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学科主题基础医学
Insulin regulated the expression and activity of neuronal nitric oxide synthase in PC12 cells
Yuan, ZR; Lu, DD; Dong, XM; Guli, N; Cheng, S; Lu, JF
关键词Insulin Neuronal Nitric Oxide Synthase Flow Cytometry In Situ Hybridization Esr Pc12 Cells
刊名PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
2003-12-01
30期:6页:960-964
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]GENE-EXPRESSION ; IN-VIVO
英文摘要

To research the effect of insulin on the expression and activity of the neuronal nitric oxide synthase in neural cells, the effects of insulin on the expression and activity of neuronal nitric oxide synthase were investigated in PC 12 cells, by using flow cytometry, in situ hybridization and electron spin resonance (ESR) techniques. After incubation with insulin for 9 h, the expression of nNOS protein was significantly increased by insulin in a concentration-dependent manner, with a maximal increase of about 55% of the control values (P < 0.01, n = 3, t-test). The transcription of nNOS was also significantly increased by insulin (16 mU/L, 6 h), reaching (182 &PLUSMN; 13)% of the control values (P <0.01, n = 3, t-test). In addition, significant increase of the activity of nNOS in PC12 cells was also detected after incubation with insulin (16 mU/L) for 9 h, with an increase of 67% of the control values (P < 0.01, n = 4, t-test). It can be concluded that insulin can upregulate the expression and activity of neuronal nitric oxide synthase in PC12 cells.

语种中文
WOS记录号WOS:000224108900026
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53533
专题北京大学基础医学院_细胞生物学系
作者单位1.Peking Univ, Hlth Sci Ctr, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
2.Shandong Univ, Coll Basic Med Sci, Dept Pathophysiol, Jinan 250012, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Cell Biol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Yuan, ZR,Lu, DD,Dong, XM,et al. Insulin regulated the expression and activity of neuronal nitric oxide synthase in PC12 cells[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2003,30(6):960-964.
APA Yuan, ZR,Lu, DD,Dong, XM,Guli, N,Cheng, S,&Lu, JF.(2003).Insulin regulated the expression and activity of neuronal nitric oxide synthase in PC12 cells.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,30(6),960-964.
MLA Yuan, ZR,et al."Insulin regulated the expression and activity of neuronal nitric oxide synthase in PC12 cells".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 30.6(2003):960-964.
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