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A vancomycin-dependent VanA-type Enterococcus faecalis strain isolated in Japan from chicken imported from China
作者: Tanimoto, K; Nomura, T; Hamatani, H; Xiao, YH; Ike, Y
关键词: chicken meat ; D-Ala : D-Ala ligase ; VanA-type ; vancomycin-dependent ; Vancomycin-resistant enterococci
刊名: LETTERS IN APPLIED MICROBIOLOGY
发表日期: 2005
DOI: 10.1111/j.1472-765X.2005.01722.x
卷: 41, 期:2, 页:157-162
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology ; Microbiology
关键词[WOS]: STREPTOCOCCUS-FAECALIS ; RESISTANT ENTEROCOCCI ; FAECIUM ; LIGASE ; PAD1 ; GENE
英文摘要:

Aims: The characterization of KC122.1, which is a vancomycin-dependent VRE (Vancomycin-resistant enterococci) (Enterococcus faecalis) and the first case in Japan of a VRE isolate obtained from chicken meat imported from China.

Methods and Results: PCR amplification of vanA, vanS and ddl gene and direct sequencing of the PCR products were performed. KC122.1 was a VanA-type VRE showing high-level vancomycin resistance and low-level teicoplanin resistance, and its vanS gene had three point mutations. The ddl gene of KC122.1 was sequenced and two changes were found at the ninth codon (GCC-GAC) and the stop codon (TAA-CAA). The latter change was also found in the laboratory strain E. faecalis FA2-2.

Conclusions: Three point mutations in vanS resulted in high-level vancomycin resistance and low-level teicoplanin resistance. The change at the ninth codon resulted in the inactivation of the ddl gene and vancomycin-dependent growth. An eight amino acid extension at the C-terminal did not impair the function of the D-Ala : D-Ala ligase.

Significance and Impact of the Study: This is the first example of the isolation of VRE from chicken meat imported from China and the first vancomycin-dependent VRE from a nonhuman source.

语种: 英语
WOS记录号: WOS:000230574100011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53506
Appears in Collections:北京大学第一临床医学院_临床药理研究所_期刊论文

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作者单位: 1.Gunma Univ, Grad Sch Med, Lab Bacterial Drug Resistance, Gunma 3718511, Japan
2.Gunma Univ, Grad Sch Med, Dept Bacteriol & Bacterial Infect Control, Gunma 3718511, Japan
3.Peking Univ, Inst Clin Pharmacol, Beijing 100871, Peoples R China

Recommended Citation:
Tanimoto, K,Nomura, T,Hamatani, H,et al. A vancomycin-dependent VanA-type Enterococcus faecalis strain isolated in Japan from chicken imported from China[J]. LETTERS IN APPLIED MICROBIOLOGY,2005,41(2):157-162.
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