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学科主题: 临床医学
题名:
Spinal release of the amino acids with a time course in a rat model of postoperative pain
作者: Wang Yun1; Yue Yun1; Shi Lin1; Wu An-shi1; Feng Chun-sheng1; Ni Cheng1
关键词: postoperative pain ; amino acids ; spinal cord
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2007-11-20
卷: 120, 期:22, 页:1969-1974
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: FREELY MOVING RATS ; RECEPTOR ANTAGONISTS ; INCISIONAL PAIN ; MECHANISMS ; MICRODIALYSIS ; ANALGESIA ; NMDA ; CORD ; CYCLOOXYGENASE-2 ; SENSITIZATION
英文摘要:

Background The mechanisms underlying postoperative pain remain unclear. Neurotransmitters of excitatory and inhibitory amino acids play an important role in the transmission and modulation of pain in the spinal dorsal horn. This study aimed to investigate the changes of release of excitatory and inhibitory amino acids in the spinal cord during postoperative pain and to provide a novel theoretical basis for postoperative pain management.

Methods Loop microdialysis catheters were implanted subarachnoidally via the atlanto-occipital membrane in 16 healthy Sprague-Dawley rats. All rats without neural deficits were divided into two groups, Group A and Group B, following 5 days of recovery. The tubes for microdialysis were connected and 25 mu l microdialysate sample for baseline value was collected after one-hour washout in each rat. A plantar incision in the right hind paws of rats in Group A were performed under 1.2% isoflurane. All rats in Group B were only anesthetized by 1.2% isoflurane for the same duration. The microdialysate samples were collected at 3 hours, 1 day, 2 days and 3 days after the incision (or isoflurane anesthesia in Group B) in both groups. The cumulative pain scores were also assessed at the above time-points. The amino acids in the microdialysate samples were tested using high performance liquid chromatography.

Results Within Group A, the release of aspartate and glutamate at 3 hours after the incision was significantly higher than the baseline values and the release of glycine at 1 day after the incision significantly increased compared with the baseline values (P<0.01). Within Group 13, the release of neurotransmitters at each time point had no significant difference compared with the baseline values (P>0.05). The release of aspartate and glutamate at 3 hours after the incision in Group A was significantly higher than that in Group B (P<0.01). The release of glycine at 1 day after the incision in Group A significantly increased compared with Group B (P<0.01). The cumulative pain scores at 3 hours, 1 day and 2 days after the incision in Group A were significantly higher than those in Group B (P<0.01).

Conclusions The release of the excitatory amino acids occurs in the early phase of postoperative pain and might not be involved in the maintenance of pain in a rat model of incision pain. The release of inhibitory glycine lagged behind the excitatory amino acids. The implication of inhibitory glycine release remained to be established further.

语种: 英语
WOS记录号: WOS:000251298400006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53358
Appears in Collections:北京大学第三临床医学院_麻醉科_期刊论文

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作者单位: 1.Capital Med Univ, Beijing Chaoyang Hosp, Dept Anesthesiol, Beijing 100020, Peoples R China
2.Flemish Free Univ Med Ctr, Dept Anesthesiol, B-1090 Brussels, Belgium
3.Jilin Univ, Hosp 1, Dept Anesthesiol, Changchun 130000, Peoples R China
4.Peking Univ, Hosp 3, Dept Anesthesiol, Beijing 100067, Peoples R China

Recommended Citation:
Wang Yun,Yue Yun,Shi Lin,et al. Spinal release of the amino acids with a time course in a rat model of postoperative pain[J]. CHINESE MEDICAL JOURNAL,2007,120(22):1969-1974.
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