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学科主题: 基础医学
题名:
Mechanism of interleukin-1 beta-induced calcitonin gene-related peptide production from dorsal root ganglion neurons of neonatal rats
作者: Hou, LF; Li, WJ; Wang, X
关键词: interleukin-1 beta ; calcitonin gene-related peptide ; dorsal root ganglion
刊名: JOURNAL OF NEUROSCIENCE RESEARCH
发表日期: 2003-07-15
DOI: 10.1002/jnr.10651
卷: 73, 期:2, 页:188-197
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: PRIMARY SENSORY NEURONS ; NECROSIS-FACTOR-ALPHA ; CGRP MESSENGER-RNAS ; SPINAL-CORD ; SUBSTANCE-P ; BETA-CGRP ; DIFFERENTIAL EXPRESSION ; PERIPHERAL INFLAMMATION ; NERVOUS-SYSTEM ; NEUROPEPTIDE
英文摘要:

Calcitonin gene-related peptide (CGRP) is synthesized in dorsal root ganglion (DRG) neurons and released from primary afferent neurons to mediate hemodynamic effects and neurogenic inflammation. The effect of the proinflammatory cytokine interleukin-1 (IL-1)-beta on CGRP release from these sensory neurons was investigated. The results showed that IL-1beta (1 ng/ml) could directly induce CGRP release following prolonged incubation (24 hr) with these neurons. Treatment with IL-1beta (0.1-1.0 ng/ml) significantly increased CGRP release in a concentration-dependent manner. In addition, pretreatment of DRG cells with actinomycin D at 1 muM or cyclohexamide at 10 muM for 30 min inhibited 1 ng/ml IL-1beta-induced CGRP release in DRG neurons of neonatal rats. The inhibitors of PKC, JNK MAPK and NF-kappaB, but not p38 or ERK1/2 MAPK, blocked IL-1beta-induced CGRP release. RNase protection assay showed that IL-1beta could cause alpha-CGRP mRNA increase in a time- and concentration-dependent manner, although the level of P-CGRP mRNA was not affected. These results indicate that IL-1beta may activate PKC, which in turn initiates JNK MAPK and activates NF-kappaB and finally induces alpha-CGRP gene expression and release from these sensory neurons. (C) 2003 Wiley-Liss, Inc.

语种: 英语
WOS记录号: WOS:000183930200005
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53465
Appears in Collections:基础医学院_生理学与病理生理学系_期刊论文

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作者单位: 1.Peking Univ, Dept Physiol, Hlth Sci Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100871, Peoples R China
3.Peking Univ, Reference Lab Educ Minist Mol Cardiol, Hlth Sci Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Hou, LF,Li, WJ,Wang, X. Mechanism of interleukin-1 beta-induced calcitonin gene-related peptide production from dorsal root ganglion neurons of neonatal rats[J]. JOURNAL OF NEUROSCIENCE RESEARCH,2003,73(2):188-197.
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