IR@PKUHSC  > 北京大学第三临床医学院  > 生殖医学中心
学科主题临床医学
Improved Efficiency of Microsurgical Enucleated Tripronuclear Zygotes Development and Embryonic Stem Cell Derivation by Supplementing Epidermal Growth Factor, Brain-Derived Neurotrophic Factor, and Insulin-Like Growth Factor-1
Fan, Yong1; Li, Rong2,3; Huang, Jin2,4; Zhao, Hong-Cui2,3; Ding, Ting2,3; Sun, Xiaofang1; Yu, Yang2,3; Qiao, Jie2,3,4
刊名STEM CELLS AND DEVELOPMENT
2014-03-15
DOI10.1089/scd.2013.0420
23期:6页:563-575
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell & Tissue Engineering ; Hematology ; Medicine, Research & Experimental ; Transplantation
研究领域[WOS]Cell Biology ; Hematology ; Research & Experimental Medicine ; Transplantation
关键词[WOS]COMPARATIVE GENOMIC HYBRIDIZATION ; HUMAN OOCYTE MATURATION ; POOR-QUALITY EMBRYOS ; 4-CELL STAGE EMBRYOS ; VITRO MATURATION ; PREIMPLANTATION EMBRYO ; MOUSE OOCYTES ; LINES ; PROMOTES ; BLASTOCYSTS
英文摘要

Human embryonic stem cells (hESCs) hold great promise for future clinical cell therapies because of their unique potential to differentiate into all human cell types. However, the destruction of normal fertilized embryos and the derivation of hESCs for research has resulted in polarized ethical debates, with most of the controversy centered on embryo destruction. Therefore, due to less ethical controversy surrounding them, abnormal fertilized zygotes that are usually discarded are a potential feasible resource for the derivation of hESCs. Microsurgery on human polyspermic zygotes can contribute to the derivation of hESCs, but the efficiency is much lower. Here, we reported a culture system to enhance the developmental competence of such microsurgical human polyspermic zygotes by EGF-BDNF-IGF-1 combination, which eventually resulted in the increased derivation efficiency of hESCs from them. We found that the developmental efficiency of microsurgical enucleated tripronuclear (3PN) embryos cultured with the EGF-BDNF-IGF-1 combination was significantly increased compared with the control group. More importantly, when the microsurgical enucleated 3PN embryos were cultured in medium supplemented with EGF-BDNF-IGF-1, the frequency ratio of chromosome abnormality was reduced. Our present study will facilitate the development of hESC line derivation in subsequent studies and also provide an additional choice for infertile couples.

语种英语
WOS记录号WOS:000332095900001
项目编号2011CB944504 ; 2014CB943203 ; 20110001120008 ; 31371521 ; 81100473 ; 81370766 ; U1132005 ; S2013010014781 ; 2012J2200006
资助机构Ministry of Science and Technology of China (973 program) ; Specialized Research Fund for the Doctoral Program of Higher Education ; National Natural Science Foundation of China ; Guangdong Natural Science Funds ; Zhujiang Science and Technology Star Project of Guangzhou
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53595
专题北京大学第三临床医学院_生殖医学中心
北京大学第二临床医学院_麻醉科
北京大学第三临床医学院_妇产科
作者单位1.Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China
2.Guangzhou Med Univ, Affiliated Hosp 3, Key Lab Major Obstet Dis Guangdong Prov, Guangzhou, Guangdong, Peoples R China
3.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100191, Peoples R China
4.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Fan, Yong,Li, Rong,Huang, Jin,et al. Improved Efficiency of Microsurgical Enucleated Tripronuclear Zygotes Development and Embryonic Stem Cell Derivation by Supplementing Epidermal Growth Factor, Brain-Derived Neurotrophic Factor, and Insulin-Like Growth Factor-1[J]. STEM CELLS AND DEVELOPMENT,2014,23(6):563-575.
APA Fan, Yong.,Li, Rong.,Huang, Jin.,Zhao, Hong-Cui.,Ding, Ting.,...&Qiao, Jie.(2014).Improved Efficiency of Microsurgical Enucleated Tripronuclear Zygotes Development and Embryonic Stem Cell Derivation by Supplementing Epidermal Growth Factor, Brain-Derived Neurotrophic Factor, and Insulin-Like Growth Factor-1.STEM CELLS AND DEVELOPMENT,23(6),563-575.
MLA Fan, Yong,et al."Improved Efficiency of Microsurgical Enucleated Tripronuclear Zygotes Development and Embryonic Stem Cell Derivation by Supplementing Epidermal Growth Factor, Brain-Derived Neurotrophic Factor, and Insulin-Like Growth Factor-1".STEM CELLS AND DEVELOPMENT 23.6(2014):563-575.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Fan, Yong]的文章
[Li, Rong]的文章
[Huang, Jin]的文章
百度学术
百度学术中相似的文章
[Fan, Yong]的文章
[Li, Rong]的文章
[Huang, Jin]的文章
必应学术
必应学术中相似的文章
[Fan, Yong]的文章
[Li, Rong]的文章
[Huang, Jin]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。