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学科主题: 公共卫生
题名:
Effects of repeated Cr(VI) intratracheal instillation on club (Clara) cells and activation of nuclear factor-kappa B pathway via oxidative stress
作者: Zhao, Lin; Song, Yanshuang; Pu, Ji; Guo, Jian; Wang, Yun; Chen, Zhangjian; Chen, Tian; Gu, Yongen; Jia, Guang
关键词: Hexavalent chromium ; Club (Clara) cell ; Biomarker ; NF-kappa B ; Oxidative stress ; Animal experiment
刊名: TOXICOLOGY LETTERS
发表日期: 2014-11-18
DOI: 10.1016/j.toxlet.2014.09.011
卷: 231, 期:1, 页:72-81
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
关键词[WOS]: SECRETORY PROTEIN ; CHROMATE WORKERS ; DNA-DAMAGE ; CHROMIUM ACCUMULATION ; TRANSCRIPTION FACTOR ; LUNG ; ZINC ; APOPTOSIS ; EXPRESSION ; EPITHELIUM
英文摘要:

Hexavalent chromium [Cr(VI)] exposure is known to induce respiratory inflammation and contribute to lung cancer development, but little is known about its target cell type in lung. In the current study, we investigated the effects of repeated Cr(VI) intratracheal instillation on club (Clara) cells and club (Clara) cell secretory protein (CC16) in rats and explored whether the nuclear factor-kappa B (NF-kappa B) related pathway was involved. We also studied the role of orally delivered Zn against Cr-induced adverse health effects. For four weeks, sixty Sprague-Dawley male rats received weekly intratracheal instillation of potassium dichromate (K2Cr2O7) at 0, 0.063 and 0.630 mg Cr/kg with or without daily intragastric administration of zinc sulfate (ZnSO4) at 10 mg Zn/kg. Results showed that exposure to Cr(VI) significantly increased the organ coefficient of lung (organ weight as a percentage of body weight), albumin and total protein level in bronchoalveolar lavage fluid (BALF), indicating lung injury and compromised bronchoalveolar/blood barrier (BA/BB) integrity. With increasing Cr(VI) dose, the secretion of CC16 decreased in a dose-dependent manner, suggesting that CC16 can serve as a peripheral biomarker for club cell damage during early lung injury induced by Cr(VI). Increased expression of NF-kappa B were observed in club cells in both Cr-exposed groups, indicating upregulation of NF-kappa B, which can be induced by reactive oxygen species (ROS) generated by club cells during Cr reduction with repetitive Cr(VI) exposure. Cr-induced DNA damage was also observed, as significant increase of 8-OHdG was found with Cr exposure at 0.630 mg/kg week. Oral Zn supplementation did not alleviate changes in serum CC16 level under Cr(VI) exposure, indicating its failure in protecting against Cr(VI)-induced club cell damage. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
所属项目编号: 81072281 ; 81273043 ; 20120001110103
项目资助者: National Natural Science Foundation of China ; Doctoral Fund of Ministry of Education of China
WOS记录号: WOS:000344696100009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53466
Appears in Collections:北京大学公共卫生学院_期刊论文

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作者单位: Peking Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth Sci, Beijing 100191, Peoples R China

Recommended Citation:
Zhao, Lin,Song, Yanshuang,Pu, Ji,et al. Effects of repeated Cr(VI) intratracheal instillation on club (Clara) cells and activation of nuclear factor-kappa B pathway via oxidative stress[J]. TOXICOLOGY LETTERS,2014,231(1):72-81.
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