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学科主题: 基础医学
题名:
Comparison of Three Embryo Culture Methods for Derivation of Human Embryonic Stem Cells from Discarded Embryos
作者: Liu, Ying1; Li, Yang2; Hwang, Andrew3; Wang, Shu-yu1; Jia, Chan-wei1; Yu, Lan; Li, Jian1
刊名: CELLULAR REPROGRAMMING
发表日期: 2011-06-01
DOI: 10.1089/cell.2010.0092
卷: 13, 期:3, 页:233-239
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Genetics & Heredity
研究领域[WOS]: Cell Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
关键词[WOS]: CLEAVAGE-STAGE EMBRYOS ; POOR-QUALITY EMBRYOS ; IN-VITRO ; HUMAN BLASTOCYSTS ; LINES
英文摘要:

Human embryonic stem cells (hESC) are self-renewing and pluripotent cells that hold great promise. Our objective was to compare the effect of three different embryo culture methods for derivation of human embryonic stem cells from discarded embryos. A prospective and randomized trial was conducted using 381 discarded human embryos at days 2-3 postfertilization in Beijing Obstetrics and Gynecology Hospital IVF center. After removal of the zona pellucida, discarded human embryos were cultured by three different methods as multiple embryo aggregates, single embryo, and blastomeres. Outgrowth of embryos and hESC derivation were observed. The outgrowth rate of embryos cultured as multiple embryo aggregates was higher than that of those cultured as single embryos or blastomeres (p < 0.05). Three propagating hESC lines were derived from poor quality day 2-3 postfertilization nonblastocyst embryos cultured as multiple embryo aggregates. Derived hESC lines expressed hESC-specific markers of pluripotency and had normal diploid karyotype. The cells were able to form derivatives of all three germ layers in vivo as teratomas. Our results demonstrate that culturing these discarded embryos as multiple embryo aggregates was more profitable for outgrowth and derivation of ESC line than culturing these as single embryo or blastomeres.

语种: 英语
所属项目编号: H020821200190 ; 20042B0301037
项目资助者: Beijing New Star Technology Project ; Beijing Collective Talents Project ; Beijing Tens-Hundreds-Thousands of Health Personnel Project
WOS记录号: WOS:000291205400005
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53398
Appears in Collections:基础医学院_北京大学干细胞研究中心_期刊论文

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作者单位: 1.Capital Univ Med Sci, Beijing Obstet & Gynecol Hosp, Dept Reprod Med, Beijing, Peoples R China
2.Peking Univ, Stem Cell Res Ctr, Hlth Sci Ctr, Beijing 100871, Peoples R China
3.Stanford Univ, Dept Radiol, Sch Med, Stanford, CA 94305 USA

Recommended Citation:
Liu, Ying,Li, Yang,Hwang, Andrew,et al. Comparison of Three Embryo Culture Methods for Derivation of Human Embryonic Stem Cells from Discarded Embryos[J]. CELLULAR REPROGRAMMING,2011,13(3):233-239.
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