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Mesoderm is committed to hemato-endothelial and cardiac lineages in human embryoid bodies by sequential exposure to cytokines
Zhu, Ming-Xia1,3; Zhao, Jin-Yuan1,3; Chen, Gui-An2,3
关键词Human Embryoid Bodies Mesoderm Hematopoietic-endothelial Precursors Cardiac Precursors Cytokines
刊名EXPERIMENTAL CELL RESEARCH
2013
DOI10.1016/j.yexcr.2012.09.009
319期:1页:21-34
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Cell Biology
研究领域[WOS]Oncology ; Cell Biology
关键词[WOS]STEM-CELL DIFFERENTIATION ; HEMATOPOIETIC DIFFERENTIATION ; PRIMITIVE STREAK ; FLK1 EXPRESSION ; MOUSE EMBRYO ; PROGENITORS ; INDUCTION ; FATE ; VEGF ; CARDIOMYOCYTES
英文摘要

Human embryonic stem (hES) cells can differentiate into cells of the three germ layers in vitro and serve as a powerful resource to study mechanisms involved in cell fate decisions. However, it is difficult to promote the directed and efficient differentiation of hES cells toward a specific lineage. Here we establish a stepwise strategy for generating hemato-endothelial and cardiac precursors from hES cells in single culture conditions. The efficiency of committing hES cells to three cell lineages was significantly higher with our approach than with exposure to single or multiple cytokines. Efficiency was determined using quantitative analysis by gene expression, flow cytometry, and colony assays. Several cytokines were sufficient to drive the efficient differentiation of hES cells into specific lineages. Each of these factors appeared to regulate specific steps of differentiation: BMP4 promoted the efficient formation of mesoderm; bFGF induced the differentiation of these mesodermal precursors to the hemangioblast fate; VEGF and TPO were required for the production of committed hematopoietic progenitors. This stepwise control of differentiation in vitro leads to a high frequency of hemato-endothelial and cardiac precursors derived from hES cells and offers a unique model for studying the molecular and cellular events that regulate hematopoiesis and cardiogenesis. (C) 2012 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000311712900003
项目编号81072256
资助机构National Natural Sciences Foundation of China
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53410
专题北京大学第三临床医学院
北京大学第三临床医学院_血液内科
作者单位1.Peking Univ, Hosp 3, Occupat Dis Res Ctr, Beijing, Peoples R China
2.Peking Univ, Hosp 3, Reprod Med Ctr, Beijing, Peoples R China
3.Peking Univ, Hosp 3, Clin Stem Cell Res Ctr, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Zhu, Ming-Xia,Zhao, Jin-Yuan,Chen, Gui-An. Mesoderm is committed to hemato-endothelial and cardiac lineages in human embryoid bodies by sequential exposure to cytokines[J]. EXPERIMENTAL CELL RESEARCH,2013,319(1):21-34.
APA Zhu, Ming-Xia,Zhao, Jin-Yuan,&Chen, Gui-An.(2013).Mesoderm is committed to hemato-endothelial and cardiac lineages in human embryoid bodies by sequential exposure to cytokines.EXPERIMENTAL CELL RESEARCH,319(1),21-34.
MLA Zhu, Ming-Xia,et al."Mesoderm is committed to hemato-endothelial and cardiac lineages in human embryoid bodies by sequential exposure to cytokines".EXPERIMENTAL CELL RESEARCH 319.1(2013):21-34.
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