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学科主题: 药学
题名:
Simultaneous determination of albiflorin and paeoniflorin in rat urine by solid-phase extraction and high-performance liquid chromatography following oral administration of Si-Wu decoction
作者: Sheng, YX1; Li, L1; Zhang, JL1; Guo, D1
关键词: solid-phase extraction ; albiflorin ; paeoniflorin ; Si-Wu decoction
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 2004-12-01
DOI: 10.1002/bmc.389
卷: 18, 期:10, 页:785-790
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: PAEONIAE RADIX ; GLYCYRRHIZIN ; MICE ; PHARMACOKINETICS ; MUSCLE ; ROOT
英文摘要:

A high performance liquid chromatographic method (HPLC), together with solid phase extraction (SPE), was developed for simultaneous determination of albiflorin and paeoniflorin in rat urine after oral administration of Si-Wu decoction. The samples were pretreated with solid phase extraction using Extract-Clean((TM)) cartridges. Analysis of the extract was performed on A reversed-phase C, column and a mobile phase made up of acetonitrile and 0.03% formic acid (17:83, v/v). UV detection was set at 230 nm. The assay was linear over the range 2.625-52.50 mug/mL for albiflorin and 3.875-77.50 mug/mL for paeoniflorin. The average percentage recoveries of three spiked urines were 97.01 +/- 3.32 and 102.32 +/- 6.97 for albiflorin and paeoniflorin, respectively. The intra-day precision (RSD) ranged from 0.21 to 1.79% at concentrations of 4.20, 10.50, 26.25 and 39.375 mug/mL of albiflorin and 0.12 to 2.92% at concentrations of 3.875, 10.85, 23.25 and 58.125 mug/mL of paeoniflorin, and inter-day precision (RSD) was from 1.02 to 1.86% for albiflorin and 0.94 to 3.30% for paeoniflorin, at the same four concentrations. This method was applied in order to analyze albiflorin and paeoniflorin in rat urine following oral administration of traditional Chinese medicinal preparation of Si-Wu decoction. Copyright (C) 2004 John Wiley Sons, Ltd.

语种: 英语
WOS记录号: WOS:000226044900002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53255
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Peking Univ, Modern Res Ctr Tradit Chinese Med, Beijing 100083, Peoples R China

Recommended Citation:
Sheng, YX,Li, L,Zhang, JL,et al. Simultaneous determination of albiflorin and paeoniflorin in rat urine by solid-phase extraction and high-performance liquid chromatography following oral administration of Si-Wu decoction[J]. BIOMEDICAL CHROMATOGRAPHY,2004,18(10):785-790.
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