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Non-virus-mediated transfer of siRNAs against Runx2 and Smad4 inhibit heterotopic ossification in rats
Xue, T.1; Mao, Z.2; Lin, L.1; Hou, Y.1; Wei, X.3; Fu, X.1; Zhang, J.1; Yu, C.1
关键词Sirna Runx2 Smad4 Non-virus Heterotopic Ossification
刊名GENE THERAPY
2010-03-01
DOI10.1038/gt.2009.154
17期:3页:370-379
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity ; Research & Experimental Medicine
关键词[WOS]TOTAL HIP-ARTHROPLASTY ; NONSTEROIDAL ANTIINFLAMMATORY DRUGS ; BONE MORPHOGENETIC PROTEIN-2 ; TRAUMATIC BRAIN-INJURY ; OSTEOBLAST DIFFERENTIATION ; CLEIDOCRANIAL DYSPLASIA ; ECTOPIC OSSIFICATION ; ENHANCED OSTEOGENESIS ; GENE-EXPRESSION ; RISK-FACTORS
英文摘要

Heterotopic ossification of muscles, tendons and ligaments are a common problem affecting patient with trauma or received elective surgery. But the existing preventive or therapeutic methods all have disadvantages. Runt-related protein 2 (Runx2) and Smad4 are two regulators that have important roles in the differentiation of osteoblast. In this study, we attempted to examine the effect of Runx2 and Smad4 on the development of heterotopic ossification in vitro. We constructed non-virus-containing small interference RNAs (siRNAs) against Runx2 and Smad4 and tested it with reverse transcriptase-PCR and western blot. We then analyzed the independent effect of Runx2- and Smad4-specific siRNAs and their cooperative effect on the formation of heterotopic ossification induced by trauma in rats. The effects were measured with computed tomography scanning, hematoxylin and eosin staining and immunohistochemistry. We found that the Runx2 and Smad4-specific siRNAs inhibited the expression of Runx2 and Smad4 at the level of messenger RNA and protein. Runx2 and Smad4 independently inhibited the formation of heterotopic ossification. Moreover, their co-expression significantly enhanced the inhibition of heterotopic ossification compared with the independent effect. We suggest that gene therapy to inhibit Runx2 and Smad4 by RNAi could be a powerful approach to prevent or treat heterotopic ossification. Gene Therapy (2010) 17, 370-379; doi:10.1038/gt.2009.154; published online 26 November 2009

语种英语
WOS记录号WOS:000275392600008
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53477
专题北京大学第三临床医学院_运动医学研究所
北京大学第一临床医学院_急诊科
作者单位1.Tianjin Hosp, Dept Orthoped Surg, Tianjin, Peoples R China
2.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100191, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100871, Peoples R China
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GB/T 7714
Xue, T.,Mao, Z.,Lin, L.,et al. Non-virus-mediated transfer of siRNAs against Runx2 and Smad4 inhibit heterotopic ossification in rats[J]. GENE THERAPY,2010,17(3):370-379.
APA Xue, T..,Mao, Z..,Lin, L..,Hou, Y..,Wei, X..,...&Yu, C..(2010).Non-virus-mediated transfer of siRNAs against Runx2 and Smad4 inhibit heterotopic ossification in rats.GENE THERAPY,17(3),370-379.
MLA Xue, T.,et al."Non-virus-mediated transfer of siRNAs against Runx2 and Smad4 inhibit heterotopic ossification in rats".GENE THERAPY 17.3(2010):370-379.
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