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学科主题: 基础医学
题名:
E2F1 enhances 8-Chloro-adenosine-induced G2/M arrest and apoptosis in A549 and H1299 lung cancer cells
作者: Duan, Hong-Ying1,2; Cao, Ji-Xiang1; Qi, Jun-Juan1; Wu, Guo-Sheng1; Li, Shu-Yan1; An, Guo-Shun1; Jia, Hong-Ti1,3; Cai, Wang-Wei2; Ni, Ju-Hua1
关键词: E2F1 ; 8-chloro-adenosine ; DNA double-stranded breaks ; G2/M arrest ; apoptosis
刊名: BIOCHEMISTRY-MOSCOW
发表日期: 2012-03-01
DOI: 10.1134/S0006297912030042
卷: 77, 期:3, 页:261-269
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: NUCLEOTIDE EXCISION-REPAIR ; LEUKEMIA K562 CELLS ; DNA-DAMAGE ; TRANSCRIPTION FACTORS ; IN-VIVO ; INHIBITION ; FAMILY ; GROWTH ; DEATH ; GENE
英文摘要:

The E2F1 transcription factor is a well known regulator of cell proliferation and apoptosis, but its role in response to DNA damage is less clear. 8-Chloro-adenosine (8-Cl-Ado), a nucleoside analog, can inhibit proliferation in a variety of human tumor cells. However, it is still elusive how the agent acts on tumors. Here we show that A549 and H1299 cells formed DNA double-strand breaks after 8-Cl-Ado exposure, accompanied by E2F1 upregulation at protein level. Overexpressed wild-type (E2F1-wt) colocalized with double-strand break marker gamma-H2AX and promoted G2/M arrest in 8-Cl-Ado-exposed A549 and H1299, while expressed S31A mutant of E2F1 (E2F1-mu) significantly reduced ability to accumulate at sites of DNA damage and G2/M arrest, suggesting that E2F1 is required for activating G2/M checkpoint pathway upon DNA damage. Transfection of either E2F1-wt or E2F1-mu plasmid promoted apoptosis in 8-Cl-Ado-exposed cells, indicating that 8-Cl-Ado may induce apoptosis in E2F1-dependent and E2F1-independent ways. These findings demonstrate that E2F1 plays a crucial role in 8-Cl-Ado-induced G2/M arrest but is dispensable for 8-Cl-Ado-induced apoptosis. These data also suggest that the mechanism of 8-Cl-Ado action is complicated.

语种: 英语
所属项目编号: 30200131 ; 30671062 ; 30770464 ; 30971449
项目资助者: National Natural Science Foundation of China ; Education Committee of Beijing
WOS记录号: WOS:000301793600004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53420
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
2.Hainan Med Coll, Dept Biochem, Haikou 571101, Peoples R China
3.Capital Univ Med Sci, Dept Biochem & Mol Biol, Beijing 100054, Peoples R China

Recommended Citation:
Duan, Hong-Ying,Cao, Ji-Xiang,Qi, Jun-Juan,et al. E2F1 enhances 8-Chloro-adenosine-induced G2/M arrest and apoptosis in A549 and H1299 lung cancer cells[J]. BIOCHEMISTRY-MOSCOW,2012,77(3):261-269.
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