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学科主题: 药学
题名:
Mastoparan/Mastoparan X altered binding behavior of La3+ to calmodulin in ternary complexes
作者: Yang, Qin; Hu, Jian; Yang, Xiaoda1; Wang, Kui
关键词: calmodulin ; lanthanum ; CaM binding peptide ; Mastoparan
刊名: JOURNAL OF INORGANIC BIOCHEMISTRY
发表日期: 2008-02-01
DOI: 10.1016/j.jinorgbio.2007.08.011
卷: 102, 期:2, 页:278-284
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Chemistry, Inorganic & Nuclear
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: LANTHANIDE AFFINITY ; CA2+ BINDING ; CALCIUM ; MASTOPARAN ; PEPTIDES ; ALUMINUM ; ACTIVATION ; FRAGMENT ; DIALYSIS ; DOMAINS
英文摘要:

Ca2+ binds to calmodulin (CaM) and triggers the interaction of CaM with its target proteins; CaM binding proteins (CaMBPs) can also regulate the metal binding to CaM. In the present paper, La3+ binding to CaM was studied in the presence of the CaM binding peptides, Mastoparan (Mas) and Mas X, using ultrafiltration and titration of fluorescence. Ca2+ binding was used as an analog to understand La3+ binding in intact CaM and isolated N/C-terminal CaM domain of metal-CaM binary system and metal-CaM-CaMBPs ternary system. Mas/Mas X increased binding affinity of La3+ to CaM by 0.5 similar to 3 orders magnitude. The metal ions binding affinity to the C-terminal or the N-terminal CaM domain suggested that in the first phase of binding process both Ca2+ and La3+ bind to C-terminal of CaM in the presence of Mas/Mas X. In the presence of CaM binding peptides, La3+ binding preference was substantially altered from the metal-CaM binary system where La3+ slightly preferred binding to the N-terminal sites of CaM. Our results will be helpful in understanding La3+ interactions with CaM in the biological systems. (c) 2007 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000253553000014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53537
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Labs Nat & Biomimet Drugs, Beijing 100083, Peoples R China

Recommended Citation:
Yang, Qin,Hu, Jian,Yang, Xiaoda,et al. Mastoparan/Mastoparan X altered binding behavior of La3+ to calmodulin in ternary complexes[J]. JOURNAL OF INORGANIC BIOCHEMISTRY,2008,102(2):278-284.
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