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SMAD3 regulates the diverse functions of rat granulosa cells relating to the FSHR/PKA signaling pathway
Li, Yexia; Jin, Yujie; Liu, Yuxia; Shen, Chunyan; Dong, Jingxia; Xu, Jian
刊名REPRODUCTION
2013-08-01
DOI10.1530/REP-12-0325
146期:2页:169-179
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Developmental Biology ; Reproductive Biology
研究领域[WOS]Developmental Biology ; Reproductive Biology
关键词[WOS]GROWTH-FACTOR-BETA ; OVARIAN FOLLICULAR DEVELOPMENT ; HAMSTER PREANTRAL FOLLICLES ; MESSENGER-RIBONUCLEIC-ACID ; DNA-SYNTHESIS ; IMMATURE MICE ; ACTIVIN-A ; SUPERFAMILY ; EXPRESSION ; DIFFERENTIATION
英文摘要

The function of Smad3, a downstream signaling protein of the transforming growth factor beta (TGF beta) pathway, in ovarian follicle development remains to be elucidated. The effects of Smad3 on ovarian granulosa cells (GCs) in rat were studied. Female rats (21 days of age Sprague-Dawley) received i.p. injections of pregnant mare serum gonadotropin, and GCs were harvested for primary culture 48 h later. These cells were engineered to overexpress or knockdown Smad3, which were validated by immunohistochemistry and western blot. The expression of proliferating cell nuclear antigen (PCNA), cyclin D2, TGF beta receptor II (TGF beta RII), protein kinase A (PKA), and FSH receptor (FSHR) was also detected by western blotting. Cell cycle and apoptosis of GCs were assayed by flow cytometry. The level of estrogen secreted by GCs was detected by ELISA. Smad3 overexpression promoted estrogen production and proliferation while inhibiting apoptosis of GCs. Reduction in Smad3 by RNAi resulted in reduced estrogen production and proliferation and increased apoptosis of GCs. Manipulation of Smad3 expression also resulted in changes in FSHR and PKA expression, suggesting that the effects of Smad3 on follicle development are related to FSHR-mediated cAMP signaling.

语种英语
WOS记录号WOS:000322366900011
项目编号30772344
资助机构National Natural Science Foundation of China
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53395
专题北京大学基础医学院
作者单位Peking Univ, Dept Anat Histol & Embryol, Sch Basic Med Sci, Beijing 100191, Peoples R China
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GB/T 7714
Li, Yexia,Jin, Yujie,Liu, Yuxia,et al. SMAD3 regulates the diverse functions of rat granulosa cells relating to the FSHR/PKA signaling pathway[J]. REPRODUCTION,2013,146(2):169-179.
APA Li, Yexia,Jin, Yujie,Liu, Yuxia,Shen, Chunyan,Dong, Jingxia,&Xu, Jian.(2013).SMAD3 regulates the diverse functions of rat granulosa cells relating to the FSHR/PKA signaling pathway.REPRODUCTION,146(2),169-179.
MLA Li, Yexia,et al."SMAD3 regulates the diverse functions of rat granulosa cells relating to the FSHR/PKA signaling pathway".REPRODUCTION 146.2(2013):169-179.
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