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学科主题: 口腔医学
题名:
A pilot trial on the molecular pathophysiology of traumatic temporomandibular joint bony ankylosis in a sheep model. Part II: The differential gene expression among fibrous ankylosis, bony ankylosis and condylar fracture
作者: Yan, Ying-Bin1,4; Li, Jiang-Ming1; Xiao, E.1; An, Jin-Gang1; Gan, Ye-Hua2,3; Zhang, Yi1
关键词: Temporomandibular joint ; Ankylosis ; Trauma ; Animal model ; Gene expression ; Sheep
刊名: JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY
发表日期: 2014-03-01
DOI: 10.1016/j.jcms.2013.04.008
卷: 42, 期:2, 页:E23-E28
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine ; Surgery
研究领域[WOS]: Dentistry, Oral Surgery & Medicine ; Surgery
关键词[WOS]: REDUCES OSTEOCYTE EXPRESSION ; MECHANICAL STIMULATION ; 2003 UPDATE ; NONUNION
英文摘要:

Objective: The purpose of the study was to preliminarily explore the differential expressions of a series of genes regulating bone formation in temporomandibular joint (TMJ) fibrous ankylosis, bony ankylosis and condylar fracture healing.

Methods: The cDNA from either the bony ankylosed callus or fracture callus of the 6 sheep, as described in the part I, were both used in the study. The differences of gene expressions between bony ankylosis and condylar fracture at 1, 3, and 6 months postoperatively were measured by real-time PCR, with 2 samples at each time point. In addition, another 2 sheep were added to have fibrous ankylosis induced on the right TMJ, and 1 sheep was sacrificed at 3 and 6 months after surgery, respectively. The differences of gene expressions between fibrous and bony ankylosis at 3 and 6 months postoperatively were measured by real-time PCR.

Results: Bony ankylosis showed higher mRNA expression trends in Wnt2b, Wnt5a, beta-Catenin, Lef1, CyclinD1, Runx2, Osterix, Sox9, Col10a1, Alp, Ocn, Bmp2, and Bmp7 compared to fibrous ankylosis, although no statistical analysis was performed due to the very small sample size. Whereas bony ankylosis showed a significant lower expression of Wnt5a, beta-Catenin, Lef1, Runx2, Osterix, Sox9, Col10a1, Alp, Ocn and Bmp4 compared to condylar fracture at several time points (P < 0.05).

Conclusion: Our data provided a preliminary molecular evidence for the hypothesis that the development of traumatic TMJ bony ankylosis was the course of delayed bone healing or hypertrophic nonunion, and deserved to be further studied. (C) 2013 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 81070808 ; 81170936
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000346971700002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53337
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
2.Peking Univ Sch & Hosp Stomatol, Mol Biol Lab, Beijing 100081, Peoples R China
3.Peking Univ Sch & Hosp Stomatol, Ctr Temporomandibular Disorders & Orofacial Pain, Beijing 100081, Peoples R China
4.Tianjin Stomatol Hosp, Dept Oral & Maxillofacial Surg, Tianjin 300041, Peoples R China

Recommended Citation:
Yan, Ying-Bin,Li, Jiang-Ming,Xiao, E.,et al. A pilot trial on the molecular pathophysiology of traumatic temporomandibular joint bony ankylosis in a sheep model. Part II: The differential gene expression among fibrous ankylosis, bony ankylosis and condylar fracture[J]. JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY,2014,42(2):E23-E28.
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