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Inhibition of TNF-alpha reduces laser-induced choroidal neovascularization
Shi, Xuan1; Semkova, Irina1; Muether, Philipp S.1; Dell, Susanne1; Kociok, Norbert1; Joussen, Antonia M.1
关键词Choroidal Neovascularization Tnf-alpha Leakage
刊名EXPERIMENTAL EYE RESEARCH
2006-12-01
DOI10.1016/j.exer.2006.07.007
83期:6页:1325-1334
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]TUMOR-NECROSIS-FACTOR ; ENDOTHELIAL GROWTH-FACTOR ; RETINAL NEOVASCULARIZATION ; RHEUMATOID-ARTHRITIS ; DIABETIC-RETINOPATHY ; MACULAR DEGENERATION ; ANGIOGENESIS ; CELLS ; EXPRESSION ; RECEPTOR
英文摘要

To investigate the role of the TNF-alpha in the development of laser-induced choroidal neovascularization (CNV) in a mouse model. Four separate laser burns were applied to induce ruptures of Bruch′s membrane and subsequent choroidal neovascularization in C57BL/6J mice. TNF-alpha protein expression was serniquantitatively assessed by Western blot analysis of the choroidal and RPE layer from mice with or without laser treatment. To investigate the effect of TNF-a inhibition on CNV formation, animals were treated for 7 days via intraperitonealy implanted osmotic pumps either 3 days before or after laser injury with recombinant TNF receptor P75 (etanercept), a chimeric monoclonal antibody (infliximab), or a purified rat anti-mouse/rat TNF monoclonal antibody (TNF-mAb), respectively. Fluorescein angiography, flat-mount preparations, and histopathology were performed at day 7, 10, or 14 after laser treatment. Western blotting demonstrated that TNF-alpha expression was 4.57-fold higher in the choroid and RPE one week after laser injury compared to control mice without laser. When evaluated one and two weeks after laser injury, etanercept and infliximab given from the 3rd day before laser-damage significantly reduced CNV size and pathological fluorescein leakage compared to the control group after laser treatment only. The inhibitory effect of the monoclonal TNF-alpha antibody on CNV formation was evident two weeks after photocoagulation but not after one week. Only etanercept administered 3 days after laser injury still reduced significantly the development of CNV lesions. Histopathology confirmed that CNV lesions in treated mice were smaller in size compared to the control animals without TNF inhibitor treatment. In conclusion, anti-TNF-alpha. treatment with different inhibitors reduces both the size and the leakage of laser-induced CNV. These results suggest the involvement of TNF-alpha in the development of laser-induced CNV and its potential use as a therapeutic agent in the age-related macular degeneration. (c) 2006 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000242300700004
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被引频次:80[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53287
专题北京大学第二临床医学院_眼科
作者单位1.Univ Cologne, CMMC, D-5000 Cologne 41, Germany
2.Univ Dusseldorf, Dept Ophthalmol, D-40225 Dusseldorf, Germany
3.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100871, Peoples R China
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GB/T 7714
Shi, Xuan,Semkova, Irina,Muether, Philipp S.,et al. Inhibition of TNF-alpha reduces laser-induced choroidal neovascularization[J]. EXPERIMENTAL EYE RESEARCH,2006,83(6):1325-1334.
APA Shi, Xuan,Semkova, Irina,Muether, Philipp S.,Dell, Susanne,Kociok, Norbert,&Joussen, Antonia M..(2006).Inhibition of TNF-alpha reduces laser-induced choroidal neovascularization.EXPERIMENTAL EYE RESEARCH,83(6),1325-1334.
MLA Shi, Xuan,et al."Inhibition of TNF-alpha reduces laser-induced choroidal neovascularization".EXPERIMENTAL EYE RESEARCH 83.6(2006):1325-1334.
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