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学科主题: 临床医学
题名:
Characterization of LHY-821, a novel moderately differentiated endometrial carcinoma cell line
作者: Hu, Qian; Yu, Li; Chen, Rui; Zhang, Yan; Xie, Ya; Liao, Qinping
关键词: Endometrial adenocarcinoma ; LHY-821 ; Moderately differentiated
刊名: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
发表日期: 2012-08-01
DOI: 10.1007/s11626-012-9518-8
卷: 48, 期:7, 页:441-448
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Developmental Biology
研究领域[WOS]: Cell Biology ; Developmental Biology
关键词[WOS]: BREAST-CANCER ; MUTATIONS ; P53 ; EXPRESSION ; RESISTANCE ; GROWTH
英文摘要:

Endometrial cancer is a major problem for women but only a small number of comprehensively characterized cell models are available for studies. Here, we established a new cell line derived from a Stage IIIc(1) Grade 2 endometrial adenocarcinoma. The cell line, designated LHY-821, was characterized using growth curve, karyotyping, immunohistochemical staining, immunoblotting, drug sensitivity assay, invasion assay, and xenografting in nude mice. LHY-821 has a doubling time of about 46 h and a colony-forming efficiency of approximately 71 %. These cells expresse high levels of progesterone receptor but not estrogen receptor and are sensitive to medroxyprogesterone acetate (MPA). LHY-821 also expresses pan-cytokeratin, PTEN, p53, beta-catenin, IGF-1, and IGF-2. In addition, karyotype analysis revealed that LHY-821 possessed a near diploid karyotype including 6q-, 10p-, Xq-, 13q+, 17p+, and Triplo-12. LHY-821 showed highly tumorigenicity in nude mice (100 %) and weak invasiveness. Chemosensitivity tests showed that LHY-821 was sensitive to both carboplatin and paclitaxel. LHY-821 is an immortalized cell line which had survived more than 80 serial passages; it may provide a novel tool to study the molecular mechanism and potential treatment for endometrial cancer.

语种: 英语
所属项目编号: 30672225
项目资助者: National Natural Science Foundation of P.R China
WOS记录号: WOS:000307509900008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53256
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: Peking Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, Beijing 100034, Peoples R China

Recommended Citation:
Hu, Qian,Yu, Li,Chen, Rui,et al. Characterization of LHY-821, a novel moderately differentiated endometrial carcinoma cell line[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,2012,48(7):441-448.
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