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学科主题: 基础医学
题名:
Characterization of a rabbit kidney prostaglandin F-2 alpha receptor exhibiting G(i)-restricted signaling that inhibits water absorption in the collecting duct
作者: Hebert, RL; Carmosino, M; Saito, O; Yang, GR; Jackson, CA; Qi, ZH; Breyer, RM; Natarajan, C; Hata, AN; Zhang, YH; Guan, YF; Breyer, MD
刊名: JOURNAL OF BIOLOGICAL CHEMISTRY
发表日期: 2005-10-14
DOI: 10.1074/jbc.M505852200
卷: 280, 期:41, 页:35028-35037
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: INTRACELLULAR CA2+ MOBILIZATION ; VASOPRESSIN-ELICITED WATER ; PROSTANOID FP RECEPTOR ; HAMSTER OVARY CELLS ; G-PROTEIN ; MESSENGER-RNA ; PGF(2-ALPHA) RECEPTOR ; MOLECULAR-CLONING ; SODIUM-TRANSPORT ; CORPORA-LUTEA
英文摘要:

PGF(2 alpha) is the most abundant prostaglandin detected in urine; however, its renal effects are poorly characterized. The present study cloned a PGF-prostanoid receptor (FP) from the rabbit kidney and determined the functional consequences of its activation. Nuclease protection assay showed that FP mRNA expression predominates in rabbit ovary and kidney. In situ hybridization revealed that renal FP expression predominates in the cortical collecting duct (CCD). Although FP receptor activation failed to increase intracellular Ca2+, it potently inhibited vasopressin-stimulated osmotic water permeability (L-p, 10(-7) cm/(atm.s)) in in vitro microperfused rabbit CCDs. Inhibition of Lp by the FP selective agonist latanoprost was additive to inhibition of vasopressin action by the EP selective agonist sulprostone. Inhibition of Lp by latanoprost was completely blocked by pertussis toxin, consistent with a G(i)-coupled mechanism. Heterologous transfection of the rabbit FPr into HEK293 cells also showed that latanoprost inhibited cAMP generation via a pertussis toxin-sensitive mechanism but did not increase cell Ca2+. These studies demonstrate a functional FP receptor on the basolateral membrane of rabbit CCDs. In contrast to the Ca2+ signal transduced by other FP receptors, this renal FP receptor signals via a PT-sensitive mechanism that is not coupled to cell Ca2+.

语种: 英语
WOS记录号: WOS:000232403900076
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53421
Appears in Collections:基础医学院_生理学与病理生理学系_期刊论文

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作者单位: 1.Vanderbilt Univ, Med Ctr, Div Nephrol, Nashville, TN 37232 USA
2.Univ Ottawa, Fac Med, Kidney Res Ctr, Dept Cellular, Ottawa, ON K1H 8M5, Canada
3.Univ Ottawa, Fac Med, Kidney Res Ctr, Dept Mol Med, Ottawa, ON K1H 8M5, Canada
4.Peking Univ, Hlth Sci Ctr, Dept Physiol, Beijing 100083, Peoples R China

Recommended Citation:
Hebert, RL,Carmosino, M,Saito, O,et al. Characterization of a rabbit kidney prostaglandin F-2 alpha receptor exhibiting G(i)-restricted signaling that inhibits water absorption in the collecting duct[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2005,280(41):35028-35037.
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