IR@PKUHSC  > 北京大学基础医学院  > 药理学系
学科主题基础医学
In vitro and in vivo protective effect of Ganoderma lucidum polysaccharides on alloxan-induced pancreatic islets damage
Zhang, HN1; He, JH1; Yuan, L1; Lin, ZB1
关键词Ganoderma Lucidum Polysaccharides Alloxan Pancreatic Islets Free Radicals Nf-kappa b
刊名LIFE SCIENCES
2003-09-19
DOI10.1016/S0024-3205(03)00594-0
73期:18页:2307-2319
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]NF-KAPPA-B ; ACTIVATION ; PREVENTION ; NINHYDRIN ; MICE ; DNA
英文摘要

This study was undertaken to investigate the protective effect against alloxan-induced pancreatic islets damage by Ganoderma lucidum Polysaccharides (Gl-PS) isolated from the fruiting body of Ganoderma lucidum (Leyss. ex Fr.) Karst. In vitro, alloxan caused dose-dependent toxicity on the isolated pancreatic islets. Pre-treatment of islets with Gl-PS for 12 h and 24 h significantly reversed alloxan-induced islets viability loss. Gl-PS was also found to inhibit the free radicals production induced by alloxan in the isolated pancreatic islets using confocal microscopy. Gl-PS dose-dependently increased serum insulin and reduced serum glucose levels when pretreated intragastrically for 10 days in alloxan-induced diabetic mice. It was found that the pancreas homogenates had higher lipid peroxidation products in alloxan-treated mice than in the Gl-PS-treated animals. Aldehyde fuchsin staining revealed that alloxan caused nearly all the beta cells disappearing from the pancreatic islets, while Gl-PS partly protected the beta cells from necrosis. Alloxan (60 mg/kg) induced NF-kappaB activation in the pancreas at 30 min after injection, pretreatment with Gl-PS inhibited alloxan-induced activation of NF-kappaB. These results suggest that Gl-PS was useful in protecting against alloxan-induced pancreatic islets damage in vitro and in vivo; one of the mechanisms is through its scavenging ability to protect the pancreatic islets from free radicals-damage induced by alloxan. (C) 2003 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000185061300004
Citation statistics
Cited Times:66[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53297
Collection北京大学基础医学院_药理学系
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Pharmacol, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Confocal Microscope Lab, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Zhang, HN,He, JH,Yuan, L,et al. In vitro and in vivo protective effect of Ganoderma lucidum polysaccharides on alloxan-induced pancreatic islets damage[J]. LIFE SCIENCES,2003,73(18):2307-2319.
APA Zhang, HN,He, JH,Yuan, L,&Lin, ZB.(2003).In vitro and in vivo protective effect of Ganoderma lucidum polysaccharides on alloxan-induced pancreatic islets damage.LIFE SCIENCES,73(18),2307-2319.
MLA Zhang, HN,et al."In vitro and in vivo protective effect of Ganoderma lucidum polysaccharides on alloxan-induced pancreatic islets damage".LIFE SCIENCES 73.18(2003):2307-2319.
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