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学科主题临床医学
Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS
Nie, Honggang1,2,3; Liu, Ranran1,2,3; Yang, Youyou1,2,3; Bai, Yu1,2,3; Guan, Yafeng4; Qian, Daqing5; Wang, Tao6; Liu, Huwei1,2,3
关键词Lipidomic Free Fatty Acid Phospholipid Lyso-phospholipid Plasmalogen Peritoneal Dialysis Peritoneum Biomarker Two-dimensional Liquid Chromatography Mass Spectrometry
刊名JOURNAL OF LIPID RESEARCH
2010-09-01
DOI10.1194/jlr.D007567
51期:9页:2833-2844
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; IONIZATION MASS-SPECTROMETRY ; LIGHT-SCATTERING DETECTION ; CAPILLARY GAS-CHROMATOGRAPHY ; FREE FATTY-ACIDS ; ELECTROSPRAY-IONIZATION ; FRAGMENTATION PROCESSES ; RAPID SEPARATION ; NEUTRAL LIPIDS ; BIOLOGICAL SAMPLES
英文摘要

An online, two-dimensional (2D) liquid chromatography (LC) quadrupole time-of-flight mass spectrometry (QToF-MS) method was developed for lipid profiling of rat peritoneal surface layers, in which the lipid classes and species could be simultaneously separated in one injection with a significantly increased sensitivity. Different lipid classes were separated on a normal-phase column in the first dimension and lipid molecular species were separated on a reversed-phase column in the second dimension, so that the ion suppression effects were reduced while the detection sensitivity was improved. Identified were 721 endogenous lipid species from 12 lipid classes, in which 415 structures were confirmed using tandem mass spectra, and the other 306 lipid molecular species were identified by accurate masses. The linearity, limit of detection, and repeatability were all satisfactory. The method was applied to the investigation of the lipid changes in rat peritoneal surface layer after peritoneal dialysis, and 32 potential lipid biomarkers were identified, as their concentrations in the dosed group were 2.2-12.5 times of those in the control group. The results revealed that this 2D LC-MS system was a promising tool for lipid profiling of complex biological samples.-Nie, H., R. Liu, Y. Yang, Y. Bai, Y. Guan, D. Qian, T. Wang, and H. Liu. Lipid profiling of rat peritoneal surface layers by online normal-and reversed-phase 2D LC QToF-MS. J. Lipid Res. 2010. 51: 2833-2844.

语种英语
WOS记录号WOS:000280800200035
项目编号20975005 ; 90717002 ; 20805001 ; 20775090
资助机构National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities
引用统计
被引频次:53[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53348
专题北京大学第三临床医学院_肾内科
北京大学公共卫生学院_公共卫生学院
作者单位1.Peking Univ, Inst Analyt Chem, Beijing 100871, Peoples R China
2.Wannan Med Coll, Dept Med, Wuhu, Anhui, Peoples R China
3.Peking Univ, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
4.Peking Univ, Minist Educ, Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China
5.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumental & Analyt Chem, Beijing 100864, Peoples R China
6.Peking Univ, Hosp 3, Div Nephrol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Nie, Honggang,Liu, Ranran,Yang, Youyou,et al. Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS[J]. JOURNAL OF LIPID RESEARCH,2010,51(9):2833-2844.
APA Nie, Honggang.,Liu, Ranran.,Yang, Youyou.,Bai, Yu.,Guan, Yafeng.,...&Liu, Huwei.(2010).Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS.JOURNAL OF LIPID RESEARCH,51(9),2833-2844.
MLA Nie, Honggang,et al."Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS".JOURNAL OF LIPID RESEARCH 51.9(2010):2833-2844.
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