|Tetrandrine Suppresses Lipopolysaccharide-Induced Microglial Activation by Inhibiting NF-kappa B and ERK Signaling Pathways in BV2 Cells|
|Dang, Yalong1,2,3; Xu, Yongsheng2,3; Wu, Wentao2; Li, Weiyi2,3; Sun, Yanran3; Yang, Jing1; Zhu, Yu1; Zhang, Chun2,3|
|WOS标题词||Science & Technology|
|研究领域[WOS]||Science & Technology - Other Topics|
|关键词[WOS]||RETINAL GANGLION-CELLS ; TUMOR-NECROSIS-FACTOR ; OPTIC-NERVE HEAD ; OPEN-ANGLE GLAUCOMA ; FOCAL CEREBRAL-ISCHEMIA ; FACTOR-ALPHA ; MOUSE MODEL ; GENE-EXPRESSION ; NEUROPROTECTION ; INFLAMMATION|
Background and Objective: Tetrandrine (TET) is a bisbenzylisoquinoline alkaloid extracted from Stephania tetrandra Moore. Recent studies have suggested that TET can reduce the inflammatory response in microglia, but the mechanisms remain unclear. The aim of this study is to investigate whether TET can inhibit lipopolysaccharide (LPS)-induced microglial activation and clarify its possible mechanisms.
Study Design/Materials and Methods: Cell viability assays and cell apoptosis assays were used to determine the working concentrations of TET. Then, BV2 cells were seeded and pretreated with TET for 2 h. LPS was then added and incubated for an additional 24 hours. qRT-PCR and ELISA were used to measure the mRNA or protein levels of IL1 beta and TNF alpha. Western blotting was utilized to quantify the expression of CD11b and cell signaling proteins.
Results: TET at optimal concentrations (0.1 mu M, 0.5 mu M or 1 mu M) did not affect the cell viability. After TET pretreatment, the levels of IL1 beta and TNF alpha (both in transcription and translation) were significantly inhibited in a dose-dependent manner. Further studies indicated that phospho-p65, phospho-IKK, and phospho-ERK 1/2 expression were also suppressed by TET.
Conclusions: Our results indicate that TET can effectively suppress microglial activation and inhibit the production of IL1 beta and TNF alpha by regulating the NF-kappa B and ERK signaling pathways. Together with our previous studies, we suggest that TET would be a promising candidate to effectively suppress overactivated microglia and alleviate neurodegeneration in glaucoma.
|作者单位||1.Zhengzhou Univ, Affiliated Hosp 1, Dept Ophthalmol, Zhengzhou 450052, Henan Province, Peoples R China|
2.Peking Univ, Hosp 3, Clin Stem Cell Res Ctr, Beijing 100871, Peoples R China
3.Peking Univ, Hosp 3, Dept Ophthalmol, Beijing 100871, Peoples R China
|Dang, Yalong,Xu, Yongsheng,Wu, Wentao,et al. Tetrandrine Suppresses Lipopolysaccharide-Induced Microglial Activation by Inhibiting NF-kappa B and ERK Signaling Pathways in BV2 Cells[J]. PLOS ONE,2014,9(8).|
|APA||Dang, Yalong.,Xu, Yongsheng.,Wu, Wentao.,Li, Weiyi.,Sun, Yanran.,...&Zhang, Chun.(2014).Tetrandrine Suppresses Lipopolysaccharide-Induced Microglial Activation by Inhibiting NF-kappa B and ERK Signaling Pathways in BV2 Cells.PLOS ONE,9(8).|
|MLA||Dang, Yalong,et al."Tetrandrine Suppresses Lipopolysaccharide-Induced Microglial Activation by Inhibiting NF-kappa B and ERK Signaling Pathways in BV2 Cells".PLOS ONE 9.8(2014).|