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学科主题: 临床医学
题名:
Comparison of tolerated and rejected islet grafts: A gene expression study
作者: Berg, T; Wu, T; Levay-Young, B; Heuss, N; Pan, YS; Kirchhof, N; Sutherland, DER; Hering, BJ; Guo, ZG
关键词: gene expression ; islets ; NOD mice ; rejection ; tolerance ; graft-infiltrating lymphocytes
刊名: CELL TRANSPLANTATION
发表日期: 2004
卷: 13, 期:6, 页:619-629
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Medicine, Research & Experimental ; Transplantation
研究领域[WOS]: Cell Biology ; Research & Experimental Medicine ; Transplantation
关键词[WOS]: ACUTE ALLOGRAFT-REJECTION ; TRANSPLANT REJECTION ; DNA MICROARRAY ; IFN-GAMMA ; T-CELLS ; ACTIVATION TRANSCRIPTS ; CELLULAR INFILTRATION ; CDNA MICROARRAYS ; RECEPTOR ; RAT
英文摘要:

Recently we showed that donor-specific tolerance to MHC-matched islet allografts in diabetic NOD mice could be induced by simultaneous islet and bone marrow transplantation. Mononuclear cell infiltration surrounding the islets was also found in tolerated grafts. In this study, we compared gene expression in the tolerated and rejected islet grafts by using Affymetrix Murine U74A oligonucleotide arrays. To confirm the results of microarray analysis, we performed real-time PCR and RNase protection assay on selected genes. Of over 12,000 genes studied, 57 genes were expressed at consistently higher levels in tolerated islet grafts, and 524 genes in rejected islet grafts. Genes from a variety of functional clusters were found to be different between rejected and tolerated grafts. In the rejected islet grafts, a number of T-cell surface markers and of cytotoxicity-related genes were highly expressed. Also in the rejected grafts, a number of cytokines and chemokines and their receptors were highly expressed. The differential expression of selected genes found by microarray analysis was also confirmed by real-time PCR and RNase protection assay. Our results indicated that gene microarray analysis can help us to detect gene expression differences representative of the biologic mechanisms of tolerance and rejection.

语种: 英语
WOS记录号: WOS:000225813500002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53401
Appears in Collections:北京大学第一临床医学院_普通外科_期刊论文

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作者单位: 1.Univ Minnesota, Sch Med, Islet Transplant Program, Diabet Inst Immunol & Transplantat, Minneapolis, MN 55455 USA
2.Univ Minnesota, Dept Surg, Minneapolis, MN 55455 USA
3.Univ Freiburg Klinikum, Freiburg, Germany
4.Beijing Univ, Hosp 1, Dept Surg, Beijing 100871, Peoples R China

Recommended Citation:
Berg, T,Wu, T,Levay-Young, B,et al. Comparison of tolerated and rejected islet grafts: A gene expression study[J]. CELL TRANSPLANTATION,2004,13(6):619-629.
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