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学科主题: 临床医学
题名:
Long-term effect of early discharge on sEPSC and [Ca2+](i) in developing neurons
作者: Wang, JM1; Jiang, YW1; Cao, HY1; Yu, LF1; Bo, T1; Ni, H1; Jiang, Q1; Wu, XR1
关键词: intracellular calcium ; long-term ; seizure ; spontaneous postsynaptic potential
刊名: NEUROSCIENCE LETTERS
发表日期: 2006-04-10
DOI: 10.1016/j.neulet.2005.12.023
卷: 397, 期:1-2, 页:104-109
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: RECURRENT EPILEPTIFORM DISCHARGES ; STROKE-INDUCED EPILEPSY ; IN-VITRO MODEL ; HIPPOCAMPAL-NEURONS ; ACQUIRED EPILEPSY ; RECEPTOR SUBUNITS ; NEONATAL-RATS ; SEIZURES ; MECHANISMS ; EPILEPTOGENESIS
英文摘要:

To study the long-term changes induced in immature rat cortical neuronal cultures by transient exposure to an Mg2+-free treatment, at cultured day 6, cells were assigned into three groups, based on the mediums they were transiently exposed to as follows: control group 1 (CONT1) was exposed to Dulbecco′s Modified Eagle′s Medium (DMEM), control group 2 was exposed to a physiological solution (PS), and the magnesium-free physiological solution group (MGFPS) was exposed to the same medium as CONT2 except for the removal of magnesium. Following a 3-h exposure, the amplitude and frequency of spontaneous excitatory postsynaptic currents (sEPSC) were recorded and intracellular calcium concentrations ([Ca2+](i)) were measured. Compared to the CONT1 and CONT2 groups, the MGFPS group displayed a significantly greater amplitude (at d6, d7, d9, and d12) and frequency (at d6, d7, and d9) of sEPSC (p < 0.05). Also, both the resting and peak intracellular calcium levels were significantly greater in the MGFPS group at days 6, 7, 9, 12 and 17 (p < 0.05). The rise time (time from resting level to peak level of intracellular calcium following NMDA application) was significantly shorter in the MGFPS group at culture days 7 and 17 and significantly longer at culture day 12 (p < 0.05). Finally, we compared the percentage of cortical neurons expressing neuron-specific enolase (NSE) and found that there were no significant differences in the number of NSE positive neurons among three groups at days 7, 12, and 17. Our results suggests that there are long-term changes in sEPSCs and [Ca2+](i) in cultured rat cortical neurons following exposure to Mg2+-free environment without cell loss. (C) 2005 Elsevier Ireland Ltd. All rights

语种: 英语
WOS记录号: WOS:000236241700022
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53291
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: 1.Gen Hosp Chinese PLA, Expt & Analyt Ctr, Beijing, Peoples R China
2.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China

Recommended Citation:
Wang, JM,Jiang, YW,Cao, HY,et al. Long-term effect of early discharge on sEPSC and [Ca2+](i) in developing neurons[J]. NEUROSCIENCE LETTERS,2006,397(1-2):104-109.
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