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学科主题: 基础医学
题名:
Mechanisms involved in phosphatidylinositol 3-kinase pathway mediated up-regulation of the mu opioid receptor in lymphocytes
作者: Liu, Han1; Li, Hui1; Guo, Liyuan1; Li, Mengsen3; Li, Chaoying1; Wang, Shanshan1; Jiang, Wei1; Liu, Xinhua1; McNutt, Michael A.2; Li, Gang1
关键词: Morphine ; Mu opioid receptor ; PI3K ; Signaling ; Gene expression ; Regulation
刊名: BIOCHEMICAL PHARMACOLOGY
发表日期: 2010-02-01
DOI: 10.1016/j.bcp.2009.09.013
卷: 79, 期:3, 页:516-523
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: SIMIAN IMMUNODEFICIENCY VIRUS ; CEM X174 CELLS ; TRANSCRIPTIONAL REGULATION ; PHOSPHOINOSITIDE 3-KINASE ; PROMOTER REGION ; GENE-EXPRESSION ; IN-VITRO ; APOPTOSIS ; SURVIVAL ; ACTIVATION
英文摘要:

Despite the substantial progress made in understanding initiation expression of the MOR gene in lymphocytes, the signal pathway associated with MOR gene transcription remains to be better defined. As the phosphatidylinositol 3-kinase (PI3K)/AKT pathway can mediate diverse biological responses and is crucial for optimal immune responses and lymphocyte development, this study was undertaken to delineate the role of PI3K/AKT signaling in expression of the MOR gene in CEM x 174 cells. The data show that morphine treatment enhanced the level of phosphorylated, rather than un-phosphorylated, PI3K and AKT, which were synchronously recruited to membrane. The levels of PTEN and p53 which are negative regulators of these signal molecules were reduced, and as a result, the interaction between PTEN and p53 was completely interrupted. With morphine treatment, the levels of both cytoplasmic and nuclear E2F1 which is the downstream effecter of AKT were elevated and the interaction of E2F1 with YY1, rather than Sp1, was also increased. Subsequently, E2F1 triggered the transcription of the MOR gene through its enhanced ability to bind the element in promoter region of the MOR gene. All responses to morphine were abolished by naloxone, which is an antagonist of MOR, or by LY294002, an inhibitor of PI3K, implying specific involvement of PI3K/AKT. These results strongly suggest that the PI3K/AKT pathway plays a critical role in the transfer of signal from morphine stimuli to the machinery by which MOR gene transcription is initiated. (C) 2009 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 30621002 ; 30671856 ; 30772536 ; 20070001735
项目资助者: National Natural Science Foundation of China ; Foundation of National Education Ministry
WOS记录号: WOS:000273694900024
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53257
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: 1.Hainan Med Coll, Key Lab Mol Biol, Haikou 570102, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100083, Peoples R China

Recommended Citation:
Liu, Han,Li, Hui,Guo, Liyuan,et al. Mechanisms involved in phosphatidylinositol 3-kinase pathway mediated up-regulation of the mu opioid receptor in lymphocytes[J]. BIOCHEMICAL PHARMACOLOGY,2010,79(3):516-523.
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