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Effects of oxymatrine on the serum levels of T helper cell 1 and 2 cytokines and the expression of the S gene in hepatitis B virus S gene transgenic mice: A study on the anti-hepatitis B virus mechanism of oxymatrine
Dong, YH1; Xi, HL1; Yu, YY1; Wang, QH1; Jiang, K1; Li, LZ1
关键词Antiviral Drug Hepatitis b Virus s Gene Immune Response Oxymatrine t Helper Cell 1 And 2 Cytokine Transgenic Mouse
刊名JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
2002-12-01
17期:12页:1299-1306
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]INTERFERON-ALPHA ; RIBAVIRIN ; RESPONSES ; PROLIFERATION ; SECRETION ; PROFILE
英文摘要

Background: Oxymatrine has been shown to have a remarkable inhibitory activity to hepatitis B virus (HBV) infection with a hepatitis B virus e antigen (HBeAg) serum conversion rate of approximately 45%. In order to explore the anti-HBV mechanism of oxymatrine, the effects of oxymatrine on serum levels of T helper (h) 1 cytokines (interferon (IFN)-gamma and interleukin (IL)-2) and Th2 cytokines (IL-4 and IL-10), and the expression of S gene in HBV S gene transgenic mice were studied.

Methods: Each transgenic mouse was either injected with oxymatrine or saline intraperitoneally once a day for 30 days. Serum levels of IFN-gamma, IL-2, IL-4 and IL-10 were quantitated and compared to the data before the treatment. The expression of HBV S gene in transgenic mice was analyzed at the DNA, mRNA and protein levels.

Results: The serum levels of IFN-gamma in transgenic mice before or after oxymatrine treatment were 3.108 +/- 3.172 and 11.059 +/- 6.971 pg/mL, respectively. In contrast, serum levels before and after oxymatrine treatment for IL-4 were 29.045 +/- 13.235 and 13.024 +/- 9.002 pg/mL, respectively (P < 0.001). The serum levels of IL-2 in the control (saline injection) and oxymatrine-treated mice were 1.070 +/- 0.447 and 5.537 +/- 2.887 pg/mL, respectively (P < 0.0001); and that of IL-10 were 97.226 +/- 73.306 and 33.607 +/- 23.154 pg/mL, respectively (P < 0.01). No significant differences were observed in the expression of HBV S gene in the transgenic mice at the DNA, mRNA and protein levels before or after oxymatrine treatment.

Conclusions: The fact that Th1 cytokines are increased while Th2 cytokines are decreased suggests that oxymatrine treatment triggers the change of immune response to hepatitis B infection in transgenic mice, which leads to improved HBV inhibitory activities. The study can help us better understand the mechanisms of the anti-HBV drug, oxymatrine, and how it has potential as an application in clinical chronic hepatitis B treatment. (C) 2002 Blackwell Publishing Asia Pty Ltd.

语种英语
WOS记录号WOS:000179111400010
引用统计
被引频次:33[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53325
专题北京大学第一临床医学院_感染疾病科
作者单位1.Peking Univ, Hosp 1, Dept Infect Dis, Beijing 100034, Peoples R China
2.Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Immunol, Wuhan 430074, Peoples R China
3.Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Parasitol, Wuhan 430074, Peoples R China
推荐引用方式
GB/T 7714
Dong, YH,Xi, HL,Yu, YY,et al. Effects of oxymatrine on the serum levels of T helper cell 1 and 2 cytokines and the expression of the S gene in hepatitis B virus S gene transgenic mice: A study on the anti-hepatitis B virus mechanism of oxymatrine[J]. JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY,2002,17(12):1299-1306.
APA Dong, YH,Xi, HL,Yu, YY,Wang, QH,Jiang, K,&Li, LZ.(2002).Effects of oxymatrine on the serum levels of T helper cell 1 and 2 cytokines and the expression of the S gene in hepatitis B virus S gene transgenic mice: A study on the anti-hepatitis B virus mechanism of oxymatrine.JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY,17(12),1299-1306.
MLA Dong, YH,et al."Effects of oxymatrine on the serum levels of T helper cell 1 and 2 cytokines and the expression of the S gene in hepatitis B virus S gene transgenic mice: A study on the anti-hepatitis B virus mechanism of oxymatrine".JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY 17.12(2002):1299-1306.
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