IR@PKUHSC  > 北京大学第一临床医学院  > 肾脏内科
学科主题临床医学
Identification of adipocyte adhesion molecule (ACAM), a novel CTX gene family, implicated in adipocyte maturation and development of obesity
Eguchi, J; Wada, J; Hida, K; Zhang, H; Matsuoka, T; Baba, M; Hashimoto, I; Shikata, K; Ogawa, N; Makino, H
关键词Adipose Tissue Adhesion Molecule Cell Aggregation Ctx Gene Family Obesity
刊名BIOCHEMICAL JOURNAL
2005-04-15
DOI10.1042/BJ20041709
387页:343-353
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]IMMUNOGLOBULIN SUPERFAMILY ; TRANSMEMBRANE GLYCOPROTEIN ; ADENOVIRUS RECEPTOR ; ENDOTHELIAL-CELLS ; TIGHT JUNCTION ; A33 ANTIGEN ; MEMBRANE ; DIFFERENTIATION ; CLONING ; MEMBER
英文摘要

Few cell adhesion molecules have been reported to be expressed in mature adipocytes, and the significance of cell adhesion process in adipocyte biology is also unknown. In the present study, we identified ACAM (adipocyte adhesion molecule), a novel homologue of the CTX (cortical thymocyte marker in Xenopus) gene family. ACAM cDNA was isolated during PCR-based cDNA subtraction, and its mRNA was shown to be up-regulated in WATs (white adipose tissues) of OLETF (Otsuka Long-Evans Tokushima fatty) rats, an animal model for Type II diabetes and obesity. ACAM, 372 amino acids in total, has a signal peptide, V-type (variable) and C2-type (constant) Ig domains, a single transmembrane segment and a cytoplasmic tail. The amino acid sequence in rat is highly homologous to mouse (94%) and human (87%). ACAM mRNA was predominantly expressed in WATs in OLETF rats, and increased with the development of obesity until 30 weeks of age, which is when the peak of body mass is reached. Western blot analysis revealed that ACAM protein, approx. 45 kDa, was associated with plasma membrane fractions of mature adipocytes isolated from mesenteric and subdermal adipose deposits of OLETF rats. Up-regulation of ACAM mRNAs in obesity was also shown in WATs of genetically obese db/db mice, diet-induced obese ICR mice and human obese subjects. In primary cultured mouse and human adipocytes, ACAM mRNA expression was progressively up-regulated during differentiation. Several stably transfected Chinese-hamster ovary K1 cell lines were established, and the quantification of ACAM mRNA and cell aggregation assay revealed that the degree of homophilic aggregation correlated well with ACAM mRNA expression. In summary, ACAM may be the critical adhesion molecule in adipocyte differentiation and development of obesity.

语种英语
WOS记录号WOS:000228699200007
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被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53403
专题北京大学第一临床医学院_肾脏内科
作者单位1.Okayama Univ, Grad Sch Med & Dent, Dept Med & Clin Sci, Okayama 7008558, Japan
2.Beijing Med Univ, Inst Nephrol, Teaching Hosp 1, Beijing 100034, Peoples R China
3.Okayama Univ, Grad Sch Med & Dent, Dept Brain Sci, Okayama 7008558, Japan
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Eguchi, J,Wada, J,Hida, K,et al. Identification of adipocyte adhesion molecule (ACAM), a novel CTX gene family, implicated in adipocyte maturation and development of obesity[J]. BIOCHEMICAL JOURNAL,2005,387:343-353.
APA Eguchi, J.,Wada, J.,Hida, K.,Zhang, H.,Matsuoka, T.,...&Makino, H.(2005).Identification of adipocyte adhesion molecule (ACAM), a novel CTX gene family, implicated in adipocyte maturation and development of obesity.BIOCHEMICAL JOURNAL,387,343-353.
MLA Eguchi, J,et al."Identification of adipocyte adhesion molecule (ACAM), a novel CTX gene family, implicated in adipocyte maturation and development of obesity".BIOCHEMICAL JOURNAL 387(2005):343-353.
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