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学科主题: 临床医学
题名:
Stromal cell-derived factor 1 alpha stimulates human endometrial carcinoma cell growth through the activation of both extracellular signal-regulated kinase 1/2 and Akt
作者: Zhao, Dan; Li, Xiao-ping; Gao, Min; Zhao, Chao; Wang, Jian-liu; Wei, Li-hui
关键词: stromal cell-derived factor ; human endometrial carcinoma cell
刊名: GYNECOLOGIC ONCOLOGY
发表日期: 2006-12-01
DOI: 10.1016/j.ygyno.2006.05.045
卷: 103, 期:3, 页:932-937
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Obstetrics & Gynecology
研究领域[WOS]: Oncology ; Obstetrics & Gynecology
关键词[WOS]: EXPRESSION ; CANCER ; PTEN ; RECEPTORS
英文摘要:

Objective. The aim of study was to investigate the proliferative effects of stromal cell-derived factor-la (SDF-1 alpha) on endometrial carcinomas cell lines with different estrogen receptors (ER) and PTEN protein profiles.

Methods. MTT assays was used to detect the proliferation of HEC-1A and lshikawa cells, and Western blotted analysis was used to detect activation of Akt and ERK1/2 in both cell lines after exposure to various concentrations of SDF-1 alpha, MAPK-specific inhibitor PD98059 or PI3K-specific inhibitor LY294002.

Results. Low concentrations of SDF-1 alpha (<= 50 ng/ml) significantly promoted proliferation of Ishikawa cells but not of HEC-1A, while high concentrations of SDF-1 alpha (> 50 ng/ml) induced proliferation in both cell lines. ERK1/2 was significantly activated for more than 2 It by SDF-1 alpha at 20 ng/ml in HEC-1A cells, but not in Ishikawa cells. In contrast, Akt was significantly activated for over 2 It in Ishikawa cells but remained unchanged in HEC-1A cells. High concentrations of SDF-1 alpha activated Akt and ERK 1/2 pathways in both cell lines in a dose-dependent manner, which was primarily inhibited by LY294002 for pAkt and by PD98059 for pERK 1/2.

Conclusions. SDF-1 alpha could stimulate the cell proliferation of endometrial carcinoma with different expression status of ER and PTEN in vitro, likely through the activation of both Akt and ERK1/2 signaling pathways. (c) 2006 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000242809500026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53449
Appears in Collections:北京大学第二临床医学院_妇科_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Dept Gynecol, Beijing 100044, Peoples R China

Recommended Citation:
Zhao, Dan,Li, Xiao-ping,Gao, Min,et al. Stromal cell-derived factor 1 alpha stimulates human endometrial carcinoma cell growth through the activation of both extracellular signal-regulated kinase 1/2 and Akt[J]. GYNECOLOGIC ONCOLOGY,2006,103(3):932-937.
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