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学科主题基础医学
Shu-Gan-Liang-Xue Decoction, a Chinese herbal formula, down-regulates the expression of steroid sulfatase genes in human breast carcinoma MCF-7 cells
Zhang, Yi2,3; Li, Ping-Ping1
关键词Breast Cancer Steroid Sulfatase Estrogen Biosynthesis Shu-gan-liang-xue Decoction In Vitro
刊名JOURNAL OF ETHNOPHARMACOLOGY
2010-02-17
DOI10.1016/j.jep.2009.12.014
127期:3页:620-624
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
研究领域[WOS]Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
关键词[WOS]CANCER PATIENTS ; ESTROGEN SULFOTRANSFERASE ; AROMATASE ; SHIKONIN ; INHIBITION ; THERAPY ; GROWTH ; PROLIFERATION ; ENDOMETRIAL ; PATHWAY
英文摘要

Ethnopharmacological relevance: Shu-Gan-Liang-Xue Decoction (SGLXD), a traditional Chinese herbal formula, has been used to ameliorate hot flushes symptom in breast cancer patients for decades.

Aim of the study: Steroid sulfatase (STS) has a crucial role in regulating the estrogen biosynthesis within breast tumors. We aimed to investigate whether SGLXD could modulate STS expression in human breast carcinoma MCF-7 cells.

Materials and methods: By semi-quantitative/quantitative reverse transcription-PCR, we investigated the transcript level of STS in MCF-7 cells treated with various concentrations of SGLXD. By using transient cotransfection with estrogen dependent plasmid pERE-TK-Luc and internal control plasmid pRL-TK in MCF-7 cells and dual luciferase reporter (DLR) based bioluminescent measurements, we evaluated the enzymatic activity of STS after SGLXD treatment.

Results: By RT-PCR and real time PCR, the mRNA level of STS was decreased by SGLXD treatment, in the dose-dependent manner, compared to negative control (p < 0.01). By DLR assay, different concentrations of SGLXD significantly inhibited the enzymatic activity of STS in MCF-7 cells dose-dependently (p < 0.05).

Conclusions: SGLXD could act as a selective estrogen enzyme modulator by down-regulating the STS expression in MCF-7 cells. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000275628600007
项目编号2004-IV15
资助机构Key Program Foundation of Beijing Administration of Traditional Chinese Medicines, China
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53428
专题北京大学基础医学院
北京大学临床肿瘤学院_中西医结合科暨老年肿瘤科
作者单位1.Peking Univ, Key Lab Carcinogenesis & Translat Res, Minist Educ,Sch Oncol,Beijing Canc Hosp & Inst, Dept Integrated Tradit Chinese & Western Med, Beijing 100142, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Integrated Tradit Chinese & Western Med, Beijing 100191, Peoples R China
3.Beijing Univ Chinese Med, Dept Anat, Sch Preclin Med, Beijing 100029, Peoples R China
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GB/T 7714
Zhang, Yi,Li, Ping-Ping. Shu-Gan-Liang-Xue Decoction, a Chinese herbal formula, down-regulates the expression of steroid sulfatase genes in human breast carcinoma MCF-7 cells[J]. JOURNAL OF ETHNOPHARMACOLOGY,2010,127(3):620-624.
APA Zhang, Yi,&Li, Ping-Ping.(2010).Shu-Gan-Liang-Xue Decoction, a Chinese herbal formula, down-regulates the expression of steroid sulfatase genes in human breast carcinoma MCF-7 cells.JOURNAL OF ETHNOPHARMACOLOGY,127(3),620-624.
MLA Zhang, Yi,et al."Shu-Gan-Liang-Xue Decoction, a Chinese herbal formula, down-regulates the expression of steroid sulfatase genes in human breast carcinoma MCF-7 cells".JOURNAL OF ETHNOPHARMACOLOGY 127.3(2010):620-624.
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