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学科主题: 临床医学
题名:
Improved Efficiency of Microsurgical Enucleated Tripronuclear Zygotes Development and Embryonic Stem Cell Derivation by Supplementing Epidermal Growth Factor, Brain-Derived Neurotrophic Factor, and Insulin-Like Growth Factor-1
作者: Fan, Yong1; Li, Rong2,3; Huang, Jin2,4; Zhao, Hong-Cui2,3; Ding, Ting2,3; Sun, Xiaofang1; Yu, Yang2,3; Qiao, Jie2,3,4
刊名: STEM CELLS AND DEVELOPMENT
发表日期: 2014-03-15
DOI: 10.1089/scd.2013.0420
卷: 23, 期:6, 页:563-575
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering ; Hematology ; Medicine, Research & Experimental ; Transplantation
研究领域[WOS]: Cell Biology ; Hematology ; Research & Experimental Medicine ; Transplantation
关键词[WOS]: COMPARATIVE GENOMIC HYBRIDIZATION ; HUMAN OOCYTE MATURATION ; POOR-QUALITY EMBRYOS ; 4-CELL STAGE EMBRYOS ; VITRO MATURATION ; PREIMPLANTATION EMBRYO ; MOUSE OOCYTES ; LINES ; PROMOTES ; BLASTOCYSTS
英文摘要:

Human embryonic stem cells (hESCs) hold great promise for future clinical cell therapies because of their unique potential to differentiate into all human cell types. However, the destruction of normal fertilized embryos and the derivation of hESCs for research has resulted in polarized ethical debates, with most of the controversy centered on embryo destruction. Therefore, due to less ethical controversy surrounding them, abnormal fertilized zygotes that are usually discarded are a potential feasible resource for the derivation of hESCs. Microsurgery on human polyspermic zygotes can contribute to the derivation of hESCs, but the efficiency is much lower. Here, we reported a culture system to enhance the developmental competence of such microsurgical human polyspermic zygotes by EGF-BDNF-IGF-1 combination, which eventually resulted in the increased derivation efficiency of hESCs from them. We found that the developmental efficiency of microsurgical enucleated tripronuclear (3PN) embryos cultured with the EGF-BDNF-IGF-1 combination was significantly increased compared with the control group. More importantly, when the microsurgical enucleated 3PN embryos were cultured in medium supplemented with EGF-BDNF-IGF-1, the frequency ratio of chromosome abnormality was reduced. Our present study will facilitate the development of hESC line derivation in subsequent studies and also provide an additional choice for infertile couples.

语种: 英语
所属项目编号: 2011CB944504 ; 2014CB943203 ; 20110001120008 ; 31371521 ; 81100473 ; 81370766 ; U1132005 ; S2013010014781 ; 2012J2200006
项目资助者: Ministry of Science and Technology of China (973 program) ; Specialized Research Fund for the Doctoral Program of Higher Education ; National Natural Science Foundation of China ; Guangdong Natural Science Funds ; Zhujiang Science and Technology Star Project of Guangzhou
WOS记录号: WOS:000332095900001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53595
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China
2.Guangzhou Med Univ, Affiliated Hosp 3, Key Lab Major Obstet Dis Guangdong Prov, Guangzhou, Guangdong, Peoples R China
3.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100191, Peoples R China
4.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing, Peoples R China

Recommended Citation:
Fan, Yong,Li, Rong,Huang, Jin,et al. Improved Efficiency of Microsurgical Enucleated Tripronuclear Zygotes Development and Embryonic Stem Cell Derivation by Supplementing Epidermal Growth Factor, Brain-Derived Neurotrophic Factor, and Insulin-Like Growth Factor-1[J]. STEM CELLS AND DEVELOPMENT,2014,23(6):563-575.
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