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学科主题: 药学
题名:
Determination of paeoniflorin in rat plasma by a liquid chromatography-tandem mass spectrometry method coupled with solid-phase extraction
作者: Wang, Q; Yang, HY; Liu, WN; Feng, XL; Zhang, LT; Li, YL; Bi, KS; Guo, D
关键词: solid-phase extraction ; paeoniflorin ; HPLC-MS-MS
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 2006-02-01
DOI: 10.1002/bmc.548
卷: 20, 期:2, 页:173-179
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: PAEONIAE RADIX ; GLYCYRRHIZIN ; MICE ; PHARMACOKINETICS ; DECOCTION ; MUSCLE ; SERUM ; ROOT
英文摘要:

A rapid, sensitive and selective liquid chromatography-tandem spectrometry method was developed and validated for determination of paeoniflorin in rat plasma using geniposide as the internal standard. The samples were pretreated with solid-phase extraction using Extract-Clean (TM) cartridges. Separation of paeoniflorin and IS was achieved on a reversed-phase C-18 column (50 x 4.6 mm i.d.) with a mobile phase made up of acetonitrile and 0.05% formic acid (25:75, v/v) at a flow rate of 0.5 mL/min. Detection was carried out on a triple quadrupole tandem mass spectrometer by multiple-reaction monitoring and an electrospray ionization source was employed as the ionization source. The lower limit of quantification obtained was 4 ng/mL (n = 6) using 200 mu L plasma with an accuracy of -3.67% (relative error) and a precision of 4.13% (relative standard deviation). A good linearity was found in the range of 4-1000 ng/mL. The intra- and inter-day relative standard deviations in the measurement of quality control samples 10, 150 and 800 ng/mL ranged from 3.73 to 4.94% and from 4.31 to 6.56%, respectively. The accuracy was from -3.93 to -1.11% in terms of relative error. The analyte and IS were stable in the battery of stability studies. This method was successfully applied to a pharmacokinetic study of paeoniflorin after a single oral administration of 53.36 mg/kg paeoniflorin to rats. Copyright (c) 2005 John Wiley & Sons, Ltd.

语种: 英语
WOS记录号: WOS:000235405300005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53569
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
2.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China
3.Hebei Med Univ, Sch Pharm, Shijiazhuang 050017, Peoples R China
4.Zhongqi Pharmaceut Technol Shijiazhuang Co Ltd, Shijiazhuang 050051, Peoples R China

Recommended Citation:
Wang, Q,Yang, HY,Liu, WN,et al. Determination of paeoniflorin in rat plasma by a liquid chromatography-tandem mass spectrometry method coupled with solid-phase extraction[J]. BIOMEDICAL CHROMATOGRAPHY,2006,20(2):173-179.
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