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学科主题: 基础医学
题名:
The effect of surface agitation on ultrasound-mediated gene transfer in vitro
作者: Chen, WS1; Lu, XC1; Liu, YB1; Zhong, P1
刊名: JOURNAL OF THE ACOUSTICAL SOCIETY OF AMERICA
发表日期: 2004-10-01
DOI: 10.1121/1.1777855
卷: 116, 期:4, 页:2440-2450
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Acoustics ; Audiology & Speech-Language Pathology
研究领域[WOS]: Acoustics ; Audiology & Speech-Language Pathology
关键词[WOS]: SEVERE COMBINED IMMUNODEFICIENCY ; WAVE-INDUCED CAVITATION ; RECTIFIED DIFFUSION ; THERAPY ; BUBBLES ; MICROBUBBLES ; DELIVERY ; CELLS ; SONOPORATION ; DESTRUCTION
英文摘要:

This article reports the effect of surface agitation of culture medium on ultrasound-mediated gene transfection in vitro and its possible mechanisms. The possibility of active induction of bubbles without using contrast microbubbles for effective gene transfer was also demonstrated. Cultured HeLa cells mixed with green fluorescent protein plasmid were exposed to 1.0 MHz ultrasound in 24-well culture plates. Up to 26% transfection efficiency in the survival cell population was achieved in samples exposed to 0.44 MPa ultrasound pulses with the presence of surface agitation. Inertial cavitation and bubble generation were observed throughout the ultrasound exposure. When surface agitation was suppressed by covering the medium surface with a thin membrane, bubble generation and gene transfection were significantly suppressed. Interestingly, transfection efficiency could be partially resumed by adding a small amount of culture medium onto the covering membrane to rebuild the surface agitation and bubble generation. Pressure fluctuation and transient high-pressure loci were found in samples with surface agitation. Numerical simulations of bubble dynamics showed that transient high pressures above the inertial cavitation threshold could generate bubbles, which might be subsequently stabilized at lower pressures by rectified diffusion, and exert strong shear forces that might create transient pores on cell membranes to facilitate gene transfer. (C) 2004 Acoustical Society of America.

语种: 英语
WOS记录号: WOS:000224655600058
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53377
Appears in Collections:基础医学院_生物化学与分子生物学系_期刊论文

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作者单位: 1.Natl Taiwan Univ, Coll Med, Taipei 100, Taiwan
2.Duke Univ, Dept Mech Engn & Mat Sci, Durham, NC 27708 USA
3.Natl Taiwan Univ Hosp, Dept Phys Med & Rehabil, Taipei 100, Taiwan
4.Peking Univ, Med Ctr, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China

Recommended Citation:
Chen, WS,Lu, XC,Liu, YB,et al. The effect of surface agitation on ultrasound-mediated gene transfer in vitro[J]. JOURNAL OF THE ACOUSTICAL SOCIETY OF AMERICA,2004,116(4):2440-2450.
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