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学科主题: 基础医学
题名:
Selective fluorescence of zymogen granules of pancreatic acinar cells stained with hematoxylin and eosin
作者: Wu, Y; Li, B; Gao, XM
关键词: acinar cells ; eosin Y ; fluorescence ; pancreas ; zymogen granules
刊名: BIOTECHNIC & HISTOCHEMISTRY
发表日期: 2002-09-01
卷: 77, 期:5-6, 页:291-293
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology ; Cell Biology
研究领域[WOS]: Biotechnology & Applied Microbiology ; Cell Biology
关键词[WOS]: MICROSCOPY ; SECTIONS
英文摘要:

Following staining with hematoxylin and eosin Y, paraffin sections of mouse pancreas were examined by transmitted light, epifluorescence and confocal laser scanning microscopy. Light microscopy revealed that the nuclei of pancreatic acinar cells were located basally, while the apices of the cells appeared eosinophilic, although the secretory granules were difficult to visualize. Under violet-blue light excitation, the zymogen granules at the apices of the acinar cells showed strong yellowish fluorescence; the other part of the cytoplasm was only faintly fluorescent and the nuclei and the supporting tissues were nonfluorescent. Confocal laser scanning microscopy resulted in clear pictures of the zymogen granules and their distribution within the cell. The fluorescent emission of zymogen granules was certainly the result of eosin Y staining, because hematoxylin is not a fluorochrome and the zymogen granules are not autofluorescent. Staining with eosin Y alone, however, did not result in clear fluorescent images of zymogen granules or any other cellular structures. Our observation shows that the fluorescence emission of eosin Y allows easy and precise recognition of zymogen granules of pancreatic cells.

语种: 英语
WOS记录号: WOS:000180013200004
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53427
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China

Recommended Citation:
Wu, Y,Li, B,Gao, XM. Selective fluorescence of zymogen granules of pancreatic acinar cells stained with hematoxylin and eosin[J]. BIOTECHNIC & HISTOCHEMISTRY,2002,77(5-6):291-293.
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