IR@PKUHSC  > 北京大学基础医学院  > 北京大学干细胞研究中心
学科主题基础医学
Increased heme oxygenase-1 expression in infarcted rat hearts following human bone marrow mesenchymal cell transplantation
Zhang, SH1; Lu, S1; Ge, JB1; Guo, JX1; Chen, P1; Li, T1; Zhang, P1; Jia, ZQ1; Ma, KT1; Liu, YG1; Zhou, CY1; Li, LS1
关键词heme oxygenase-1 (HO-1) cytoprotection human bone marrow mesenchymal cells rat myocardial infiarction transplantation
刊名MICROVASCULAR RESEARCH
2005
DOI10.1016/j.mvr.2005.01.004
69期:1-2页:64-70
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Peripheral Vascular Disease
研究领域[WOS]Cardiovascular System & Cardiology
关键词[WOS]HEAT-SHOCK-PROTEIN ; MYOCARDIAL-INFARCTION ; TRANSGENIC MICE ; STEM-CELLS ; IN-VIVO ; PROTECTION ; INDUCTION ; CARDIOMYOPLASTY ; CARDIOMYOCYTES ; DIFFERENTIATE
英文摘要

Heme oxygenase-1 (HO-1) plays an important role in oxidative stress and recent studies indicate that it is a graft survival protein in cardiac and liver transplant models. In this study, we investigated the relation between the expressions of HO-1 and the effects of human bone marrow mesenchymal cells (MSCs) transplantation to xenogenic rat hearts with experimental myocardial infarction (MI). A total of 5 x 106 cells in 100 mu l PBS or equal volume PBS alone were injected into the ischemic zones immediately post-MI. At 1, 3, and 7 days post-MI, cardiac function was evaluated by echocardiography, the expression of HO-1 was assessed by real-time PCR and Western blot, the localization of HO-1 protein was determined under immunofluorescence microscopy. The infarct size was examined by histology. The numbers of Hoechst-33342 positive MSCs were evaluated under immunofluorescence microscopy and also by flow cytometry after isolation from the host hearts. The results indicated that the HO-1 expressions were markedly increased at both mRNA and protein levels in comparison with injection of PBS at each time point post MSCs transplantation (P < 0.01). HO-1 was revealed both in transplanted MSCs and recipient cardiomyocytes by immunofluorescence. Up-regulated HO-1 expression was accompanied by increase of the numbers of Hoechst-33342 positive MSCs, the reduction of infarct size, and the improvement of cardiac function. Transplantation of human MSCs could up-regulate HO-1 expression in infarct rat hearts, which might play an important role in protecting transplanted MSCs, cardiomyocytes survival, and cardiac function improvement during the early stage after MI. (c) 2005 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000228229500009
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53362
Collection北京大学基础医学院_北京大学干细胞研究中心
作者单位1.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Stem Cell Res Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 3, Dept Cardiol, Beijing 100871, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
4.Fudan Univ, Zhongshan Univ, Shanghai Inst Cardiovasc Dis, Shanghai 200433, Peoples R China
Recommended Citation
GB/T 7714
Zhang, SH,Lu, S,Ge, JB,et al. Increased heme oxygenase-1 expression in infarcted rat hearts following human bone marrow mesenchymal cell transplantation[J]. MICROVASCULAR RESEARCH,2005,69(1-2):64-70.
APA Zhang, SH.,Lu, S.,Ge, JB.,Guo, JX.,Chen, P.,...&Li, LS.(2005).Increased heme oxygenase-1 expression in infarcted rat hearts following human bone marrow mesenchymal cell transplantation.MICROVASCULAR RESEARCH,69(1-2),64-70.
MLA Zhang, SH,et al."Increased heme oxygenase-1 expression in infarcted rat hearts following human bone marrow mesenchymal cell transplantation".MICROVASCULAR RESEARCH 69.1-2(2005):64-70.
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