IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科
学科主题临床医学
Differentiation of Trichophyton rubrum clinical isolates from Japanese and Chinese patients by randomly amplified polymorphic DNA and DNA sequence analysis of the non-transcribed spacer region of the rRNA gene
Yang, Xiumin1,2,3; Sugita, Takashi1; Takashima, Masako4; Hiruma, Masataro5; Li, Ruoyu6,7; Sudo, Hajime2; Ogawa, Hideoki2; Ikeda, Shigaku2
关键词Trichophyton Rubrum Rapd Nts Rrna Gene Japan China
刊名JOURNAL OF DERMATOLOGICAL SCIENCE
2009-04-01
DOI10.1016/j.jdermsci.2008.12.009
54期:1页:38-42
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dermatology
研究领域[WOS]Dermatology
关键词[WOS]SPECIES-IDENTIFICATION ; DERMATOPHYTES ; TONSURANS ; MENTAGROPHYTES ; HETEROGENEITY ; AMPLIFICATION ; VIOLACEUM ; DIVERSITY
英文摘要

Background: Trichophyton rubrum is the most common pathogen causing dermatophytosis worldwide. Recent genetic investigations showed that the microorganism originated in Africa and then spread to Europe and North America via Asia.

Objects: We investigated the intraspecific diversity of T. rubrum isolated from two closely located Asian countries, Japan and China.

Methods: A total of 150 clinical isolates of T. rubrum obtained from Japanese and Chinese patients were analyzed by randomly amplified polymorphic DNA (RAPD) and DNA sequence analysis of the non-transcribed spacer (NITS) region in the rRNA gene.

Results: RAPD analysis divided the 150 strains into two major clusters, A and B. Of the Japanese isolates, 30% belonged to cluster A and 70% belonged to cluster B, whereas 91% of the Chinese isolates were in cluster A. The NTS region of the rRNA gene was divided into four major groups (I-IV) based oil DNA sequencing. The majority of Japanese isolates were type IV (51%), and the majority of Chinese isolates were type III (75%).

Conclusions: These results suggest that although Japan and China are neighboring countries, the origins of T. rubrum isolates from these countries may not be identical. These findings provide information useful for tracing the global transmission routes of T. rubrum. (C) 2008 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000264351700007
资助机构Japan International Cooperation Agency
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53375
专题北京大学第一临床医学院_皮肤性病科
作者单位1.Meiji Pharmaceut Univ, Dept Microbiol, Tokyo 2048588, Japan
2.Juntendo Univ, Sch Med, Dept Dermatol, Bunkyo Ku, Tokyo 1138421, Japan
3.Capital Univ Med Sci, Beijing Tongren Hosp, Dept Dermatol, Beijing 100730, Peoples R China
4.RIKEN BioResource Ctr, Microbe Div Japan Collect Microorganisms, Wako, Saitama 3510198, Japan
5.Juntendo Univ, Nerima Hosp, Dept Dermatol & Allergy, Nerima Ku, Tokyo 1770033, Japan
6.Peking Univ, Hosp 1, Dept Dermatol, Beijing 100034, Peoples R China
7.Peking Univ, Hosp 1, Res Ctr Med Mycol, Beijing 100034, Peoples R China
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GB/T 7714
Yang, Xiumin,Sugita, Takashi,Takashima, Masako,et al. Differentiation of Trichophyton rubrum clinical isolates from Japanese and Chinese patients by randomly amplified polymorphic DNA and DNA sequence analysis of the non-transcribed spacer region of the rRNA gene[J]. JOURNAL OF DERMATOLOGICAL SCIENCE,2009,54(1):38-42.
APA Yang, Xiumin.,Sugita, Takashi.,Takashima, Masako.,Hiruma, Masataro.,Li, Ruoyu.,...&Ikeda, Shigaku.(2009).Differentiation of Trichophyton rubrum clinical isolates from Japanese and Chinese patients by randomly amplified polymorphic DNA and DNA sequence analysis of the non-transcribed spacer region of the rRNA gene.JOURNAL OF DERMATOLOGICAL SCIENCE,54(1),38-42.
MLA Yang, Xiumin,et al."Differentiation of Trichophyton rubrum clinical isolates from Japanese and Chinese patients by randomly amplified polymorphic DNA and DNA sequence analysis of the non-transcribed spacer region of the rRNA gene".JOURNAL OF DERMATOLOGICAL SCIENCE 54.1(2009):38-42.
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