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学科主题临床医学
Defining Stem and Progenitor Cells within Adipose Tissue
Lin, Guiting1; Garcia, Maurice1; Ning, Hongxiu1; Banie, Lia1; Guo, Ying-Lu2; Lue, Tom F.1; Lin, Ching-Shwun1
刊名STEM CELLS AND DEVELOPMENT
2008-12-01
DOI10.1089/scd.2008.0117
17期:6页:1053-1063
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering ; Hematology ; Medicine, Research & Experimental ; Transplantation
资助者California Urology Foundation ; Mr. Arthur Rock and the Rock Foundation ; National Institutes of Health ; California Urology Foundation ; Mr. Arthur Rock and the Rock Foundation ; National Institutes of Health
研究领域[WOS]Cell Biology ; Hematology ; Research & Experimental Medicine ; Transplantation
关键词[WOS]LYMPHATIC ENDOTHELIAL-CELLS ; STROMAL CELLS ; BONE-MARROW ; IN-VITRO ; POSTNATAL NEOVASCULARIZATION ; ENHANCES DIFFERENTIATION ; REGENERATIVE MEDICINE ; EXPRESSION ; CD34 ; TELOMERASE
英文摘要

Adipose tissue-derived stem cells (ADSC) are routinely isolated from the stromal vascular fraction (SVF) of homogenized adipose tissue. Freshly isolated ADSC display surface markers that differ from those of cultured ADSC, but both cell preparations are capable of multipotential differentiation. Recent studies have inferred that these progenitors may reside in a perivascular location where they appeared to coexpress CD34 and smooth muscle actin (alpha-SMA) but not CD31. However, these studies provided only limited histological evidence to support such assertions. In the present study, we employed immunohistochemistry and immunofluorescence to define more precisely the location of ADSC within human adipose tissue. Our results show that alpha-SMA and CD31 localized within smooth muscle and endothelial cells, respectively, in all blood vessels examined. CD34 localized to both the intima (endothelium) and adventitia neither of which expressed alpha-SMA. The niche marker Wnt5a was confined exclusively to the vascular wall within mural smooth muscle cells. Surprisingly, the widely accepted mesenchymal stem cell marker STRO-1 was expressed exclusively in the endothelium of capillaries and arterioles but not in the endothelium of arteries. The embryonic stem cell marker SSEA1 localized to a pericytic location in capillaries and in certain smooth muscle cells of arterioles. Cells expressing the embryonic stem cell markers telomerase and OCT4 were rare and observed only in capillaries. Based on these findings and evidence gathered from the existing literature, we propose that ADSC are vascular precursor (stem) cells at various stages of differentiation. In their native tissue, ADSC at early stages of differentiation can differentiate into tissue-specific cells such as adipocytes. Isolated, ADSC can be induced to differentiate into additional cell types such as osteoblasts and chondrocytes.

语种英语
资助者California Urology Foundation ; Mr. Arthur Rock and the Rock Foundation ; National Institutes of Health ; California Urology Foundation ; Mr. Arthur Rock and the Rock Foundation ; National Institutes of Health
WOS记录号WOS:000261490700006
Citation statistics
Cited Times:226[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53321
Collection北京大学第一临床医学院_男科中心
作者单位1.Peking Univ, Hosp 1, Androl Ctr, Beijing 100871, Peoples R China
2.Univ Calif San Francisco, Dept Urol, Sch Med, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA
Recommended Citation
GB/T 7714
Lin, Guiting,Garcia, Maurice,Ning, Hongxiu,et al. Defining Stem and Progenitor Cells within Adipose Tissue[J]. STEM CELLS AND DEVELOPMENT,2008,17(6):1053-1063.
APA Lin, Guiting.,Garcia, Maurice.,Ning, Hongxiu.,Banie, Lia.,Guo, Ying-Lu.,...&Lin, Ching-Shwun.(2008).Defining Stem and Progenitor Cells within Adipose Tissue.STEM CELLS AND DEVELOPMENT,17(6),1053-1063.
MLA Lin, Guiting,et al."Defining Stem and Progenitor Cells within Adipose Tissue".STEM CELLS AND DEVELOPMENT 17.6(2008):1053-1063.
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