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学科主题临床医学
Further evidence of endogenous hydrogen sulphide as a mediator of relaxation in human and rat bladder
Gai, Jun-Wei1,2; Wahafu, Wasilijiang3; Guo, Hui4; Liu, Miao5; Wang, Xu-Chang1,2; Xiao, Yun-Xiang1,2; Zhang, Liang6; Xin, Zhong-Cheng2,7; Jin, Jie1,2
关键词Cystathionine Beta-synthase Cystathionine Gamma Lyase Detrusor Hydrogen Sulphide Lower Urinary Tract Symptoms 3-mercaptopyruvate Sulphur Transferase
刊名ASIAN JOURNAL OF ANDROLOGY
2013-09-01
DOI10.1038/aja.2013.32
15期:5页:692-696
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Andrology ; Urology & Nephrology
研究领域[WOS]Endocrinology & Metabolism ; Urology & Nephrology
关键词[WOS]BENIGN PROSTATIC HYPERPLASIA ; SMOOTH-MUSCLE RELAXATION ; OVERACTIVE BLADDER ; DETRUSOR MUSCLE ; URINARY-BLADDER ; CHANNEL ; H2S ; VASORELAXANT ; SYMPTOMS ; LUTS
英文摘要

We investigated the expression of hydrogen sulphide (H2S) in human and rat lower urinary tract (including bladder, prostate and urethra) tissues, and we sought to determine whether H2S induces relaxation of human and Sprague-Dawley (SD) rat bladder strips. Human normal lower urinary tract tissue was obtained for the evaluation of endogenous H2S productivity using a sulphide-sensitive electrode and for the analysis of the expression levels of all three synthases of endogenous H2S, cystathionine beta-synthase (CBS), cystathionine gamma lyase (CSE) and 3-mercaptopyruvate sulphur transferase (MPST, as known as 3-MST) by Western blot assay. CBS, CSE and MPST were located in human sample slides by immunohistochemistry. Human and male adult SD rat bladder strips were tested for H2S function with a transducer and recorded. All experiments were repeated six times. The endogenous H2S productivity and the H2S synthases had various distributions in the human and rat lower urinary tract tissues and were located in both epithelial and stromal sections. L-cysteine (L-Cys, a substrate of CBS, CSE and MPST) elicited relaxation in a dose-dependent manner on human bladder strips pre-contracted by acetylcholine chloride. This effect could be diminished by the ATP-sensitive potassium ion (K-ATP) channel blocker glibenclamide (GLB), the CSE inhibitor DL-propargylglycine (PPG) and the CBS inhibitor hydroxylamine (HA). H2S and its three synthases were present in the human and rat lower urinary tract tissues and relaxed human and rat bladder strips, which implied that endogenous H2S might play a role in physiological function and pathological disorders of the lower urinary tract symptoms (LUTS) or overactive bladder (OAB).

语种英语
WOS记录号WOS:000323933000025
项目编号30571851 ; 81201527
资助机构National Natural Science Foundation of China
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53311
专题北京大学第一临床医学院_泌尿外科
北京大学第三临床医学院_麻醉科
作者单位1.Peking Univ, Dept Urol, Hosp 1, Beijing 100034, Peoples R China
2.Peking Univ, Inst Urol, Beijing 100034, Peoples R China
3.Peoples Liberat Army Gen Hosp, Dept Urol, Beijing 100853, Peoples R China
4.Tsinghua Univ, Hosp 1, Dept Urol, Beijing 100016, Peoples R China
5.Hedong Ctr Dis Control & Prevent, Tianjin 300151, Peoples R China
6.Peking Univ, Clin Coll 4, Beijing Jishuitan Hosp, Dept Orthopaed Surg, Beijing 100035, Peoples R China
7.Peking Univ, Hosp 1, Androl Ctr, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Gai, Jun-Wei,Wahafu, Wasilijiang,Guo, Hui,et al. Further evidence of endogenous hydrogen sulphide as a mediator of relaxation in human and rat bladder[J]. ASIAN JOURNAL OF ANDROLOGY,2013,15(5):692-696.
APA Gai, Jun-Wei.,Wahafu, Wasilijiang.,Guo, Hui.,Liu, Miao.,Wang, Xu-Chang.,...&Jin, Jie.(2013).Further evidence of endogenous hydrogen sulphide as a mediator of relaxation in human and rat bladder.ASIAN JOURNAL OF ANDROLOGY,15(5),692-696.
MLA Gai, Jun-Wei,et al."Further evidence of endogenous hydrogen sulphide as a mediator of relaxation in human and rat bladder".ASIAN JOURNAL OF ANDROLOGY 15.5(2013):692-696.
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