IR@PKUHSC  > 北京大学第一临床医学院
学科主题临床医学
Characterization of LHY-821, a novel moderately differentiated endometrial carcinoma cell line
Hu, Qian; Yu, Li; Chen, Rui; Zhang, Yan; Xie, Ya; Liao, Qinping
关键词Endometrial Adenocarcinoma Lhy-821 Moderately Differentiated
刊名IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
2012-08-01
DOI10.1007/s11626-012-9518-8
48期:7页:441-448
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Developmental Biology
研究领域[WOS]Cell Biology ; Developmental Biology
关键词[WOS]BREAST-CANCER ; MUTATIONS ; P53 ; EXPRESSION ; RESISTANCE ; GROWTH
英文摘要

Endometrial cancer is a major problem for women but only a small number of comprehensively characterized cell models are available for studies. Here, we established a new cell line derived from a Stage IIIc(1) Grade 2 endometrial adenocarcinoma. The cell line, designated LHY-821, was characterized using growth curve, karyotyping, immunohistochemical staining, immunoblotting, drug sensitivity assay, invasion assay, and xenografting in nude mice. LHY-821 has a doubling time of about 46 h and a colony-forming efficiency of approximately 71 %. These cells expresse high levels of progesterone receptor but not estrogen receptor and are sensitive to medroxyprogesterone acetate (MPA). LHY-821 also expresses pan-cytokeratin, PTEN, p53, beta-catenin, IGF-1, and IGF-2. In addition, karyotype analysis revealed that LHY-821 possessed a near diploid karyotype including 6q-, 10p-, Xq-, 13q+, 17p+, and Triplo-12. LHY-821 showed highly tumorigenicity in nude mice (100 %) and weak invasiveness. Chemosensitivity tests showed that LHY-821 was sensitive to both carboplatin and paclitaxel. LHY-821 is an immortalized cell line which had survived more than 80 serial passages; it may provide a novel tool to study the molecular mechanism and potential treatment for endometrial cancer.

语种英语
WOS记录号WOS:000307509900008
项目编号30672225
资助机构National Natural Science Foundation of P.R China
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53256
专题北京大学第一临床医学院
作者单位Peking Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Hu, Qian,Yu, Li,Chen, Rui,et al. Characterization of LHY-821, a novel moderately differentiated endometrial carcinoma cell line[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,2012,48(7):441-448.
APA Hu, Qian,Yu, Li,Chen, Rui,Zhang, Yan,Xie, Ya,&Liao, Qinping.(2012).Characterization of LHY-821, a novel moderately differentiated endometrial carcinoma cell line.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,48(7),441-448.
MLA Hu, Qian,et al."Characterization of LHY-821, a novel moderately differentiated endometrial carcinoma cell line".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 48.7(2012):441-448.
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