学科主题基础医学
The effect of surface agitation on ultrasound-mediated gene transfer in vitro
Chen, WS1; Lu, XC1; Liu, YB1; Zhong, P1
刊名JOURNAL OF THE ACOUSTICAL SOCIETY OF AMERICA
2004-10-01
DOI10.1121/1.1777855
116期:4页:2440-2450
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Acoustics ; Audiology & Speech-Language Pathology
研究领域[WOS]Acoustics ; Audiology & Speech-Language Pathology
关键词[WOS]SEVERE COMBINED IMMUNODEFICIENCY ; WAVE-INDUCED CAVITATION ; RECTIFIED DIFFUSION ; THERAPY ; BUBBLES ; MICROBUBBLES ; DELIVERY ; CELLS ; SONOPORATION ; DESTRUCTION
英文摘要

This article reports the effect of surface agitation of culture medium on ultrasound-mediated gene transfection in vitro and its possible mechanisms. The possibility of active induction of bubbles without using contrast microbubbles for effective gene transfer was also demonstrated. Cultured HeLa cells mixed with green fluorescent protein plasmid were exposed to 1.0 MHz ultrasound in 24-well culture plates. Up to 26% transfection efficiency in the survival cell population was achieved in samples exposed to 0.44 MPa ultrasound pulses with the presence of surface agitation. Inertial cavitation and bubble generation were observed throughout the ultrasound exposure. When surface agitation was suppressed by covering the medium surface with a thin membrane, bubble generation and gene transfection were significantly suppressed. Interestingly, transfection efficiency could be partially resumed by adding a small amount of culture medium onto the covering membrane to rebuild the surface agitation and bubble generation. Pressure fluctuation and transient high-pressure loci were found in samples with surface agitation. Numerical simulations of bubble dynamics showed that transient high pressures above the inertial cavitation threshold could generate bubbles, which might be subsequently stabilized at lower pressures by rectified diffusion, and exert strong shear forces that might create transient pores on cell membranes to facilitate gene transfer. (C) 2004 Acoustical Society of America.

语种英语
WOS记录号WOS:000224655600058
引用统计
被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53377
专题北京大学基础医学院_生物化学与分子生物学系
作者单位1.Natl Taiwan Univ, Coll Med, Taipei 100, Taiwan
2.Duke Univ, Dept Mech Engn & Mat Sci, Durham, NC 27708 USA
3.Natl Taiwan Univ Hosp, Dept Phys Med & Rehabil, Taipei 100, Taiwan
4.Peking Univ, Med Ctr, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
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GB/T 7714
Chen, WS,Lu, XC,Liu, YB,et al. The effect of surface agitation on ultrasound-mediated gene transfer in vitro[J]. JOURNAL OF THE ACOUSTICAL SOCIETY OF AMERICA,2004,116(4):2440-2450.
APA Chen, WS,Lu, XC,Liu, YB,&Zhong, P.(2004).The effect of surface agitation on ultrasound-mediated gene transfer in vitro.JOURNAL OF THE ACOUSTICAL SOCIETY OF AMERICA,116(4),2440-2450.
MLA Chen, WS,et al."The effect of surface agitation on ultrasound-mediated gene transfer in vitro".JOURNAL OF THE ACOUSTICAL SOCIETY OF AMERICA 116.4(2004):2440-2450.
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