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学科主题: 基础医学
题名:
beta-Catenin/LEF1 activated enamelin expression in ameloblast-like cells
作者: Tian, Hua2,3; Lv, Ping2,3; Ma, Kangtao1; Zhou, Chunyan1; Gao, Xuejun2,3
关键词: Enamelin ; Wnt/beta-catenin ; LEF1 ; Amelogenesis imperfecta ; Tooth development
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2010-07-30
DOI: 10.1016/j.bbrc.2010.06.111
卷: 398, 期:3, 页:519-524
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: DOMINANT AMELOGENESIS IMPERFECTA ; TISSUE-SPECIFIC EXPRESSION ; TOOTH MORPHOGENESIS ; MOUSE ; CATENIN ; MICE ; ESTABLISHMENT ; TRANSCRIPTION ; ELEMENTS ; LEF1
英文摘要:

Enamelin is an ameloblast-specific matrix protein believed to play essential roles in enamel formation. However, mechanisms of enamelin transcription regulation are not clear. beta-Catenin/LEF1 is a key transcriptional complex involved in tooth development. In this study, the role of beta-catenin/LEF1 in enamelin expression was investigated. The 5′-flanking region of the mouse enamelin gene was analyzed and cloned. Co-transfection analysis and mutation assays revealed that two conserved LEF1 responsive elements located at -1002 and -597 bp upstream of the enamelin translation initiation site could augment transcriptional activity of the enamelin. The interaction between the enamelin elements and beta-catenin/LEF1 was further confirmed by electrophoresis mobility shift assays and chromatin immunoprecipitation assays. In addition, LiCl treatment induced nuclear translocation of beta-catenin and elevated endogenous enamelin expression in mouse ameloblast-like cells. The results suggested that Wnt/beta-catenin signaling could function in enamelin gene expression by direct interaction through two conserved LEF1 responsive elements on the enamelin gene in ameloblast-like cells. (C) 2010 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: NSFC 30572033
项目资助者: Natural Science Foundation of China
WOS记录号: WOS:000280867400032
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53500
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
2.Peking Univ, Sch Stomatol, Dept Cariol & Endodontol, Beijing 100191, Peoples R China
3.Peking Univ, Hosp Stomatol, Dept Cariol & Endodontol, Beijing 100191, Peoples R China

Recommended Citation:
Tian, Hua,Lv, Ping,Ma, Kangtao,et al. beta-Catenin/LEF1 activated enamelin expression in ameloblast-like cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2010,398(3):519-524.
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