北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 消化内科  > 期刊论文
学科主题: 临床医学
题名:
The protective effect of CD8(+)CD28(-) T suppressor cells on the acute rejection responses in rat liver transplantation
作者: Liu, Y.; Chen, N.; Chen, G.; You, P.
刊名: TRANSPLANTATION PROCEEDINGS
发表日期: 2007-12-01
DOI: 10.1016/j.transproceed.2007.06.089
卷: 39, 期:10, 页:3396-3403
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology ; Surgery ; Transplantation
研究领域[WOS]: Immunology ; Surgery ; Transplantation
关键词[WOS]: MIXED LYMPHOCYTE-REACTION ; SPONTANEOUS ACCEPTANCE ; OPERATIONAL TOLERANCE ; BLOOD-TRANSFUSION ; REGULATORY CELLS ; ALLOGRAFTS ; MAINTENANCE ; INDUCTION
英文摘要:

Regulatory T cells (Tr) or T-suppressor cells (Ts), which include CD4(+)CD25(+) T cells and CD8(+)CD28(-) T cells respectively, have been shown to be essential for the induction and maintenance of immune tolerance. We have investigated the effect of CD8(+)CD28(-) Ts and CD4(+)CD25(+) Tr on acute rejection responses in rat liver transplantation (OLT).

Methods. CD8(+)CD28(-) Ts/CD4(+)CD25(+) Tr were obtained from inbred and naive rats that show spontaneous tolerance to OLT. Adoptive transfers were performed in acute rejection models of various strain combinations with survival times observed to evaluate suppressive effects. Donor-specific blood transfusion (DST) was used to induce CD8(+)CD28(-) Ts in naive rats, which were assayed in vitro using carboxyfluorescein diacetate succinimidyl easter-labeled one-way mixed lymphocyte reactions. Secondary adoptive transfers of DST-induced CD8(+)CD28(-) Ts were also performed in an acute OLT rejection model.

Results. CDS(+)CD28(-) Ts from tolerant OLT model rats possessed immunosuppressive activity in allogeneic recipients; adoptive transfers of these cells alleviated the acute rejection responses. However, CD4(+)CD25(+) Tr derived from tolerant or naive rats failed to do so. In vitro DST-induced CD8(+)CD28(-) Ts inhibited alloantigen T-cell responses in naive syngeneic rats in an antigen-specific manner. Secondary adoptive transfer of DST-induced CD8(+)CD28(-)Ts further reduced acute rejection but not chronic rejection responses.

Conclusions. CD8(+)CD28(-) Ts cells protected allogeneic recipients from acute rejection in rat OLT. Furthermore, this activity was not present in CD4(+)CD25(+)Tr. DST was observed to be an effective method to generate functional CD8(+)CD28(-)Ts in naive rats.

语种: 英语
WOS记录号: WOS:000252037900095
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53447
Appears in Collections:北京大学第二临床医学院_消化内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Peoples Hosp, Dept Gastroenterol, Beijing 100044, Peoples R China

Recommended Citation:
Liu, Y.,Chen, N.,Chen, G.,et al. The protective effect of CD8(+)CD28(-) T suppressor cells on the acute rejection responses in rat liver transplantation[J]. TRANSPLANTATION PROCEEDINGS,2007,39(10):3396-3403.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, Y.]'s Articles
[Chen, N.]'s Articles
[Chen, G.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, Y.]‘s Articles
[Chen, N.]‘s Articles
[Chen, G.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace