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学科主题基础医学
beta-Catenin/LEF1 activated enamelin expression in ameloblast-like cells
Tian, Hua2,3; Lv, Ping2,3; Ma, Kangtao1; Zhou, Chunyan1; Gao, Xuejun2,3
关键词Enamelin Wnt/beta-catenin Lef1 Amelogenesis Imperfecta Tooth Development
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2010-07-30
DOI10.1016/j.bbrc.2010.06.111
398期:3页:519-524
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]DOMINANT AMELOGENESIS IMPERFECTA ; TISSUE-SPECIFIC EXPRESSION ; TOOTH MORPHOGENESIS ; MOUSE ; CATENIN ; MICE ; ESTABLISHMENT ; TRANSCRIPTION ; ELEMENTS ; LEF1
英文摘要

Enamelin is an ameloblast-specific matrix protein believed to play essential roles in enamel formation. However, mechanisms of enamelin transcription regulation are not clear. beta-Catenin/LEF1 is a key transcriptional complex involved in tooth development. In this study, the role of beta-catenin/LEF1 in enamelin expression was investigated. The 5′-flanking region of the mouse enamelin gene was analyzed and cloned. Co-transfection analysis and mutation assays revealed that two conserved LEF1 responsive elements located at -1002 and -597 bp upstream of the enamelin translation initiation site could augment transcriptional activity of the enamelin. The interaction between the enamelin elements and beta-catenin/LEF1 was further confirmed by electrophoresis mobility shift assays and chromatin immunoprecipitation assays. In addition, LiCl treatment induced nuclear translocation of beta-catenin and elevated endogenous enamelin expression in mouse ameloblast-like cells. The results suggested that Wnt/beta-catenin signaling could function in enamelin gene expression by direct interaction through two conserved LEF1 responsive elements on the enamelin gene in ameloblast-like cells. (C) 2010 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000280867400032
项目编号NSFC 30572033
资助机构Natural Science Foundation of China
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53500
专题北京大学基础医学院
北京大学第一临床医学院_人事处
北京大学口腔医学院_牙体牙髓科
作者单位1.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
2.Peking Univ, Sch Stomatol, Dept Cariol & Endodontol, Beijing 100191, Peoples R China
3.Peking Univ, Hosp Stomatol, Dept Cariol & Endodontol, Beijing 100191, Peoples R China
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GB/T 7714
Tian, Hua,Lv, Ping,Ma, Kangtao,et al. beta-Catenin/LEF1 activated enamelin expression in ameloblast-like cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2010,398(3):519-524.
APA Tian, Hua,Lv, Ping,Ma, Kangtao,Zhou, Chunyan,&Gao, Xuejun.(2010).beta-Catenin/LEF1 activated enamelin expression in ameloblast-like cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,398(3),519-524.
MLA Tian, Hua,et al."beta-Catenin/LEF1 activated enamelin expression in ameloblast-like cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 398.3(2010):519-524.
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