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学科主题: 基础医学
题名:
Insulin regulated the expression and activity of neuronal nitric oxide synthase in PC12 cells
作者: Yuan, ZR; Lu, DD; Dong, XM; Guli, N; Cheng, S; Lu, JF
关键词: insulin ; neuronal nitric oxide synthase ; flow cytometry ; in situ hybridization ; ESR ; PC12 cells
刊名: PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
发表日期: 2003-12-01
卷: 30, 期:6, 页:960-964
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: GENE-EXPRESSION ; IN-VIVO
英文摘要:

To research the effect of insulin on the expression and activity of the neuronal nitric oxide synthase in neural cells, the effects of insulin on the expression and activity of neuronal nitric oxide synthase were investigated in PC 12 cells, by using flow cytometry, in situ hybridization and electron spin resonance (ESR) techniques. After incubation with insulin for 9 h, the expression of nNOS protein was significantly increased by insulin in a concentration-dependent manner, with a maximal increase of about 55% of the control values (P < 0.01, n = 3, t-test). The transcription of nNOS was also significantly increased by insulin (16 mU/L, 6 h), reaching (182 &PLUSMN; 13)% of the control values (P <0.01, n = 3, t-test). In addition, significant increase of the activity of nNOS in PC12 cells was also detected after incubation with insulin (16 mU/L) for 9 h, with an increase of 67% of the control values (P < 0.01, n = 4, t-test). It can be concluded that insulin can upregulate the expression and activity of neuronal nitric oxide synthase in PC12 cells.

语种: 中文
WOS记录号: WOS:000224108900026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53533
Appears in Collections:基础医学院_细胞生物学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
2.Shandong Univ, Coll Basic Med Sci, Dept Pathophysiol, Jinan 250012, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Cell Biol, Beijing 100083, Peoples R China

Recommended Citation:
Yuan, ZR,Lu, DD,Dong, XM,et al. Insulin regulated the expression and activity of neuronal nitric oxide synthase in PC12 cells[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2003,30(6):960-964.
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