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学科主题: 基础医学
题名:
SMAD3 regulates the diverse functions of rat granulosa cells relating to the FSHR/PKA signaling pathway
作者: Li, Yexia; Jin, Yujie; Liu, Yuxia; Shen, Chunyan; Dong, Jingxia; Xu, Jian
刊名: REPRODUCTION
发表日期: 2013-08-01
DOI: 10.1530/REP-12-0325
卷: 146, 期:2, 页:169-179
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Developmental Biology ; Reproductive Biology
研究领域[WOS]: Developmental Biology ; Reproductive Biology
关键词[WOS]: GROWTH-FACTOR-BETA ; OVARIAN FOLLICULAR DEVELOPMENT ; HAMSTER PREANTRAL FOLLICLES ; MESSENGER-RIBONUCLEIC-ACID ; DNA-SYNTHESIS ; IMMATURE MICE ; ACTIVIN-A ; SUPERFAMILY ; EXPRESSION ; DIFFERENTIATION
英文摘要:

The function of Smad3, a downstream signaling protein of the transforming growth factor beta (TGF beta) pathway, in ovarian follicle development remains to be elucidated. The effects of Smad3 on ovarian granulosa cells (GCs) in rat were studied. Female rats (21 days of age Sprague-Dawley) received i.p. injections of pregnant mare serum gonadotropin, and GCs were harvested for primary culture 48 h later. These cells were engineered to overexpress or knockdown Smad3, which were validated by immunohistochemistry and western blot. The expression of proliferating cell nuclear antigen (PCNA), cyclin D2, TGF beta receptor II (TGF beta RII), protein kinase A (PKA), and FSH receptor (FSHR) was also detected by western blotting. Cell cycle and apoptosis of GCs were assayed by flow cytometry. The level of estrogen secreted by GCs was detected by ELISA. Smad3 overexpression promoted estrogen production and proliferation while inhibiting apoptosis of GCs. Reduction in Smad3 by RNAi resulted in reduced estrogen production and proliferation and increased apoptosis of GCs. Manipulation of Smad3 expression also resulted in changes in FSHR and PKA expression, suggesting that the effects of Smad3 on follicle development are related to FSHR-mediated cAMP signaling.

语种: 英语
所属项目编号: 30772344
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000322366900011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53395
Appears in Collections:基础医学院_期刊论文

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作者单位: Peking Univ, Dept Anat Histol & Embryol, Sch Basic Med Sci, Beijing 100191, Peoples R China

Recommended Citation:
Li, Yexia,Jin, Yujie,Liu, Yuxia,et al. SMAD3 regulates the diverse functions of rat granulosa cells relating to the FSHR/PKA signaling pathway[J]. REPRODUCTION,2013,146(2):169-179.
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