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Identification of biomarkers for melamine-induced nephrolithiasis in young children based on ultra high performance liquid chromatography coupled to time-of-flight mass spectrometry (U-HPLC-Q-TOF/MS)
Duan, Hejun2; Guan, Na1; Wu, Yongning3; Zhang, Jing2; Ding, Jie1; Shao, Bing2
关键词Melamine Exposure Biomarker Nephrolithiasis U-hplc-q-tof/ms
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2011-11-15
DOI10.1016/j.jchromb.2011.09.039
879期:30页:3544-3550
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]METABONOMICS ; FORMULA ; STONES ; URINE ; ACID
英文摘要

Milk products contaminated with melamine caused renal disease in young children in mainland China in 2008. The present study was designed to identify potential markers and assess the underlying metabolomic mechanisms of melamine-induced nephrolithiasis in young children. Urine samples were collected from healthy children (n = 74) and from children diagnosed with nephrolithiasis (n = 73) with either a positive (n = 40) or a negative (n = 33) history of melamine exposure. Ultra high-performance liquid chromatography coupled to time of flight mass spectrometry (U-HPLC-MS/MS) was applied to profile the abundances of metabolites. Partial least squares-discriminant analysis (PLS-DA) was used to discriminate between the samples. Seven compounds were found to highly discriminate between healthy controls and nephrolithiasis patients with a history of melamine exposure. The critical markers such as proline and 5C-aglycone were the predominant markers in the control group and detected only rarely in nephrolithiasis patients with a history of melamine exposure. In contrast, hypoxanthine at was the most significant compound that distinguished nephrolithiasis patients with a history of melamine exposure. It was increased to 116.12 +/- 23.34 mu g/L (mean +/- S.D.) in the melamine-induced nephrolithiasis group, whereas the non-melamine group was at the level of 67.47 +/- 9.33 mu g/L (p < 0.001). The biomarkers for melamine-induced nephrolithiasis identified by this study may have clinical application in determining the aetiology of renal disease in young children. (C) 2011 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000297396200026
项目编号200902009 ; 2011BAK10B06
资助机构Science Research Foundation of Ministry of Health of the People&prime ; s Republic of China ; National Key Technology RD Program ; Beijing Municipal Senior Technical Training Plan in Health System
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53253
专题北京大学第一临床医学院
北京大学第一临床医学院_儿科
作者单位1.Peking Univ, Hosp 1, Beijing 100034, Peoples R China
2.Beijing Ctr Dis Prevent & Control, Beijing Key Lab Diagnost & Traceabil Technol Food, Beijing 100013, Peoples R China
3.China Ctr Dis Control & Prevent, Inst Nutr & Food Safety, Beijing 100021, Peoples R China
推荐引用方式
GB/T 7714
Duan, Hejun,Guan, Na,Wu, Yongning,et al. Identification of biomarkers for melamine-induced nephrolithiasis in young children based on ultra high performance liquid chromatography coupled to time-of-flight mass spectrometry (U-HPLC-Q-TOF/MS)[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(30):3544-3550.
APA Duan, Hejun,Guan, Na,Wu, Yongning,Zhang, Jing,Ding, Jie,&Shao, Bing.(2011).Identification of biomarkers for melamine-induced nephrolithiasis in young children based on ultra high performance liquid chromatography coupled to time-of-flight mass spectrometry (U-HPLC-Q-TOF/MS).JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,879(30),3544-3550.
MLA Duan, Hejun,et al."Identification of biomarkers for melamine-induced nephrolithiasis in young children based on ultra high performance liquid chromatography coupled to time-of-flight mass spectrometry (U-HPLC-Q-TOF/MS)".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879.30(2011):3544-3550.
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