北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 免疫学系  > 期刊论文
学科主题: 基础医学
题名:
Anti-T-cell humoral and cellular responses in healthy BALB/c mice following immunization with ovalbumin or ovalbumin-specific T cells
作者: Zhang, XY; Liu, XG; Wang, W; Wang, WC; Gao, XM
刊名: IMMUNOLOGY
发表日期: 2003-04-01
卷: 108, 期:4, 页:465-473
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: MYELIN-BASIC-PROTEIN ; EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS ; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS ; RECEPTOR BETA-CHAIN ; MULTIPLE-SCLEROSIS ; PILOT TRIAL ; VACCINATION ; MECHANISMS ; THERAPY ; TOLERANCE
英文摘要:

It is known that T-cell vaccination (TCV) elicits cellular immune responses against the immunizing T cells in vivo . However, it is still unclear whether similar anti-T-cell responses are also induced in individuals responding to foreign antigen (Ag) challenge. This study was undertaken to characterize and compare anti-T-cell cellular and humoral responses of BALB/c mice after ovalbumin (OVA) immunization or TCV. Splenocytes from OVA-primed BALB/c mice proliferated in response to stimulation with a syngeneic OVA-specific T-cell clone, OVA-T3, and secreted interferon-gamma (IFN-gamma) but not interleukin-4 (IL-4). Cytotoxic T-lymphocyte (CTL) activity against the T-cell clone was also observed. Serum samples from these animals discriminated between activated and resting murine T cells, as determined by indirect immunofluorescence staining. Vaccination of BALB/c mice with OVA-T3 cells induced similar, but stronger, cellular and humoral responses. TCV-induced antibodies were not only able to distinguish between activated and resting syngeneic T cells but also positively stained allogeneic T cells from BXSB mice. Furthermore, TCV resulted in hyporesponsiveness of BALB/c mice to subsequent Ag challenge, and antisera from the immunized animals inhibited T-cell proliferation in vitro. Our data suggest that anti-T-cell antibodies, and CD4(+) and CD8(+) regulatory T lymphocytes may form a complex and co-ordinated regulatory network that plays an important role in regulating the adaptive immune responses in vivo . Implications of this observation for our understanding of the immunoregulation and peripheral tolerance are discussed.

语种: 英语
WOS记录号: WOS:000181966900006
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53392
Appears in Collections:基础医学院_免疫学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Zhang_et_al-2003-Immunology.pdf(262KB)期刊论文出版稿限制开放 联系获取全文

作者单位: Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China

Recommended Citation:
Zhang, XY,Liu, XG,Wang, W,et al. Anti-T-cell humoral and cellular responses in healthy BALB/c mice following immunization with ovalbumin or ovalbumin-specific T cells[J]. IMMUNOLOGY,2003,108(4):465-473.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, XY]'s Articles
[Liu, XG]'s Articles
[Wang, W]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, XY]‘s Articles
[Liu, XG]‘s Articles
[Wang, W]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace