北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 肾脏内科  > 期刊论文
学科主题: 临床医学
题名:
Performance evaluation of a novel chemiluminescence assay for detection of anti-GBM antibodies: an international multicenter study
作者: Mahler, Michael1; Radice, Antonella2; Sinico, Renato A.1; Damoiseaux, Jan3; Seaman, Andrea1; Buckmelter, Kristen1; Vizjak, Alenka4; Buchner, Carol1; Binder, Walter L.1; Fritzler, Marvin J.5; Cui, Zhao6
关键词: autoantibodies ; GBM ; Goodpasture&prime ; s syndrome ; rapidly progressive crescentic glomerulonephritis (RPGN)
刊名: NEPHROLOGY DIALYSIS TRANSPLANTATION
发表日期: 2012
DOI: 10.1093/ndt/gfr203
卷: 27, 期:1, 页:243-U295
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Transplantation ; Urology & Nephrology
研究领域[WOS]: Transplantation ; Urology & Nephrology
关键词[WOS]: BASEMENT-MEMBRANE ANTIBODIES ; RAPIDLY PROGRESSIVE GLOMERULONEPHRITIS ; GOODPASTURES-DISEASE ; DIFFERENTIAL-DIAGNOSIS ; CYTOPLASMIC ANTIBODIES ; SEQUENTIAL DEVELOPMENT ; PULMONARY HEMORRHAGE ; AUTOANTIBODIES ; ANCA ; NEPHRITIS
英文摘要:

Background. Autoantibodies to the non-collagen region (NC1) of the alpha-3 subunit of collagen IV represent a serological hallmark in the diagnosis of Goodpasture′s syndrome (GPS). The objective of our study was to carefully analyze the performance characteristics of a novel anti-glomerular basement membrane (GBM) chemiluminescence immunoassay (CIA).

Methods. Sera from patients with GPS (n = 90) were collected from four clinical centers. Samples from different disease groups (n = 397) and healthy individuals (n = 400) were used as controls. All samples were tested for anti-GBM antibodies by a rapid, random access CIA (QUANTA Flash (TM) GBM). Most of the samples were also tested using other methods including different commercial anti-GBM IgG assays and research assays for anti-GBM IgA and IgM.

Results. The sensitivity and specificity of the novel CIA was 95.6% [95% confidence interval (CI) 89.0-98.8%] and 99.6% (95% CI 98.9-99.9%), respectively. Receiver operating characteristic analysis showed good discrimination between GPS patients and controls. The area under the curve was 0.98 (CI 0.96-1.0). The three anti-GBM antibody-positive samples from the control group were from two healthy individuals and one human immunodeficiency virus (HIV)-infected patient. All three individuals had low levels of anti-GBM antibodies [20, 24 and 25 chemiluminescent unit (CU), cutoff 20 CU]. When the results of the new CIA were compared to other methods, good agreement was observed: 95.8% (kappa = 0.92) versus EliA (TM) GBM, 97.4% (kappa = 0.95) versus both BINDAZYME (TM) Anti-GBM and QUANTA Lite (R) GBM. Anti-GBM IgA was detectable in low concentrations in patients with GPS and was associated with anti-GBM IgG but was less useful in discriminating GPS patients and controls. No discrimination was found for anti-GBM IgM.

Conclusion. The novel QUANTA Flash (TM) GBM CIA demonstrated good sensitivity and specificity and had good agreement with other methods. Our data confirm that similar to 5% of patients with GPS do not have detectable levels of anti-GBM antibodies.

语种: 英语
WOS记录号: WOS:000299957700039
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53301
Appears in Collections:北京大学第一临床医学院_肾脏内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.San Carlo Borromeo Hosp, Inst Microbiol, Milan, Italy
2.Univ Ljubljana, Fac Med, Inst Pathol, Ljubljana, Slovenia
3.Univ Calgary, Dept Med, Calgary, AB, Canada
4.INOVA Diagnost INC, San Diego, CA USA
5.Maastricht Univ, Clin Immunol Lab, Med Ctr, Maastricht, Netherlands
6.Peking Univ, Div Renal, Hosp 1, Inst Nephrol, Beijing 100871, Peoples R China

Recommended Citation:
Mahler, Michael,Radice, Antonella,Sinico, Renato A.,et al. Performance evaluation of a novel chemiluminescence assay for detection of anti-GBM antibodies: an international multicenter study[J]. NEPHROLOGY DIALYSIS TRANSPLANTATION,2012,27(1):243-U295.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Mahler, Michael]'s Articles
[Radice, Antonella]'s Articles
[Sinico, Renato A.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Mahler, Michael]‘s Articles
[Radice, Antonella]‘s Articles
[Sinico, Renato A.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace