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学科主题: 临床医学
题名:
Tetrandrine Suppresses Lipopolysaccharide-Induced Microglial Activation by Inhibiting NF-kappa B and ERK Signaling Pathways in BV2 Cells
作者: Dang, Yalong1,2,3; Xu, Yongsheng2,3; Wu, Wentao2; Li, Weiyi2,3; Sun, Yanran3; Yang, Jing1; Zhu, Yu1; Zhang, Chun2,3
刊名: PLOS ONE
发表日期: 2014-08-12
DOI: 10.1371/journal.pone.0102522
卷: 9, 期:8
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: RETINAL GANGLION-CELLS ; TUMOR-NECROSIS-FACTOR ; OPTIC-NERVE HEAD ; OPEN-ANGLE GLAUCOMA ; FOCAL CEREBRAL-ISCHEMIA ; FACTOR-ALPHA ; MOUSE MODEL ; GENE-EXPRESSION ; NEUROPROTECTION ; INFLAMMATION
英文摘要:

Background and Objective: Tetrandrine (TET) is a bisbenzylisoquinoline alkaloid extracted from Stephania tetrandra Moore. Recent studies have suggested that TET can reduce the inflammatory response in microglia, but the mechanisms remain unclear. The aim of this study is to investigate whether TET can inhibit lipopolysaccharide (LPS)-induced microglial activation and clarify its possible mechanisms.

Study Design/Materials and Methods: Cell viability assays and cell apoptosis assays were used to determine the working concentrations of TET. Then, BV2 cells were seeded and pretreated with TET for 2 h. LPS was then added and incubated for an additional 24 hours. qRT-PCR and ELISA were used to measure the mRNA or protein levels of IL1 beta and TNF alpha. Western blotting was utilized to quantify the expression of CD11b and cell signaling proteins.

Results: TET at optimal concentrations (0.1 mu M, 0.5 mu M or 1 mu M) did not affect the cell viability. After TET pretreatment, the levels of IL1 beta and TNF alpha (both in transcription and translation) were significantly inhibited in a dose-dependent manner. Further studies indicated that phospho-p65, phospho-IKK, and phospho-ERK 1/2 expression were also suppressed by TET.

Conclusions: Our results indicate that TET can effectively suppress microglial activation and inhibit the production of IL1 beta and TNF alpha by regulating the NF-kappa B and ERK signaling pathways. Together with our previous studies, we suggest that TET would be a promising candidate to effectively suppress overactivated microglia and alleviate neurodegeneration in glaucoma.

语种: 英语
所属项目编号: 81371017 ; 13A320427 ; 2013GH11
项目资助者: National Natural Science Foundation of China ; Key Project of Science Research of Henan Province Education Committee ; Henan Province
WOS记录号: WOS:000341230400005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53482
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Zhengzhou Univ, Affiliated Hosp 1, Dept Ophthalmol, Zhengzhou 450052, Henan Province, Peoples R China
2.Peking Univ, Hosp 3, Clin Stem Cell Res Ctr, Beijing 100871, Peoples R China
3.Peking Univ, Hosp 3, Dept Ophthalmol, Beijing 100871, Peoples R China

Recommended Citation:
Dang, Yalong,Xu, Yongsheng,Wu, Wentao,et al. Tetrandrine Suppresses Lipopolysaccharide-Induced Microglial Activation by Inhibiting NF-kappa B and ERK Signaling Pathways in BV2 Cells[J]. PLOS ONE,2014,9(8).
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