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学科主题: 药学
题名:
The Staphylococcus aureus GGDEF Domain-Containing Protein, GdpS, Influences Protein A Gene Expression in a Cyclic Diguanylic Acid-Independent Manner
作者: Shang, Fei1,2; Xue, Ting1,2; Sun, Haipeng1,2; Xing, Lei3; Zhang, Shuo3; Yang, Zhenjun3; Zhang, Lihe3; Sun, Baolin1,2
刊名: INFECTION AND IMMUNITY
发表日期: 2009-07-01
DOI: 10.1128/IAI.01405-08
卷: 77, 期:7, 页:2849-2856
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology ; Infectious Diseases
研究领域[WOS]: Immunology ; Infectious Diseases
关键词[WOS]: C-DI-GMP ; BIOFILM FORMATION ; VIRULENCE ; AGR ; IDENTIFICATION ; AUTOLYSIS ; BACTERIA ; LOCUS ; REGULATOR ; SYSTEM
英文摘要:

Staphylococcus aureus is an important human pathogen that is the principal cause of a variety of diseases, ranging from localized skin infections to life-threatening systemic infections. The success of the organism as a pathogen and its ability to cause such a wide range of infections are due to its extensive virulence factors. In this study, we identified the role of the only GGDEF domain protein (GdpS [GGDEF domain protein from Staphylococcus]) in the virulence of S. aureus NCTC8325. Inactivation of gdpS results in an alteration in the production of a range of virulence factors, such as serine and cysteine proteases, fibrinogen-binding proteins, and, specifically, protein A (Spa), a major surface protein of S. aureus. The transcript level of spa decreases eightfold in the gdpS mutant compared with the parental NCTC8325 strain. Furthermore, the transcript level of sarS, which encodes a direct positive regulator of spa, also decreases in the gdpS mutant compared with the wild type, while the transcript levels of agr, sarA, sarT, and rot display no apparent changes in the gdpS mutant, suggesting that GdpS affects the expression of spa through interaction with SarS by unknown mechanisms. Furthermore, the complementation assays show that the influences of GdpS on spa and sarS depend on its N-terminal domain, which is predicted to be the sensor of a two-component system, rather than its C-terminal GGDEF domain with conserved GGDEF, suggesting that GdpS functions in S. aureus by an unknown mechanism independent of 3′,5′-cyclic diguanylic acid signaling.

语种: 英语
所属项目编号: 2004CB518904 ; 30721002
项目资助者: One Hundred Talent Project of the Chinese Academy of Sciences ; Ministry of Science and Technology of China ; National Natural Science Foundation of China
WOS记录号: WOS:000267227000027
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53536
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China
2.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China
3.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100083, Peoples R China

Recommended Citation:
Shang, Fei,Xue, Ting,Sun, Haipeng,et al. The Staphylococcus aureus GGDEF Domain-Containing Protein, GdpS, Influences Protein A Gene Expression in a Cyclic Diguanylic Acid-Independent Manner[J]. INFECTION AND IMMUNITY,2009,77(7):2849-2856.
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