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学科主题: 口腔医学
题名:
miR-29b suppresses proliferation, migration, and invasion of tongue squamous cell carcinoma through PTEN-AKT signaling pathway by targeting Sp1
作者: Jia, Ling-Fei1,2; Huang, Yi-Ping3; Zheng, Yun-Fei1; Lyu, Ming-Yue1; Wei, Su-Bi4; Meng, Zhen1; Gan, Ye-Hua1,2
关键词: Oral cancer ; Tongue squamous cell carcinoma ; miR-29b ; Sp1 ; PTEN ; AKT
刊名: ORAL ONCOLOGY
发表日期: 2014-11-01
DOI: 10.1016/j.oraloncology.2014.07.010
卷: 50, 期:11, 页:1062-1071
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Oncology ; Dentistry, Oral Surgery & Medicine
关键词[WOS]: TRANSCRIPTION FACTOR SP1 ; MULTIPLE-MYELOMA CELLS ; INDUCED APOPTOSIS ; TUMOR-SUPPRESSOR ; DOWN-REGULATION ; NECK-CANCER ; EXPRESSION ; MICRORNA-29B ; HEAD ; ACTIVATION
英文摘要:

Objectives: miR-29b has been implicated in various cancers. However, the role of miR-29b in tongue squamous cell carcinoma (TSCC) remains unclear. This study aimed to investigate the role of miR-29b in TSCC progression.

Materials and methods: The expression of miR-29b was analyzed in TSCC tissues and cells. Functional studies were performed in TSCC cells. Real time-PCR, Western blot, cell proliferation, transwell, and dual luciferase reporter assays were performed according to standard procedures.

Results: miR-29b was significantly decreased in TSCC specimens and cell lines compared with corresponding normal counterparts. Overexpression of miR-29b significantly inhibited the proliferation, migration, invasion, and cell-cycle progression of TSCC cells, and promoted apoptosis. Moreover, miR-29b targeted the 30 untranslated region of the Sp1 transcript and resulted in the deregulation of Sp1. The inhibition of Sp1 by miR-29b subsequently resulted in the upregulation of PTEN, leading to a decline of phosphorylated AKT. Knockdown of Sp1 in TSCC cell lines mimicked the effects of miR-29b overexpression. In addition, the expression of miR-29b was inversely correlated with Sp1 and positively correlated with the PTEN in TSCC specimens.

Conclusion: miR-29b functions as a tumor suppressor in TSCC, and the miR-29b/Sp1/PTEN/AKT axis might represent a potential therapeutic target for TSCC intervention. (C) 2014 Elsevier Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000344066500011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53419
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Mol Biol Lab, Beijing 100081, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Dept Orthodont, Beijing 100081, Peoples R China
4.Tsinghua Univ, Med Syst Biol Res Ctr, Beijing 100084, Peoples R China

Recommended Citation:
Jia, Ling-Fei,Huang, Yi-Ping,Zheng, Yun-Fei,et al. miR-29b suppresses proliferation, migration, and invasion of tongue squamous cell carcinoma through PTEN-AKT signaling pathway by targeting Sp1[J]. ORAL ONCOLOGY,2014,50(11):1062-1071.
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