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学科主题: 药学
题名:
Pressurized liquid extraction followed by high-performance liquid chromatography for determination of seven active compounds in Cortex Dictamni
作者: Jiang, Y; Li, SP; Chang, HT; Wang, YT; Tu, PF
关键词: Cortex Dictamni ; PLE ; HPLC ; content determination ; quality control
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2006-03-10
DOI: 10.1016/j.chroma.2006.01.077
卷: 1108, 期:2, 页:268-272
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: MEDICINAL-PLANTS ; SOLVENT-EXTRACTION ; DASYCARPUS
英文摘要:

A new method based on pressurized liquid extraction (PLE) followed by a sensitive and specific HPLC-DAD analysis is developed for determination of seven compounds in Cortex Dictamni. The operational parameters of PLE, such as extraction solvent, extraction temperature, extraction pressure, static extraction time, flush volume and extraction cycles were optimized, using the extraction efficiencies of dictamnine, obacunone and fraxinellone as targets. The optimized procedure employed MeOH as extraction solvent, 150 degrees C of extraction temperature, 1500psi extraction pressure, 5 min of static extraction time, 60% flush volume and the extraction recoveries of the three compounds were nearly to 100% for only one cycle. The following HPLC analysis was performed on a reversed-phase C-18 column with methanol-water as mobile phase in gradient manner, detected at 236 and 218 nm. The limits of detection (LOD) and limits of quantification (LOQ) of the seven compounds were in the range of 0.4-15.6 ng and 1.2-38.8 ng. This assay can be readily utilized as a quality control method for Cortex Dictamni and other related medicinal plants. (c) 2006 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000235854900018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53570
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Beijing 100871, Peoples R China
2.Univ Macau, Inst Chinese Med Sci, Taipa, Macau, Peoples R China

Recommended Citation:
Jiang, Y,Li, SP,Chang, HT,et al. Pressurized liquid extraction followed by high-performance liquid chromatography for determination of seven active compounds in Cortex Dictamni[J]. JOURNAL OF CHROMATOGRAPHY A,2006,1108(2):268-272.
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