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Identification of tanshinones and their metabolites in rat bile after oral administration of TTE-50, a standardized extract of Salvia miltiorrhiza by HPLG-ESI-DAD-MSn
Sun, Hang-Hao; Yang, Min; Wang, Xiao-Ming; Xu, Man; Liu, Ai-Hua; Guo, De-An
关键词High Performance Liquid Chromatography-tandem Mass Spectrometry Tanshinone Metabolism Rat Bile
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2007-06-28
DOI10.1016/j.jpba.2006.11.003
44期:2页:564-574
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]TANDEM MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; CHINESE MEDICINAL PREPARATIONS ; PRESSURE CHEMICAL-IONIZATION ; IN-VITRO CYTOTOXICITY ; IIA ; CRYPTOTANSHINONE ; ELECTROSPRAY ; PLASMA ; CELLS
英文摘要

TTE-50 is a standardized extract of Salvia miltiorrhiza which mainly consisted of tanshinones. A sensitive and specific method using liquid chromatography-diode array detection-electrospray ionization (ESI) ion trap mass spectrometry was established for the study of the constituents and metabolites of TTE-50 in rat bile sample after oral administration. The bile samples were extracted with ethyl acetate (EtOAc) of three-fold volume for three times. The chromatographic separation was carried out on a Zorbax Extend-C-18 column with a gradient elution program whereas acetonitrile-water was used as mobile phase. Mass spectra were acquired in positive ionization mode and data-dependant scan was used for the identification of the tanshinones and metabolites in the bile samples. Identification and structural elucidation of the tanshinones and their metabolites in bile samples were performed by comparing their retention-times and full scan MSn spectra with those of reference compounds and data in the literatures. Sixteen tanshinones in TTE-50 along with seventeen phase I metabolites were identified simultaneously. The metabolic modification could take place in the C-4 side chain of tanshinone IIA, from methyl to primary alcohol, then to aldehyde group was proposed for the first time. The established method was valuable for the study of the metabolism of complex system such as herbal extracts or traditional Chinese medicine (TCM) formula. (c) 2006 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000247765400032
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53397
专题北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
2.Chinese Acad Sci, Shanghai Res Ctr TCM Modernizat, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Shanghai 201203, Peoples R China
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GB/T 7714
Sun, Hang-Hao,Yang, Min,Wang, Xiao-Ming,et al. Identification of tanshinones and their metabolites in rat bile after oral administration of TTE-50, a standardized extract of Salvia miltiorrhiza by HPLG-ESI-DAD-MSn[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2007,44(2):564-574.
APA Sun, Hang-Hao,Yang, Min,Wang, Xiao-Ming,Xu, Man,Liu, Ai-Hua,&Guo, De-An.(2007).Identification of tanshinones and their metabolites in rat bile after oral administration of TTE-50, a standardized extract of Salvia miltiorrhiza by HPLG-ESI-DAD-MSn.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,44(2),564-574.
MLA Sun, Hang-Hao,et al."Identification of tanshinones and their metabolites in rat bile after oral administration of TTE-50, a standardized extract of Salvia miltiorrhiza by HPLG-ESI-DAD-MSn".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 44.2(2007):564-574.
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