IR@PKUHSC  > 北京大学国际医院  > 胃肠外科
学科主题临床医学
Adipose differentiation-related protein is not involved in hypoxia inducible factor-1-induced lipid accumulation under hypoxia
Shen, Guomin1; Ning, Ning2; Zhao, Xingsheng3; Liu, Xi3; Wang, Guangyu4; Wang, Tianzhen5; Zhao, Ran5; Yang, Chao5; Wang, Dongmei1; Gong, Pingyuan1; Shen, Yan1; Sun, Yongjian1; Zhao, Xiao1; Jin, Yinji5; Yang, Weiwei5; He, Yan5; Zhang, Lei5; Jin, Xiaoming5; Li, Xiaobo5
关键词Adipose Differentiation-related Protein Hypoxia Inducible Factor-1 Lipid Accumulation Hypoxia
刊名MOLECULAR MEDICINE REPORTS
2015-12-01
DOI10.3892/mmr.2015.4488
12期:6页:8055-8061
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Medicine, Research & Experimental
研究领域[WOS]Oncology ; Research & Experimental Medicine
关键词[WOS]DNA-BINDING ACTIVITY ; SMOOTH-MUSCLE-CELLS ; FACTOR-I ; RECEPTOR ; ADIPOPHILIN ; EXPRESSION ; MACROPHAGES ; LDL
英文摘要

Increasing evidence has showed that hypoxia inducible factor-1 (HIF1) has an important role in hypoxia-induced lipid accumulation, a common feature of solid tumors; however, its role remains to be fully elucidated. Adipose differentiation-related protein (ADRP), a structural protein of lipid droplets, is found to be upregulated under hypoxic conditions. In the present study, an MCF7 breast cancer cell line was used to study the role of ADRP in hypoxia-induced lipid accumulation. It was demonstrated that hypoxia induced the gene expression of ADRP in a HIF1-dependent manner. Increases in the mRNA and protein levels of ADRP was accompanied by increased HIF1A activity. In addition, a significant decrease in the mRNA and protein levels of ADRP were detected in presence of siRNA targeting HIF1A. Using a dual-luciferase reporting experiment and chromatin immunoprecipitation assay, the present study demonstrated that ADRP is a direct target gene of HIF1, and identified a functional hypoxia response element localized 33 bp upstream of the transcriptional start site of the ADRP gene. Furthermore, the present study demonstrated the role of ADRP in low density liporotein (LDL) and very-LDL uptake-induced lipid accumulation under hypoxia. The knockdown of ADRP did not reduce HIF1-induced lipid accumulation under hypoxia. Together, these results showed that ADRP may be not involved in HIF1-induced lipid accumulation.

语种英语
WOS记录号WOS:000367189500030
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53273
专题北京大学国际医院_胃肠外科
作者单位1.Henan Univ Sci & Technol, Dept Med Genet, Coll Med, Luoyang 471003, Henan, Peoples R China
2.Peking Univ, Dept Gastrointestinal Surg, Int Hosp, Beijing 100871, Peoples R China
3.Inner Mongolia Peoples Hosp, Dept Cardiovasc Dis, Hohhot 010070, Inner Mongolia, Peoples R China
4.Harbin Med Univ, Dept Gastrointestinal Med Oncol, Affiliated Tumor Hosp, Harbin 100036, Heilongjiang, Peoples R China
5.Harbin Med Univ, Dept Pathol, Harbin 150086, Heilongjiang, Peoples R China
推荐引用方式
GB/T 7714
Shen, Guomin,Ning, Ning,Zhao, Xingsheng,et al. Adipose differentiation-related protein is not involved in hypoxia inducible factor-1-induced lipid accumulation under hypoxia[J]. MOLECULAR MEDICINE REPORTS,2015,12(6):8055-8061.
APA Shen, Guomin.,Ning, Ning.,Zhao, Xingsheng.,Liu, Xi.,Wang, Guangyu.,...&Li, Xiaobo.(2015).Adipose differentiation-related protein is not involved in hypoxia inducible factor-1-induced lipid accumulation under hypoxia.MOLECULAR MEDICINE REPORTS,12(6),8055-8061.
MLA Shen, Guomin,et al."Adipose differentiation-related protein is not involved in hypoxia inducible factor-1-induced lipid accumulation under hypoxia".MOLECULAR MEDICINE REPORTS 12.6(2015):8055-8061.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Shen, Guomin]的文章
[Ning, Ning]的文章
[Zhao, Xingsheng]的文章
百度学术
百度学术中相似的文章
[Shen, Guomin]的文章
[Ning, Ning]的文章
[Zhao, Xingsheng]的文章
必应学术
必应学术中相似的文章
[Shen, Guomin]的文章
[Ning, Ning]的文章
[Zhao, Xingsheng]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。