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学科主题基础医学
E2F1 enhances 8-Chloro-adenosine-induced G2/M arrest and apoptosis in A549 and H1299 lung cancer cells
Duan, Hong-Ying1,2; Cao, Ji-Xiang1; Qi, Jun-Juan1; Wu, Guo-Sheng1; Li, Shu-Yan1; An, Guo-Shun1; Jia, Hong-Ti1,3; Cai, Wang-Wei2; Ni, Ju-Hua1
关键词E2f1 8-chloro-adenosine Dna Double-stranded Breaks G2/m Arrest Apoptosis
刊名BIOCHEMISTRY-MOSCOW
2012-03-01
DOI10.1134/S0006297912030042
77期:3页:261-269
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]NUCLEOTIDE EXCISION-REPAIR ; LEUKEMIA K562 CELLS ; DNA-DAMAGE ; TRANSCRIPTION FACTORS ; IN-VIVO ; INHIBITION ; FAMILY ; GROWTH ; DEATH ; GENE
英文摘要

The E2F1 transcription factor is a well known regulator of cell proliferation and apoptosis, but its role in response to DNA damage is less clear. 8-Chloro-adenosine (8-Cl-Ado), a nucleoside analog, can inhibit proliferation in a variety of human tumor cells. However, it is still elusive how the agent acts on tumors. Here we show that A549 and H1299 cells formed DNA double-strand breaks after 8-Cl-Ado exposure, accompanied by E2F1 upregulation at protein level. Overexpressed wild-type (E2F1-wt) colocalized with double-strand break marker gamma-H2AX and promoted G2/M arrest in 8-Cl-Ado-exposed A549 and H1299, while expressed S31A mutant of E2F1 (E2F1-mu) significantly reduced ability to accumulate at sites of DNA damage and G2/M arrest, suggesting that E2F1 is required for activating G2/M checkpoint pathway upon DNA damage. Transfection of either E2F1-wt or E2F1-mu plasmid promoted apoptosis in 8-Cl-Ado-exposed cells, indicating that 8-Cl-Ado may induce apoptosis in E2F1-dependent and E2F1-independent ways. These findings demonstrate that E2F1 plays a crucial role in 8-Cl-Ado-induced G2/M arrest but is dispensable for 8-Cl-Ado-induced apoptosis. These data also suggest that the mechanism of 8-Cl-Ado action is complicated.

语种英语
WOS记录号WOS:000301793600004
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53420
专题北京大学基础医学院
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
2.Hainan Med Coll, Dept Biochem, Haikou 571101, Peoples R China
3.Capital Univ Med Sci, Dept Biochem & Mol Biol, Beijing 100054, Peoples R China
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GB/T 7714
Duan, Hong-Ying,Cao, Ji-Xiang,Qi, Jun-Juan,et al. E2F1 enhances 8-Chloro-adenosine-induced G2/M arrest and apoptosis in A549 and H1299 lung cancer cells[J]. BIOCHEMISTRY-MOSCOW,2012,77(3):261-269.
APA Duan, Hong-Ying.,Cao, Ji-Xiang.,Qi, Jun-Juan.,Wu, Guo-Sheng.,Li, Shu-Yan.,...&Ni, Ju-Hua.(2012).E2F1 enhances 8-Chloro-adenosine-induced G2/M arrest and apoptosis in A549 and H1299 lung cancer cells.BIOCHEMISTRY-MOSCOW,77(3),261-269.
MLA Duan, Hong-Ying,et al."E2F1 enhances 8-Chloro-adenosine-induced G2/M arrest and apoptosis in A549 and H1299 lung cancer cells".BIOCHEMISTRY-MOSCOW 77.3(2012):261-269.
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