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学科主题: 临床医学
题名:
An oncolytic adenovirus expressing herpes simplex virus-thymidine kinase for targeting cancer therapy: An in vitro evaluation
作者: Zheng, Fei-qun1; Xu, Yin2; Qin, Yi-de2; Yang, Ren-jie1; Han, Jun3
关键词: Conditionally replicative adenovirus ; Cancer gene therapy ; Herpex simplex virus-thymidine kinase
刊名: CHINESE JOURNAL OF CANCER RESEARCH
发表日期: 2009-06-01
DOI: 10.1007/s11670-009-0090-z
卷: 21, 期:2, 页:90-96
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: SUICIDE GENE-THERAPY ; ANTITUMOR EFFICACY ; COLON-CANCER ; PROMOTER ; VECTORS ; CYTOTOXICITY ; CELLS
英文摘要:

Oncolytic adenovirus, also called conditionally replicating adenovirus (CRAD), has been developed for the treatment of cancer. However, there is a tremendous need to enhance their antitumor efficacy. Here we wish to evaluate whether a strategy that combines the herpes simplex virus-thymidine kinase with oncolytic effects offers a therapeutic advantage.

A novel adenovirus Ad-ETK containing a sequentially positioned promoter of human telomerase reverse transcriptase (hTERT), the coding sequence of E1A gene, an internal ribosome entry site sequence (IRES) and the coding sequence of herpes simplex virus-thymidine kinase (HSV-TK) was constructed. Infection of various cells with Ad-ETK followed by RT-PCR confirmed the expression of E1A and HSV-TK. The oncolytic ability and synergism between oncolytic effects and HSV-TK system was measured. The infection efficiency was determined by flow cytometry.

Ad-ETK deliverys E1A and HSV-TK gene, which selectively replicates in hTERT-positive tumor cells, and the progeny virus can reach up to 150 IU/cell. Our in vitro study showed that Ad-ETK plus ganciclovir (GCV) induced an obvious cell death.

An oncolytic adenovirus plus the HSV-TK/GCV suicide gene system resulted in a significant improvement in treatment efficacy and it may offer important considerations in the development and preclinical assessments of oncolytic virotherapy.

语种: 英语
WOS记录号: WOS:000266946900002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53581
Appears in Collections:北京大学临床肿瘤学院_介入治疗科_期刊论文

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作者单位: 1.Peking Univ, Key Lab Carcinogenesis & Translat Res, Dept Intervent Therapy, Minist Educ,Sch Oncol,Beijing Canc Hosp & Inst, Beijing 100142, Peoples R China
2.Anhui Med Univ, Dept Biochem & Mol Biol, Hefei 230032, Anhui, Peoples R China
3.Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 100052, Peoples R China

Recommended Citation:
Zheng, Fei-qun,Xu, Yin,Qin, Yi-de,et al. An oncolytic adenovirus expressing herpes simplex virus-thymidine kinase for targeting cancer therapy: An in vitro evaluation[J]. CHINESE JOURNAL OF CANCER RESEARCH,2009,21(2):90-96.
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