We classified heterogenous chromosome pairs into close pairs and normal pairs based on their relative distances (d′/(2R)). The centers of close pairs were more internal than normal pairs in nuclei in all samples, and the dIR values of a given-type pairwise heterogenous chromosomes were similar among four samples. Our data demonstrate that the classification of nuclei based on spatial organization of chromosomes by 3D-FISH is reasonable and essential for evaluating acute myeloid leukemia prognosis. (C) 2015 Elsevier Ltd. All rights reserved.

语种英语
所属项目编号11374180
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000365335200016
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53243
专题北京大学第三临床医学院_血液内科
作者单位1.Tsinghua Univ, Dept Automat, Beijing 100084, Peoples R China
2.Collaborat Innovat Ctr Quantum Matter, Beijing, Peoples R China
3.Tsinghua Univ, Dept Phys, State Key Lab Low Dimens Quantum Phys, Beijing 100084, Peoples R China
4.Peking Univ, Hosp 3, Dept Hematol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Tian, Xueli,Wang, Yanfang,Zhao, Fengying,et al. A new classification of interphase nuclei based on spatial organizations of chromosome 8 and 21 for t(8;21) (q22;q22) acute myeloid leukemia by three-dimensional fluorescence in situ hybridization[J]. LEUKEMIA RESEARCH,2015,39(12):1414-1420.
APA Tian, Xueli.,Wang, Yanfang.,Zhao, Fengying.,Liu, Jinlin.,Yin, Jun.,...&Ke, Xiaoyan.(2015).A new classification of interphase nuclei based on spatial organizations of chromosome 8 and 21 for t(8;21) (q22;q22) acute myeloid leukemia by three-dimensional fluorescence in situ hybridization.LEUKEMIA RESEARCH,39(12),1414-1420.
MLA Tian, Xueli,et al."A new classification of interphase nuclei based on spatial organizations of chromosome 8 and 21 for t(8;21) (q22;q22) acute myeloid leukemia by three-dimensional fluorescence in situ hybridization".LEUKEMIA RESEARCH 39.12(2015):1414-1420.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Tian, Xueli]的文章
[Wang, Yanfang]的文章
[Zhao, Fengying]的文章
百度学术
百度学术中相似的文章
[Tian, Xueli]的文章
[Wang, Yanfang]的文章
[Zhao, Fengying]的文章
必应学术
必应学术中相似的文章
[Tian, Xueli]的文章
[Wang, Yanfang]的文章
[Zhao, Fengying]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。