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IR@PKUHSC  > 北京大学第一临床医学院  > 泌尿外科  > 期刊论文
学科主题: 临床医学
题名:
Expression and significance of histone H3K27 demethylases in renal cell carcinoma
作者: Shen, Yongqing1,5; Guo, Xiaoqiang1,2; Wang, Yuejia1; Qiu, Wei4; Chang, Yanzhong1; Zhang, Aili3; Duan, Xianglin1
关键词: Renal cell carcinoma ; Histone H3K27 demethylase ; UTX ; JMJD3 ; Epigenetics
刊名: BMC CANCER
发表日期: 2012-10-12
DOI: 10.1186/1471-2407-12-470
卷: 12
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: INDUCED SENESCENCE ; JMJD3 CONTRIBUTES ; INK4A-ARF LOCUS ; CANCER ; UTX ; SUPPRESSOR ; GENE ; INK4A/ARF ; PROTEINS
英文摘要:

Background: The histone H3K27 demethylases UTX and JMJD3 are important regulatory factors that modulate gene expression by altering the physical state of chromatin. Previous studies have indicated an abnormal H3K27 methylation status in carcinogenesis. We therefore investigated the expression patterns of UTX and JMJD3 in renal cell carcinoma (RCC) and their roles in cancer development.

Methods: The mRNA expression levels of the UTX and JMJD3 genes were determined in cancer tissues and adjacent normal tissues in 36 patients with primary RCC, using quantitative real-time-polymerase chain reaction. The UTX and JMJD3 protein contents were measured by western blotting and immunohistochemical analysis.

Results: UTX and JMJD3 transcripts were significantly increased in cancer tissues compared to normal tissues (P<0.05). mRNA levels of the inhibitor of cyclin-dependent kinases 4 and 6 p16INK4a were also increased in cancer tissues (P<0.001). Western blotting indicated that levels of both demethylases were increased in cancer tissues. The level of tri-methylated H3K27 (H3K27me3) was lower in cancer tissues compared to normal tissues, but expression of the H3K27 methyltransferase EZH2 was increased (P<0.05). These results suggest that the two H3K27 demethylases may play critical roles in the regulation of H3K27 methylation status in RCC. Immunohistochemical analysis confirmed that UTX and JMJD3 expression were upregulated in cancer tissues compared to adjacent tissues.

Conclusions: This study demonstrated that UTX and JMJD3 were upregulated in cancer tissues, suggesting that they may be involved in the development of primary RCC. The potential roles of H3K27 demethylases as biomarkers in the early diagnosis of RCC need to be further explored.

语种: 英语
所属项目编号: 30870265 ; C2010000410
项目资助者: National Natural Science Foundation of China ; Natural Science Foundation of Hebei Province
WOS记录号: WOS:000312308600002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53514
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Bethune Mil Med Coll, Dept Biochem, Shijiazhuang 050081, Peoples R China
2.Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China
3.Hebei Normal Univ, Coll Life Sci, Lab Iron Metab & Mol Biol, Shijiazhuang 050016, Peoples R China
4.Hebei Med Univ, Hosp 4, Dept Urol, Shijiazhuang 050011, Peoples R China
5.Hebei Med Univ, Xishan Coll, Shijiazhuang 050011, Peoples R China

Recommended Citation:
Shen, Yongqing,Guo, Xiaoqiang,Wang, Yuejia,et al. Expression and significance of histone H3K27 demethylases in renal cell carcinoma[J]. BMC CANCER,2012,12.
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