学科主题临床医学
Multicentre evaluation of the Elecsys(A (R)) hepatitis B surface antigen II assay for detection of HBsAg in comparison with other commercially available assays
Jia, Ji-Dong1; Hong, Ma1; Wei, Lai2; Zhang, Xin-Xin3; Mao, Yuan-Li4; Wang, Lan-Lan5; Gao, Zhi-Liang6; Hou, Jin-Lin7; Zhang, Jun8
关键词Hepatitis b Virus Hepatitis b Surface Antigen (Hbsag) Immunoassay Hbv Mutants
刊名MEDICAL MICROBIOLOGY AND IMMUNOLOGY
2009-11-01
DOI10.1007/s00430-009-0127-4
198期:4页:263-269
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology ; Microbiology
研究领域[WOS]Immunology ; Microbiology
关键词[WOS]GENETIC-VARIABILITY ; VIRUS INFECTION ; S-GENE ; SENSITIVITY ; DIAGNOSIS ; IMPACT
英文摘要

Screening for hepatitis B surface antigen (HBsAg) demands highly sensitive and specific immunoassays with the ability to detect clinically relevant surface gene mutants-the presence of which is an increasing concern and can compromise test results. The objective of the study was to compare the clinical and technical performance of the fully automated Elecsys HBsAg II assay with other widely used HBsAg immunoassays in China. This was a multicentre study in which eight Chinese laboratories compared the Elecsys HBsAg II assay with the Architect HBsAg assay, AxSYM HBsAg assay, or generic microtitre plate (MTP) enzyme-linked immunosorbent assays (manufactured in China) against preselected samples, including recombinant surface gene mutants, and routine clinical samples. Elecsys HBsAg II was the most sensitive assay for detecting positive results in seroconversion samples: up to 14, 11, and 22 days earlier than the Architect, AxSYM, and MTP HBsAg assays, respectively. It also detected all 211 preselected HBsAg-positive specimens and all 13 recombinant HBsAg mutants; the AxSYM and MTP HBsAg assays failed to detect 3 and 10 mutant samples, respectively. Sensitivity was 100% for Elecsys HBsAg II with routine samples, compared with 99.1, 98.9, and 95.2% for the Architect, AxSYM, and MTP HBsAg assays, respectively. In conclusion, it was demonstrated that the Elecsys HBsAg II assay is suitable for routine HBsAg screening in China.

语种英语
WOS记录号WOS:000271282500007
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53315
专题北京大学第二临床医学院_北京大学肝病研究所
作者单位1.Beijing 302 Mil Hosp China, Beijing, Peoples R China
2.Fudan Univ, Publ Hlth Clin Ctr, Shanghai 200433, Peoples R China
3.Capital Med Univ, Beijing Friendship Hosp, Beijing 100050, Peoples R China
4.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100871, Peoples R China
5.Shanghai Jiao Tong Univ, Ruijin Hosp, Sch Med, Shanghai 200030, Peoples R China
6.Sichuan Univ, W China Hosp Lab, W China Med Sch, Chengdu 610064, Peoples R China
7.Sun Yat Sen Univ, Affiliated Hosp 3, Guangzhou 510275, Guangdong, Peoples R China
8.So Med Univ, Nanfang Hosp, Guangzhou, Guangdong, Peoples R China
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Jia, Ji-Dong,Hong, Ma,Wei, Lai,et al. Multicentre evaluation of the Elecsys(A (R)) hepatitis B surface antigen II assay for detection of HBsAg in comparison with other commercially available assays[J]. MEDICAL MICROBIOLOGY AND IMMUNOLOGY,2009,198(4):263-269.
APA Jia, Ji-Dong.,Hong, Ma.,Wei, Lai.,Zhang, Xin-Xin.,Mao, Yuan-Li.,...&Zhang, Jun.(2009).Multicentre evaluation of the Elecsys(A (R)) hepatitis B surface antigen II assay for detection of HBsAg in comparison with other commercially available assays.MEDICAL MICROBIOLOGY AND IMMUNOLOGY,198(4),263-269.
MLA Jia, Ji-Dong,et al."Multicentre evaluation of the Elecsys(A (R)) hepatitis B surface antigen II assay for detection of HBsAg in comparison with other commercially available assays".MEDICAL MICROBIOLOGY AND IMMUNOLOGY 198.4(2009):263-269.
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