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学科主题: 基础医学
题名:
Increased heme oxygenase-1 expression in infarcted rat hearts following human bone marrow mesenchymal cell transplantation
作者: Zhang, SH1; Lu, S1; Ge, JB1; Guo, JX1; Chen, P1; Li, T1; Zhang, P1; Jia, ZQ1; Ma, KT1; Liu, YG1; Zhou, CY1; Li, LS1
关键词: heme oxygenase-1 (HO-1) ; cytoprotection ; human bone marrow mesenchymal cells ; rat myocardial infiarction ; transplantation
刊名: MICROVASCULAR RESEARCH
发表日期: 2005
DOI: 10.1016/j.mvr.2005.01.004
卷: 69, 期:1-2, 页:64-70
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Peripheral Vascular Disease
研究领域[WOS]: Cardiovascular System & Cardiology
关键词[WOS]: HEAT-SHOCK-PROTEIN ; MYOCARDIAL-INFARCTION ; TRANSGENIC MICE ; STEM-CELLS ; IN-VIVO ; PROTECTION ; INDUCTION ; CARDIOMYOPLASTY ; CARDIOMYOCYTES ; DIFFERENTIATE
英文摘要:

Heme oxygenase-1 (HO-1) plays an important role in oxidative stress and recent studies indicate that it is a graft survival protein in cardiac and liver transplant models. In this study, we investigated the relation between the expressions of HO-1 and the effects of human bone marrow mesenchymal cells (MSCs) transplantation to xenogenic rat hearts with experimental myocardial infarction (MI). A total of 5 x 106 cells in 100 mu l PBS or equal volume PBS alone were injected into the ischemic zones immediately post-MI. At 1, 3, and 7 days post-MI, cardiac function was evaluated by echocardiography, the expression of HO-1 was assessed by real-time PCR and Western blot, the localization of HO-1 protein was determined under immunofluorescence microscopy. The infarct size was examined by histology. The numbers of Hoechst-33342 positive MSCs were evaluated under immunofluorescence microscopy and also by flow cytometry after isolation from the host hearts. The results indicated that the HO-1 expressions were markedly increased at both mRNA and protein levels in comparison with injection of PBS at each time point post MSCs transplantation (P < 0.01). HO-1 was revealed both in transplanted MSCs and recipient cardiomyocytes by immunofluorescence. Up-regulated HO-1 expression was accompanied by increase of the numbers of Hoechst-33342 positive MSCs, the reduction of infarct size, and the improvement of cardiac function. Transplantation of human MSCs could up-regulate HO-1 expression in infarct rat hearts, which might play an important role in protecting transplanted MSCs, cardiomyocytes survival, and cardiac function improvement during the early stage after MI. (c) 2005 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000228229500009
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53362
Appears in Collections:基础医学院_北京大学干细胞研究中心_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Stem Cell Res Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 3, Dept Cardiol, Beijing 100871, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
4.Fudan Univ, Zhongshan Univ, Shanghai Inst Cardiovasc Dis, Shanghai 200433, Peoples R China

Recommended Citation:
Zhang, SH,Lu, S,Ge, JB,et al. Increased heme oxygenase-1 expression in infarcted rat hearts following human bone marrow mesenchymal cell transplantation[J]. MICROVASCULAR RESEARCH,2005,69(1-2):64-70.
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