学科主题基础医学
Comparison of Three Embryo Culture Methods for Derivation of Human Embryonic Stem Cells from Discarded Embryos
Liu, Ying1; Li, Yang2; Hwang, Andrew3; Wang, Shu-yu1; Jia, Chan-wei1; Yu, Lan; Li, Jian1
刊名CELLULAR REPROGRAMMING
2011-06-01
DOI10.1089/cell.2010.0092
13期:3页:233-239
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering ; Biotechnology & Applied Microbiology ; Genetics & Heredity
研究领域[WOS]Cell Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
关键词[WOS]CLEAVAGE-STAGE EMBRYOS ; POOR-QUALITY EMBRYOS ; IN-VITRO ; HUMAN BLASTOCYSTS ; LINES
英文摘要

Human embryonic stem cells (hESC) are self-renewing and pluripotent cells that hold great promise. Our objective was to compare the effect of three different embryo culture methods for derivation of human embryonic stem cells from discarded embryos. A prospective and randomized trial was conducted using 381 discarded human embryos at days 2-3 postfertilization in Beijing Obstetrics and Gynecology Hospital IVF center. After removal of the zona pellucida, discarded human embryos were cultured by three different methods as multiple embryo aggregates, single embryo, and blastomeres. Outgrowth of embryos and hESC derivation were observed. The outgrowth rate of embryos cultured as multiple embryo aggregates was higher than that of those cultured as single embryos or blastomeres (p < 0.05). Three propagating hESC lines were derived from poor quality day 2-3 postfertilization nonblastocyst embryos cultured as multiple embryo aggregates. Derived hESC lines expressed hESC-specific markers of pluripotency and had normal diploid karyotype. The cells were able to form derivatives of all three germ layers in vivo as teratomas. Our results demonstrate that culturing these discarded embryos as multiple embryo aggregates was more profitable for outgrowth and derivation of ESC line than culturing these as single embryo or blastomeres.

语种英语
WOS记录号WOS:000291205400005
项目编号H020821200190 ; 20042B0301037
资助机构Beijing New Star Technology Project ; Beijing Collective Talents Project ; Beijing Tens-Hundreds-Thousands of Health Personnel Project
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53398
专题北京大学基础医学院_北京大学干细胞研究中心
作者单位1.Capital Univ Med Sci, Beijing Obstet & Gynecol Hosp, Dept Reprod Med, Beijing, Peoples R China
2.Peking Univ, Stem Cell Res Ctr, Hlth Sci Ctr, Beijing 100871, Peoples R China
3.Stanford Univ, Dept Radiol, Sch Med, Stanford, CA 94305 USA
推荐引用方式
GB/T 7714
Liu, Ying,Li, Yang,Hwang, Andrew,et al. Comparison of Three Embryo Culture Methods for Derivation of Human Embryonic Stem Cells from Discarded Embryos[J]. CELLULAR REPROGRAMMING,2011,13(3):233-239.
APA Liu, Ying.,Li, Yang.,Hwang, Andrew.,Wang, Shu-yu.,Jia, Chan-wei.,...&Li, Jian.(2011).Comparison of Three Embryo Culture Methods for Derivation of Human Embryonic Stem Cells from Discarded Embryos.CELLULAR REPROGRAMMING,13(3),233-239.
MLA Liu, Ying,et al."Comparison of Three Embryo Culture Methods for Derivation of Human Embryonic Stem Cells from Discarded Embryos".CELLULAR REPROGRAMMING 13.3(2011):233-239.
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