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The Staphylococcus aureus GGDEF Domain-Containing Protein, GdpS, Influences Protein A Gene Expression in a Cyclic Diguanylic Acid-Independent Manner
Shang, Fei1,2; Xue, Ting1,2; Sun, Haipeng1,2; Xing, Lei3; Zhang, Shuo3; Yang, Zhenjun3; Zhang, Lihe3; Sun, Baolin1,2
刊名INFECTION AND IMMUNITY
2009-07-01
DOI10.1128/IAI.01405-08
77期:7页:2849-2856
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology ; Infectious Diseases
研究领域[WOS]Immunology ; Infectious Diseases
关键词[WOS]C-DI-GMP ; BIOFILM FORMATION ; VIRULENCE ; AGR ; IDENTIFICATION ; AUTOLYSIS ; BACTERIA ; LOCUS ; REGULATOR ; SYSTEM
英文摘要

Staphylococcus aureus is an important human pathogen that is the principal cause of a variety of diseases, ranging from localized skin infections to life-threatening systemic infections. The success of the organism as a pathogen and its ability to cause such a wide range of infections are due to its extensive virulence factors. In this study, we identified the role of the only GGDEF domain protein (GdpS [GGDEF domain protein from Staphylococcus]) in the virulence of S. aureus NCTC8325. Inactivation of gdpS results in an alteration in the production of a range of virulence factors, such as serine and cysteine proteases, fibrinogen-binding proteins, and, specifically, protein A (Spa), a major surface protein of S. aureus. The transcript level of spa decreases eightfold in the gdpS mutant compared with the parental NCTC8325 strain. Furthermore, the transcript level of sarS, which encodes a direct positive regulator of spa, also decreases in the gdpS mutant compared with the wild type, while the transcript levels of agr, sarA, sarT, and rot display no apparent changes in the gdpS mutant, suggesting that GdpS affects the expression of spa through interaction with SarS by unknown mechanisms. Furthermore, the complementation assays show that the influences of GdpS on spa and sarS depend on its N-terminal domain, which is predicted to be the sensor of a two-component system, rather than its C-terminal GGDEF domain with conserved GGDEF, suggesting that GdpS functions in S. aureus by an unknown mechanism independent of 3′,5′-cyclic diguanylic acid signaling.

语种英语
WOS记录号WOS:000267227000027
项目编号2004CB518904 ; 30721002
资助机构One Hundred Talent Project of the Chinese Academy of Sciences ; Ministry of Science and Technology of China ; National Natural Science Foundation of China
引用统计
被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53536
专题北京大学药学院
北京大学药学院_药物化学系
北京大学口腔医学院_第三门诊部
作者单位1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China
2.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China
3.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Shang, Fei,Xue, Ting,Sun, Haipeng,et al. The Staphylococcus aureus GGDEF Domain-Containing Protein, GdpS, Influences Protein A Gene Expression in a Cyclic Diguanylic Acid-Independent Manner[J]. INFECTION AND IMMUNITY,2009,77(7):2849-2856.
APA Shang, Fei.,Xue, Ting.,Sun, Haipeng.,Xing, Lei.,Zhang, Shuo.,...&Sun, Baolin.(2009).The Staphylococcus aureus GGDEF Domain-Containing Protein, GdpS, Influences Protein A Gene Expression in a Cyclic Diguanylic Acid-Independent Manner.INFECTION AND IMMUNITY,77(7),2849-2856.
MLA Shang, Fei,et al."The Staphylococcus aureus GGDEF Domain-Containing Protein, GdpS, Influences Protein A Gene Expression in a Cyclic Diguanylic Acid-Independent Manner".INFECTION AND IMMUNITY 77.7(2009):2849-2856.
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