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学科主题: 临床医学
题名:
Identification of biomarkers for melamine-induced nephrolithiasis in young children based on ultra high performance liquid chromatography coupled to time-of-flight mass spectrometry (U-HPLC-Q-TOF/MS)
作者: Duan, Hejun2; Guan, Na1; Wu, Yongning3; Zhang, Jing2; Ding, Jie1; Shao, Bing2
关键词: Melamine exposure ; Biomarker ; Nephrolithiasis ; U-HPLC-Q-TOF/MS
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2011-11-15
DOI: 10.1016/j.jchromb.2011.09.039
卷: 879, 期:30, 页:3544-3550
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: METABONOMICS ; FORMULA ; STONES ; URINE ; ACID
英文摘要:

Milk products contaminated with melamine caused renal disease in young children in mainland China in 2008. The present study was designed to identify potential markers and assess the underlying metabolomic mechanisms of melamine-induced nephrolithiasis in young children. Urine samples were collected from healthy children (n = 74) and from children diagnosed with nephrolithiasis (n = 73) with either a positive (n = 40) or a negative (n = 33) history of melamine exposure. Ultra high-performance liquid chromatography coupled to time of flight mass spectrometry (U-HPLC-MS/MS) was applied to profile the abundances of metabolites. Partial least squares-discriminant analysis (PLS-DA) was used to discriminate between the samples. Seven compounds were found to highly discriminate between healthy controls and nephrolithiasis patients with a history of melamine exposure. The critical markers such as proline and 5C-aglycone were the predominant markers in the control group and detected only rarely in nephrolithiasis patients with a history of melamine exposure. In contrast, hypoxanthine at was the most significant compound that distinguished nephrolithiasis patients with a history of melamine exposure. It was increased to 116.12 +/- 23.34 mu g/L (mean +/- S.D.) in the melamine-induced nephrolithiasis group, whereas the non-melamine group was at the level of 67.47 +/- 9.33 mu g/L (p < 0.001). The biomarkers for melamine-induced nephrolithiasis identified by this study may have clinical application in determining the aetiology of renal disease in young children. (C) 2011 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 200902009 ; 2011BAK10B06
项目资助者: Science Research Foundation of Ministry of Health of the People&prime ; s Republic of China ; National Key Technology RD Program ; Beijing Municipal Senior Technical Training Plan in Health System
WOS记录号: WOS:000297396200026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53253
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Beijing 100034, Peoples R China
2.Beijing Ctr Dis Prevent & Control, Beijing Key Lab Diagnost & Traceabil Technol Food, Beijing 100013, Peoples R China
3.China Ctr Dis Control & Prevent, Inst Nutr & Food Safety, Beijing 100021, Peoples R China

Recommended Citation:
Duan, Hejun,Guan, Na,Wu, Yongning,et al. Identification of biomarkers for melamine-induced nephrolithiasis in young children based on ultra high performance liquid chromatography coupled to time-of-flight mass spectrometry (U-HPLC-Q-TOF/MS)[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(30):3544-3550.
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