IR@PKUHSC  > 北京大学第一临床医学院  > 临床药理研究所
学科主题临床医学
A vancomycin-dependent VanA-type Enterococcus faecalis strain isolated in Japan from chicken imported from China
Tanimoto, K; Nomura, T; Hamatani, H; Xiao, YH; Ike, Y
关键词Chicken Meat D-ala : D-ala Ligase Vana-type Vancomycin-dependent Vancomycin-resistant Enterococci
刊名LETTERS IN APPLIED MICROBIOLOGY
2005
DOI10.1111/j.1472-765X.2005.01722.x
41期:2页:157-162
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology ; Microbiology
关键词[WOS]STREPTOCOCCUS-FAECALIS ; RESISTANT ENTEROCOCCI ; FAECIUM ; LIGASE ; PAD1 ; GENE
英文摘要

Aims: The characterization of KC122.1, which is a vancomycin-dependent VRE (Vancomycin-resistant enterococci) (Enterococcus faecalis) and the first case in Japan of a VRE isolate obtained from chicken meat imported from China.

Methods and Results: PCR amplification of vanA, vanS and ddl gene and direct sequencing of the PCR products were performed. KC122.1 was a VanA-type VRE showing high-level vancomycin resistance and low-level teicoplanin resistance, and its vanS gene had three point mutations. The ddl gene of KC122.1 was sequenced and two changes were found at the ninth codon (GCC-GAC) and the stop codon (TAA-CAA). The latter change was also found in the laboratory strain E. faecalis FA2-2.

Conclusions: Three point mutations in vanS resulted in high-level vancomycin resistance and low-level teicoplanin resistance. The change at the ninth codon resulted in the inactivation of the ddl gene and vancomycin-dependent growth. An eight amino acid extension at the C-terminal did not impair the function of the D-Ala : D-Ala ligase.

Significance and Impact of the Study: This is the first example of the isolation of VRE from chicken meat imported from China and the first vancomycin-dependent VRE from a nonhuman source.

语种英语
WOS记录号WOS:000230574100011
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53506
Collection北京大学第一临床医学院_临床药理研究所
作者单位1.Gunma Univ, Grad Sch Med, Lab Bacterial Drug Resistance, Gunma 3718511, Japan
2.Gunma Univ, Grad Sch Med, Dept Bacteriol & Bacterial Infect Control, Gunma 3718511, Japan
3.Peking Univ, Inst Clin Pharmacol, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Tanimoto, K,Nomura, T,Hamatani, H,et al. A vancomycin-dependent VanA-type Enterococcus faecalis strain isolated in Japan from chicken imported from China[J]. LETTERS IN APPLIED MICROBIOLOGY,2005,41(2):157-162.
APA Tanimoto, K,Nomura, T,Hamatani, H,Xiao, YH,&Ike, Y.(2005).A vancomycin-dependent VanA-type Enterococcus faecalis strain isolated in Japan from chicken imported from China.LETTERS IN APPLIED MICROBIOLOGY,41(2),157-162.
MLA Tanimoto, K,et al."A vancomycin-dependent VanA-type Enterococcus faecalis strain isolated in Japan from chicken imported from China".LETTERS IN APPLIED MICROBIOLOGY 41.2(2005):157-162.
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