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Determination of paeoniflorin in rat plasma by a liquid chromatography-tandem mass spectrometry method coupled with solid-phase extraction
Wang, Q; Yang, HY; Liu, WN; Feng, XL; Zhang, LT; Li, YL; Bi, KS; Guo, D
关键词Solid-phase Extraction Paeoniflorin Hplc-ms-ms
刊名BIOMEDICAL CHROMATOGRAPHY
2006-02-01
DOI10.1002/bmc.548
20期:2页:173-179
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]PAEONIAE RADIX ; GLYCYRRHIZIN ; MICE ; PHARMACOKINETICS ; DECOCTION ; MUSCLE ; SERUM ; ROOT
英文摘要

A rapid, sensitive and selective liquid chromatography-tandem spectrometry method was developed and validated for determination of paeoniflorin in rat plasma using geniposide as the internal standard. The samples were pretreated with solid-phase extraction using Extract-Clean (TM) cartridges. Separation of paeoniflorin and IS was achieved on a reversed-phase C-18 column (50 x 4.6 mm i.d.) with a mobile phase made up of acetonitrile and 0.05% formic acid (25:75, v/v) at a flow rate of 0.5 mL/min. Detection was carried out on a triple quadrupole tandem mass spectrometer by multiple-reaction monitoring and an electrospray ionization source was employed as the ionization source. The lower limit of quantification obtained was 4 ng/mL (n = 6) using 200 mu L plasma with an accuracy of -3.67% (relative error) and a precision of 4.13% (relative standard deviation). A good linearity was found in the range of 4-1000 ng/mL. The intra- and inter-day relative standard deviations in the measurement of quality control samples 10, 150 and 800 ng/mL ranged from 3.73 to 4.94% and from 4.31 to 6.56%, respectively. The accuracy was from -3.93 to -1.11% in terms of relative error. The analyte and IS were stable in the battery of stability studies. This method was successfully applied to a pharmacokinetic study of paeoniflorin after a single oral administration of 53.36 mg/kg paeoniflorin to rats. Copyright (c) 2005 John Wiley & Sons, Ltd.

语种英语
WOS记录号WOS:000235405300005
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53569
专题北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
2.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China
3.Hebei Med Univ, Sch Pharm, Shijiazhuang 050017, Peoples R China
4.Zhongqi Pharmaceut Technol Shijiazhuang Co Ltd, Shijiazhuang 050051, Peoples R China
推荐引用方式
GB/T 7714
Wang, Q,Yang, HY,Liu, WN,et al. Determination of paeoniflorin in rat plasma by a liquid chromatography-tandem mass spectrometry method coupled with solid-phase extraction[J]. BIOMEDICAL CHROMATOGRAPHY,2006,20(2):173-179.
APA Wang, Q.,Yang, HY.,Liu, WN.,Feng, XL.,Zhang, LT.,...&Guo, D.(2006).Determination of paeoniflorin in rat plasma by a liquid chromatography-tandem mass spectrometry method coupled with solid-phase extraction.BIOMEDICAL CHROMATOGRAPHY,20(2),173-179.
MLA Wang, Q,et al."Determination of paeoniflorin in rat plasma by a liquid chromatography-tandem mass spectrometry method coupled with solid-phase extraction".BIOMEDICAL CHROMATOGRAPHY 20.2(2006):173-179.
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