北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 期刊论文
学科主题: 基础医学
题名:
Shu-Gan-Liang-Xue Decoction, a Chinese herbal formula, down-regulates the expression of steroid sulfatase genes in human breast carcinoma MCF-7 cells
作者: Zhang, Yi2,3; Li, Ping-Ping1
关键词: Breast cancer ; Steroid sulfatase ; Estrogen biosynthesis ; Shu-Gan-Liang-Xue Decoction ; In vitro
刊名: JOURNAL OF ETHNOPHARMACOLOGY
发表日期: 2010-02-17
DOI: 10.1016/j.jep.2009.12.014
卷: 127, 期:3, 页:620-624
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
研究领域[WOS]: Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
关键词[WOS]: CANCER PATIENTS ; ESTROGEN SULFOTRANSFERASE ; AROMATASE ; SHIKONIN ; INHIBITION ; THERAPY ; GROWTH ; PROLIFERATION ; ENDOMETRIAL ; PATHWAY
英文摘要:

Ethnopharmacological relevance: Shu-Gan-Liang-Xue Decoction (SGLXD), a traditional Chinese herbal formula, has been used to ameliorate hot flushes symptom in breast cancer patients for decades.

Aim of the study: Steroid sulfatase (STS) has a crucial role in regulating the estrogen biosynthesis within breast tumors. We aimed to investigate whether SGLXD could modulate STS expression in human breast carcinoma MCF-7 cells.

Materials and methods: By semi-quantitative/quantitative reverse transcription-PCR, we investigated the transcript level of STS in MCF-7 cells treated with various concentrations of SGLXD. By using transient cotransfection with estrogen dependent plasmid pERE-TK-Luc and internal control plasmid pRL-TK in MCF-7 cells and dual luciferase reporter (DLR) based bioluminescent measurements, we evaluated the enzymatic activity of STS after SGLXD treatment.

Results: By RT-PCR and real time PCR, the mRNA level of STS was decreased by SGLXD treatment, in the dose-dependent manner, compared to negative control (p < 0.01). By DLR assay, different concentrations of SGLXD significantly inhibited the enzymatic activity of STS in MCF-7 cells dose-dependently (p < 0.05).

Conclusions: SGLXD could act as a selective estrogen enzyme modulator by down-regulating the STS expression in MCF-7 cells. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
所属项目编号: 2004-IV15
项目资助者: Key Program Foundation of Beijing Administration of Traditional Chinese Medicines, China
WOS记录号: WOS:000275628600007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53428
Appears in Collections:基础医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Key Lab Carcinogenesis & Translat Res, Minist Educ,Sch Oncol,Beijing Canc Hosp & Inst, Dept Integrated Tradit Chinese & Western Med, Beijing 100142, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Integrated Tradit Chinese & Western Med, Beijing 100191, Peoples R China
3.Beijing Univ Chinese Med, Dept Anat, Sch Preclin Med, Beijing 100029, Peoples R China

Recommended Citation:
Zhang, Yi,Li, Ping-Ping. Shu-Gan-Liang-Xue Decoction, a Chinese herbal formula, down-regulates the expression of steroid sulfatase genes in human breast carcinoma MCF-7 cells[J]. JOURNAL OF ETHNOPHARMACOLOGY,2010,127(3):620-624.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, Yi]'s Articles
[Li, Ping-Ping]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, Yi]‘s Articles
[Li, Ping-Ping]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  北京大学医学部 - Feedback
Powered by CSpace