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The protective effect of CD8(+)CD28(-) T suppressor cells on the acute rejection responses in rat liver transplantation
Liu, Y.; Chen, N.; Chen, G.; You, P.
刊名TRANSPLANTATION PROCEEDINGS
2007-12-01
DOI10.1016/j.transproceed.2007.06.089
39期:10页:3396-3403
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology ; Surgery ; Transplantation
研究领域[WOS]Immunology ; Surgery ; Transplantation
关键词[WOS]MIXED LYMPHOCYTE-REACTION ; SPONTANEOUS ACCEPTANCE ; OPERATIONAL TOLERANCE ; BLOOD-TRANSFUSION ; REGULATORY CELLS ; ALLOGRAFTS ; MAINTENANCE ; INDUCTION
英文摘要

Regulatory T cells (Tr) or T-suppressor cells (Ts), which include CD4(+)CD25(+) T cells and CD8(+)CD28(-) T cells respectively, have been shown to be essential for the induction and maintenance of immune tolerance. We have investigated the effect of CD8(+)CD28(-) Ts and CD4(+)CD25(+) Tr on acute rejection responses in rat liver transplantation (OLT).

Methods. CD8(+)CD28(-) Ts/CD4(+)CD25(+) Tr were obtained from inbred and naive rats that show spontaneous tolerance to OLT. Adoptive transfers were performed in acute rejection models of various strain combinations with survival times observed to evaluate suppressive effects. Donor-specific blood transfusion (DST) was used to induce CD8(+)CD28(-) Ts in naive rats, which were assayed in vitro using carboxyfluorescein diacetate succinimidyl easter-labeled one-way mixed lymphocyte reactions. Secondary adoptive transfers of DST-induced CD8(+)CD28(-) Ts were also performed in an acute OLT rejection model.

Results. CDS(+)CD28(-) Ts from tolerant OLT model rats possessed immunosuppressive activity in allogeneic recipients; adoptive transfers of these cells alleviated the acute rejection responses. However, CD4(+)CD25(+) Tr derived from tolerant or naive rats failed to do so. In vitro DST-induced CD8(+)CD28(-) Ts inhibited alloantigen T-cell responses in naive syngeneic rats in an antigen-specific manner. Secondary adoptive transfer of DST-induced CD8(+)CD28(-)Ts further reduced acute rejection but not chronic rejection responses.

Conclusions. CD8(+)CD28(-) Ts cells protected allogeneic recipients from acute rejection in rat OLT. Furthermore, this activity was not present in CD4(+)CD25(+)Tr. DST was observed to be an effective method to generate functional CD8(+)CD28(-)Ts in naive rats.

语种英语
WOS记录号WOS:000252037900095
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53447
专题北京大学第二临床医学院_消化科
作者单位Peking Univ, Peoples Hosp, Dept Gastroenterol, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Liu, Y.,Chen, N.,Chen, G.,et al. The protective effect of CD8(+)CD28(-) T suppressor cells on the acute rejection responses in rat liver transplantation[J]. TRANSPLANTATION PROCEEDINGS,2007,39(10):3396-3403.
APA Liu, Y.,Chen, N.,Chen, G.,&You, P..(2007).The protective effect of CD8(+)CD28(-) T suppressor cells on the acute rejection responses in rat liver transplantation.TRANSPLANTATION PROCEEDINGS,39(10),3396-3403.
MLA Liu, Y.,et al."The protective effect of CD8(+)CD28(-) T suppressor cells on the acute rejection responses in rat liver transplantation".TRANSPLANTATION PROCEEDINGS 39.10(2007):3396-3403.
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