IR@PKUHSC  > 北京大学基础医学院  > 免疫学系
学科主题基础医学
Selective fluorescence of zymogen granules of pancreatic acinar cells stained with hematoxylin and eosin
Wu, Y; Li, B; Gao, XM
关键词acinar cells eosin Y fluorescence pancreas zymogen granules
刊名BIOTECHNIC & HISTOCHEMISTRY
2002-09-01
77期:5-6页:291-293
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Cell Biology
研究领域[WOS]Biotechnology & Applied Microbiology ; Cell Biology
关键词[WOS]MICROSCOPY ; SECTIONS
英文摘要

Following staining with hematoxylin and eosin Y, paraffin sections of mouse pancreas were examined by transmitted light, epifluorescence and confocal laser scanning microscopy. Light microscopy revealed that the nuclei of pancreatic acinar cells were located basally, while the apices of the cells appeared eosinophilic, although the secretory granules were difficult to visualize. Under violet-blue light excitation, the zymogen granules at the apices of the acinar cells showed strong yellowish fluorescence; the other part of the cytoplasm was only faintly fluorescent and the nuclei and the supporting tissues were nonfluorescent. Confocal laser scanning microscopy resulted in clear pictures of the zymogen granules and their distribution within the cell. The fluorescent emission of zymogen granules was certainly the result of eosin Y staining, because hematoxylin is not a fluorochrome and the zymogen granules are not autofluorescent. Staining with eosin Y alone, however, did not result in clear fluorescent images of zymogen granules or any other cellular structures. Our observation shows that the fluorescence emission of eosin Y allows easy and precise recognition of zymogen granules of pancreatic cells.

语种英语
WOS记录号WOS:000180013200004
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被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53427
专题北京大学基础医学院_免疫学系
作者单位Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China
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GB/T 7714
Wu, Y,Li, B,Gao, XM. Selective fluorescence of zymogen granules of pancreatic acinar cells stained with hematoxylin and eosin[J]. BIOTECHNIC & HISTOCHEMISTRY,2002,77(5-6):291-293.
APA Wu, Y,Li, B,&Gao, XM.(2002).Selective fluorescence of zymogen granules of pancreatic acinar cells stained with hematoxylin and eosin.BIOTECHNIC & HISTOCHEMISTRY,77(5-6),291-293.
MLA Wu, Y,et al."Selective fluorescence of zymogen granules of pancreatic acinar cells stained with hematoxylin and eosin".BIOTECHNIC & HISTOCHEMISTRY 77.5-6(2002):291-293.
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