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学科主题: 临床医学
题名:
Differentiation of Trichophyton rubrum clinical isolates from Japanese and Chinese patients by randomly amplified polymorphic DNA and DNA sequence analysis of the non-transcribed spacer region of the rRNA gene
作者: Yang, Xiumin1,2,3; Sugita, Takashi1; Takashima, Masako4; Hiruma, Masataro5; Li, Ruoyu6,7; Sudo, Hajime2; Ogawa, Hideoki2; Ikeda, Shigaku2
关键词: Trichophyton rubrum ; RAPD ; NTS ; rRNA gene ; Japan ; China
刊名: JOURNAL OF DERMATOLOGICAL SCIENCE
发表日期: 2009-04-01
DOI: 10.1016/j.jdermsci.2008.12.009
卷: 54, 期:1, 页:38-42
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dermatology
研究领域[WOS]: Dermatology
关键词[WOS]: SPECIES-IDENTIFICATION ; DERMATOPHYTES ; TONSURANS ; MENTAGROPHYTES ; HETEROGENEITY ; AMPLIFICATION ; VIOLACEUM ; DIVERSITY
英文摘要:

Background: Trichophyton rubrum is the most common pathogen causing dermatophytosis worldwide. Recent genetic investigations showed that the microorganism originated in Africa and then spread to Europe and North America via Asia.

Objects: We investigated the intraspecific diversity of T. rubrum isolated from two closely located Asian countries, Japan and China.

Methods: A total of 150 clinical isolates of T. rubrum obtained from Japanese and Chinese patients were analyzed by randomly amplified polymorphic DNA (RAPD) and DNA sequence analysis of the non-transcribed spacer (NITS) region in the rRNA gene.

Results: RAPD analysis divided the 150 strains into two major clusters, A and B. Of the Japanese isolates, 30% belonged to cluster A and 70% belonged to cluster B, whereas 91% of the Chinese isolates were in cluster A. The NTS region of the rRNA gene was divided into four major groups (I-IV) based oil DNA sequencing. The majority of Japanese isolates were type IV (51%), and the majority of Chinese isolates were type III (75%).

Conclusions: These results suggest that although Japan and China are neighboring countries, the origins of T. rubrum isolates from these countries may not be identical. These findings provide information useful for tracing the global transmission routes of T. rubrum. (C) 2008 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.

语种: 英语
项目资助者: Japan International Cooperation Agency
WOS记录号: WOS:000264351700007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53375
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

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作者单位: 1.Meiji Pharmaceut Univ, Dept Microbiol, Tokyo 2048588, Japan
2.Juntendo Univ, Sch Med, Dept Dermatol, Bunkyo Ku, Tokyo 1138421, Japan
3.Capital Univ Med Sci, Beijing Tongren Hosp, Dept Dermatol, Beijing 100730, Peoples R China
4.RIKEN BioResource Ctr, Microbe Div Japan Collect Microorganisms, Wako, Saitama 3510198, Japan
5.Juntendo Univ, Nerima Hosp, Dept Dermatol & Allergy, Nerima Ku, Tokyo 1770033, Japan
6.Peking Univ, Hosp 1, Dept Dermatol, Beijing 100034, Peoples R China
7.Peking Univ, Hosp 1, Res Ctr Med Mycol, Beijing 100034, Peoples R China

Recommended Citation:
Yang, Xiumin,Sugita, Takashi,Takashima, Masako,et al. Differentiation of Trichophyton rubrum clinical isolates from Japanese and Chinese patients by randomly amplified polymorphic DNA and DNA sequence analysis of the non-transcribed spacer region of the rRNA gene[J]. JOURNAL OF DERMATOLOGICAL SCIENCE,2009,54(1):38-42.
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