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学科主题: 临床医学
题名:
Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS
作者: Nie, Honggang1,2,3; Liu, Ranran1,2,3; Yang, Youyou1,2,3; Bai, Yu1,2,3; Guan, Yafeng4; Qian, Daqing5; Wang, Tao6; Liu, Huwei1,2,3
关键词: lipidomic ; free fatty acid ; phospholipid ; lyso-phospholipid ; plasmalogen ; peritoneal dialysis ; peritoneum ; biomarker ; two-dimensional liquid chromatography ; mass spectrometry
刊名: JOURNAL OF LIPID RESEARCH
发表日期: 2010-09-01
DOI: 10.1194/jlr.D007567
卷: 51, 期:9, 页:2833-2844
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ; IONIZATION MASS-SPECTROMETRY ; LIGHT-SCATTERING DETECTION ; CAPILLARY GAS-CHROMATOGRAPHY ; FREE FATTY-ACIDS ; ELECTROSPRAY-IONIZATION ; FRAGMENTATION PROCESSES ; RAPID SEPARATION ; NEUTRAL LIPIDS ; BIOLOGICAL SAMPLES
英文摘要:

An online, two-dimensional (2D) liquid chromatography (LC) quadrupole time-of-flight mass spectrometry (QToF-MS) method was developed for lipid profiling of rat peritoneal surface layers, in which the lipid classes and species could be simultaneously separated in one injection with a significantly increased sensitivity. Different lipid classes were separated on a normal-phase column in the first dimension and lipid molecular species were separated on a reversed-phase column in the second dimension, so that the ion suppression effects were reduced while the detection sensitivity was improved. Identified were 721 endogenous lipid species from 12 lipid classes, in which 415 structures were confirmed using tandem mass spectra, and the other 306 lipid molecular species were identified by accurate masses. The linearity, limit of detection, and repeatability were all satisfactory. The method was applied to the investigation of the lipid changes in rat peritoneal surface layer after peritoneal dialysis, and 32 potential lipid biomarkers were identified, as their concentrations in the dosed group were 2.2-12.5 times of those in the control group. The results revealed that this 2D LC-MS system was a promising tool for lipid profiling of complex biological samples.-Nie, H., R. Liu, Y. Yang, Y. Bai, Y. Guan, D. Qian, T. Wang, and H. Liu. Lipid profiling of rat peritoneal surface layers by online normal-and reversed-phase 2D LC QToF-MS. J. Lipid Res. 2010. 51: 2833-2844.

语种: 英语
所属项目编号: 20975005 ; 90717002 ; 20805001 ; 20775090
项目资助者: National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities
WOS记录号: WOS:000280800200035
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53348
Appears in Collections:北京大学第三临床医学院_肾内科_期刊论文

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作者单位: 1.Peking Univ, Inst Analyt Chem, Beijing 100871, Peoples R China
2.Wannan Med Coll, Dept Med, Wuhu, Anhui, Peoples R China
3.Peking Univ, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
4.Peking Univ, Minist Educ, Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China
5.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumental & Analyt Chem, Beijing 100864, Peoples R China
6.Peking Univ, Hosp 3, Div Nephrol, Beijing 100871, Peoples R China

Recommended Citation:
Nie, Honggang,Liu, Ranran,Yang, Youyou,et al. Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS[J]. JOURNAL OF LIPID RESEARCH,2010,51(9):2833-2844.
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